α-chloro-2-fluorotoluene

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

From 12 NOV 2019 to 05 JUN 2020

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2020

Report date:

2020

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Version / remarks:

Adopted April 13, 2004

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Reference substance 001

Cas Number:

345-35-7

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

- Concentrations:
50, 20, 10, 5 and 1 mg test item/L and a control
corresponding to following time weighted average concentrations of the test item:
14, 4.96, 2.68, 1.19 and 0.292 mg test item/L, and a control.
- Sampling method: Duplicate samples from the freshly prepared test media of all test concentrations and the control were taken at the start of the test. All samples were diluted by a factor of 2 with acetonitrile.
- Sample storage conditions before analysis: All samples were stored in a freezer (≤ - 20 °C), protected from light, until analysis was performed.

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION:
- Method: A solution of the highest test item concentration of 50 mg test item/L was prepared by dissolving 47.4 mg test item into 948 mL test water by intense stirring for 4 hours and ultrasonic treatment for 10 minutes. Adequate volumes of this stock solution were diluted with test water to prepare the test media of the desired test concentrations.
- Controls: In the control, test water was used without addition of the test item.
- Evidence of undissolved material: No remarkable observations, clear test medium

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna
- Clone: clone 5
- Age at study initiation: 0.25 to 21.25 h old. Not first brood progeny.
- Method of breeding: The daphnids were bred in the laboratories of ibacon under similar temperature and light conditions as used in the test. The cultivation of the parental daphnids was performed in Elendt M4 medium. The daphnids in the stock culture were fed at least on all working days with green algae (Desmodesmus subspicatus) freshly grown in the laboratories of ibacon.
- Source: ibacon's in-house laboratory culture
- Feeding during test : None

ACCLIMATION
Not necessary, since the test was performed in the same medium as the culturing.

Study design

Test type:

static

Water media type:

freshwater

Remarks:

Reconstitued water (Elendt "M4")

Limit test:

no

Total exposure duration:

48 h

Test conditions

Hardness:

2.5 mmol/L (= 250 mg/L) as CaCO3

Test temperature:

Water Temperature:
19.2 to 20.0 °C at test start; 19.3 to 19.4 °C at test end

pH:

7.7 to 8.0 at test start; 7.8 to 7.9 at test end

Dissolved oxygen:

8.7 to 9.1 mg/L at test start; 8.9 to 9.0 mg/L at test end

Nominal and measured concentrations:

Nominal: 50, 20, 10, 5 and 1 mg test item/L
Time weighted average concentrations: 14, 4.96, 2.68, 1.19 and 0.292 mg test item/L

Details on test conditions:

TEST SYSTEM
- Test vessel: Beakers
- Material, size, headspace, fill volume: Glass beakers of 100 mL volume
- Volume of solution: approx. 60 mL test medium
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Reconstituted Water (Elendt "M4")

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 h light - 8 h dark
- Light intensity: 390 to 710 lux

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : The mobility of the daphnids was determined by visual observation after 24 and 48 hours. Those animals that are not able to swim within 15 seconds after gentle agitation of the test beaker were considered to be immobile (even if they could still move their antennae).

Reference substance (positive control):

yes

Remarks:

Potassium dichromate (tested at least twice a year)

Results and discussion

Effect concentrations

Effect concentrations 1

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

0.367 mg/L

Conc. based on:

act. ingr.

Basis for effect:

mobility

Effect concentrations 2

Key result

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

0.367 mg/L

Conc. based on:

act. ingr.

Basis for effect:

mobility

Details on results:

. - Immobilisation of control: 0%
- Other adverse effects control: no signs of disease or stress

Results with reference substance (positive control):

- Results with reference substance valid? Yes
- 24-h EC50 = 0.707 mg/L (95% CL: 0.608-0.822 mg/L)
- 48-h EC50 = 0.542 mg/L (95% CL: n.d.)

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Remarks:

Control Immobilisation Rate: 0 %,no daphnid showed signs of disease or stress; thus the validity criterion was met. Dissolved Oxygen Concentration: ≥ 8.9 mg O2/L in in all treatment groups at the end of the test; thus validity criterion was met.

Conclusions:

The toxic effect of the test item 2-Fluorobenzyl chloride to Daphnia magna was assessed in a static concentration-response test. The 48-hour NOEC was determined to be < 0.292 mg test item/L. The 48-hour LOEC was determined to be ≤ 0.292 mg test item/L and the 48-hour EC50 value was calculated to be 0.367 mg test item/L.

Executive summary:

The purpose of this study was to determine the influence of the test item 2-Fluorobenzyl chloride on the mobility of Daphnia magna. For this purpose, young daphnids (< 24 hours old) were exposed in a static test to various concentrations under defined conditions for 48 hours. The recorded effects were the mobility of the daphnids after 24 and 48 hours.

The study encompassed 6 treatment groups each containing 20 individuals. The mobility of the daphnids was determined in a static 48-hour test by visual observation after 24 and 48 hours. The time weighted average concentrations of the test item were 14, 4.96, 2.68, 1.19 and 0.292 mg test item/L. In the control, test water was used without addition of the test item.

After 48 hours of exposure no immobilisation of the test animals was observed only in the control. The 48-hour NOEC was determined to be < 0.292 mg test item/L. The 48-hour LOEC was determined to be ≤ 0.292 mg test item/L and the 48-hour EC50 value was calculated to be 0.367 mg test item/L.