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Short-term toxicity to aquatic invertebrates

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Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Guideline study
Reason / purpose for cross-reference:
reference to other study
Qualifier:
according to guideline
Guideline:
other: ASTM E729-95 Standard Guide for Conducting Acute Toxicity Tests on Test Materials with Fishes, Macroinvertebrates, and Amphibians
Qualifier:
equivalent or similar to guideline
Guideline:
EPA OPPTS 850.1010 (Aquatic Invertebrate Acute Toxicity Test, Freshwater Daphnids)
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
no
Remarks:
No data is available on the GLP compliance
Analytical monitoring:
yes
Details on sampling:
Each treatment was sampled at beginning and end of test
Vehicle:
no
Test organisms (species):
Ceriodaphnia dubia
Details on test organisms:
TEST ORGANISM
- Common name: Ceriodaphnia, water flea
- Source: In-house culture
- Age at study initiation (mean and range, SD): <24 h
- Feeding during test: none
- Food type:
- Acclimation: cultured in dilution water
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Hardness:
90 mg/L
Test temperature:
24.0 °C
pH:
mean = 7.4. control = 7.8, highest boron concentration = 6.8
Dissolved oxygen:
> 6.0 mg/L
Nominal and measured concentrations:
Nominal: Control, 31.3, 62.5, 125, 250, 500 mg-B/L
Measured: 0.64, 31, 62, 125, 245, 490 mg-B/L
Details on test conditions:
TEST SYSTEM
- Test vessel: 50 ml beaker
- Type: open
- Material, size, headspace, fill volume: glass, 40 ml
- Aeration: none
- Renewal rate of test solution: none
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: MHRW (moderately hard reconstituted water, per US EPA 2002)
- Total organic carbon:
- Particulate matter:
- Metals:
- Pesticides:
- Chlorine: 0 nominal
- Ca/mg ratio: 5.40
- Conductivity:
- Salinity:
- Culture medium different from test medium: Cultured in test medium
- Intervals of water quality measurement: boron, pH, conductivity, alkalinity, hardness at beginning and end of test, temperature and D.O. daily

OTHER TEST CONDITIONS
- Adjustment of pH: none
- Photoperiod: 16 L: 8 D

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Mortality (daily)
Duration:
48 h
Dose descriptor:
LC50
Effect conc.:
102 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
element
Basis for effect:
mortality
Remarks on result:
other: (95% CI 82-125 mg-B/L)
Reported statistics and error estimates:
LC50 calculated using trimmed Spearman-Karber method

Data

Concentration (mg B/L) # dead (out of 20) Percent
0.64 (control) 2 10.0%
31 2 10.0%
62 9 45.0%
125 9 45.0%
246 20 100.0%
490 20 100.0%
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
migrated information: read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Guideline study
Reason / purpose for cross-reference:
reference to other study
Qualifier:
according to guideline
Guideline:
other: ASTM E729-95 Standard Guide for Conducting Acute Toxicity Tests on Test Materials with Fishes, Macroinvertebrates, and Amphibians
Qualifier:
equivalent or similar to guideline
Guideline:
EPA OPPTS 850.1010 (Aquatic Invertebrate Acute Toxicity Test, Freshwater Daphnids)
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
no
Analytical monitoring:
yes
Details on sampling:
Each treatment was sampled at beginning and end of test
Vehicle:
no
Test organisms (species):
Ceriodaphnia dubia
Details on test organisms:
TEST ORGANISM
- Common name: Ceriodaphnia, water flea
- Source: In-house culture
- Age at study initiation (mean and range, SD): <24 h
- Feeding during test: none
- Food type:
- Acclimation: cultured in dilution water
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Hardness:
91 mg/L
Test temperature:
24.3 °C
pH:
8.0
Dissolved oxygen:
> 6.0 mg/L
Nominal and measured concentrations:
Nominal: Control, 31.3, 62.5, 125, 250, 500 mg-B/L
Measured: 0.6, 35, 69, 135, 260, 540 mg-B/L
Details on test conditions:
TEST SYSTEM
- Test vessel: 50 ml beaker
- Type: open
- Material, size, headspace, fill volume: glass, 40 ml
- Aeration: none
- Renewal rate of test solution: none
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: MHRW (moderately hard reconstituted water, per US EPA 2002)
- Total organic carbon: 0 nominal
- Particulate matter:
- Metals:
- Pesticides:
- Chlorine: 0 nominal
- Ca/mg ratio: 1.46 (mass:mass)
- Conductivity:
- Salinity:
- Culture medium different from test medium: Cultured in test medium
- Intervals of water quality measurement: boron, pH, conductivity, alkalinity, hardness at beginning and end of test, temperature and D.O. daily

OTHER TEST CONDITIONS
- Adjustment of pH: none
- Photoperiod: 16 L: 8 D

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Mortality (daily)
Duration:
48 h
Dose descriptor:
LC50
Effect conc.:
165 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
element
Basis for effect:
mortality
Remarks on result:
other: (95% CI 137-198 mg-B/L)
Reported statistics and error estimates:
LC50 calculated using trimmed Spearman-Karber method

Data

Concentration (mg B/L) # dead (out of 20) Percent
0.6 (control) 2 10.0%
35 5 25.0%
69 4 20.0%
135 6 30.0%
260 20 100.0%
535 20 100.0%
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
migrated information: read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Guideline study
Reason / purpose for cross-reference:
reference to other study
Qualifier:
according to guideline
Guideline:
other: ASTM E729-95 Standard Guide for Conducting Acute Toxicity Tests on Test Materials with Fishes, Macroinvertebrates, and Amphibians
Qualifier:
equivalent or similar to guideline
Guideline:
EPA OPPTS 850.1010 (Aquatic Invertebrate Acute Toxicity Test, Freshwater Daphnids)
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
no
Analytical monitoring:
yes
Details on sampling:
Each treatment was sampled at beginning and end of test
Vehicle:
no
Test organisms (species):
Ceriodaphnia dubia
Details on test organisms:
TEST ORGANISM
- Common name: Ceriodaphnia, water flea
- Source: In-house culture
- Age at study initiation (mean and range, SD): <24 h
- Feeding during test: none
- Food type:
- Acclimation: cultured in dilution water
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Hardness:
89 mg/L
Test temperature:
25.0 °C
pH:
8.1
Dissolved oxygen:
> 6.0 mg/L
Nominal and measured concentrations:
Nominal: Control, 31.3, 62.5, 125, 250, 500 mg-B/L
Measured: <0.047, 36, 72, 135, 280, 535 mg-B/L
Details on test conditions:
TEST SYSTEM
- Test vessel: 50 ml beaker
- Type: open
- Material, size, headspace, fill volume: glass, 40 ml
- Aeration: none
- Renewal rate of test solution: none
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: MHRW (moderately hard reconstituted water, per US EPA 2002)
- Total organic carbon: 0 nominal
- Particulate matter:
- Metals:
- Pesticides:
- Chlorine: 0 nominal
- Ca/mg ratio: 1.46 (mass:mass)
- Conductivity:
- Salinity:
- Culture medium different from test medium: Cultured in test medium
- Intervals of water quality measurement: boron, pH, conductivity, alkalinity, hardness at beginning and end of test, temperature and D.O. daily

OTHER TEST CONDITIONS
- Adjustment of pH: none
- Photoperiod: 16 L: 8 D

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Mortality (daily)
Duration:
48 h
Dose descriptor:
LC50
Effect conc.:
109 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
element
Basis for effect:
mortality
Remarks on result:
other: (95% CI 93-128 mg-B/L)
Reported statistics and error estimates:
LC50 calculated using trimmed Spearman-Karber method

Data

Concentration (mg B/L) # dead (out of 20) Percent
<0.047 (control) 1 5.0%
36 2 10.0%
72 2 10.0%
135 15 75.0%
280 20 100.0%
535 20 100.0%
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
migrated information: read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Guideline study
Reason / purpose for cross-reference:
reference to other study
Qualifier:
according to guideline
Guideline:
other: ASTM E729-95 Standard Guide for Conducting Acute Toxicity Tests on Test Materials with Fishes, Macroinvertebrates, and Amphibians
Qualifier:
equivalent or similar to guideline
Guideline:
EPA OPPTS 850.1010 (Aquatic Invertebrate Acute Toxicity Test, Freshwater Daphnids)
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
no
Analytical monitoring:
yes
Details on sampling:
Each treatment was sampled at beginning and end of test
Vehicle:
no
Test organisms (species):
Ceriodaphnia dubia
Details on test organisms:
TEST ORGANISM
- Common name: Ceriodaphnia, water flea
- Source: In-house culture
- Age at study initiation (mean and range, SD): <24 h
- Feeding during test: none
- Food type:
- Acclimation: cultured in dilution water
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Hardness:
282 mg/L
Test temperature:
25.0 °C
pH:
8.1
Dissolved oxygen:
> 6.0 mg/L
Nominal and measured concentrations:
Nominal: Control, 31.3, 62.5, 125, 250, 500 mg-B/L
Measured: <0.02, 34, 70, 135, 265, 530 mg-B/L
Details on test conditions:
TEST SYSTEM
- Test vessel: 50 ml beaker
- Type: open
- Material, size, headspace, fill volume: glass, 40 ml
- Aeration: none
- Renewal rate of test solution: none
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: MHRW (moderately hard reconstituted water, per US EPA 2002) modified to make hardness = 300 mg/L (added MgSO4 and CaSO4 in same proportions as standard MHRW)
- Total organic carbon: 0 nominal
- Particulate matter:
- Metals:
- Pesticides:
- Chlorine: 0 nominal
- Ca/mg ratio: 1.46 (mass:mass)
- Conductivity:
- Salinity:
- Culture medium different from test medium: Cultured in test medium
- Intervals of water quality measurement: boron, pH, conductivity, alkalinity, hardness at beginning and end of test, temperature and D.O. daily

OTHER TEST CONDITIONS
- Adjustment of pH: none
- Photoperiod: 16 L: 8 D

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Mortality (daily)
Duration:
48 h
Dose descriptor:
LC50
Effect conc.:
104 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
element
Basis for effect:
mortality
Remarks on result:
other: (95% CI 87-123 mg-B/L)
Reported statistics and error estimates:
LC50 calculated using trimmed Spearman-Karber method

Data

Concentration (mg B/L) # dead (out of 20) Percent
<0.02 (control) 2 10.0%
34 0 0.0%
70 3 15.0%
135 15 75.0%
265 20 100.0%
530 20 100.0%
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
migrated information: read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Guideline study
Reason / purpose for cross-reference:
reference to other study
Qualifier:
according to guideline
Guideline:
other: ASTM E729-95 Standard Guide for Conducting Acute Toxicity Tests on Test Materials with Fishes, Macroinvertebrates, and Amphibians
Qualifier:
equivalent or similar to guideline
Guideline:
EPA OPPTS 850.1010 (Aquatic Invertebrate Acute Toxicity Test, Freshwater Daphnids)
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
no
Analytical monitoring:
yes
Details on sampling:
Each treatment was sampled at beginning and end of test
Vehicle:
no
Test organisms (species):
Ceriodaphnia dubia
Details on test organisms:
TEST ORGANISM
- Common name: Ceriodaphnia, water flea
- Source: In-house culture
- Age at study initiation (mean and range, SD): <24 h
- Feeding during test: none
- Food type:
- Acclimation: cultured in dilution water
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Hardness:
469 mg/L
Test temperature:
25.0 °C
pH:
8.1
Dissolved oxygen:
> 6.0 mg/L
Nominal and measured concentrations:
Nominal: Control, 31.3, 62.5, 125, 250, 500 mg-B/L
Measured: <0.02, 35, 70, 140, 265, 530 mg-B/L
Details on test conditions:
TEST SYSTEM
- Test vessel: 50 ml beaker
- Type: open
- Material, size, headspace, fill volume: glass, 40 ml
- Aeration: none
- Renewal rate of test solution: none
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: MHRW (moderately hard reconstituted water, per US EPA 2002) modified to make hardness = 500 mg/L (added MgSO4 and CaSO4 in same proportions as standard MHRW)
- Total organic carbon: 0 nominal
- Particulate matter:
- Metals:
- Pesticides:
- Chlorine: 0 nominal
- Ca/mg ratio: 1.46 (mass:mass)
- Conductivity:
- Salinity:
- Culture medium different from test medium: Cultured in test medium
- Intervals of water quality measurement: boron, pH, conductivity, alkalinity, hardness at beginning and end of test, temperature and D.O. daily

OTHER TEST CONDITIONS
- Adjustment of pH: none
- Photoperiod: 16 L: 8 D

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Mortality (daily)
Duration:
48 h
Dose descriptor:
LC50
Effect conc.:
93 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
element
Basis for effect:
mortality
Remarks on result:
other: (95% CI 77-114 mg-B/L)
Reported statistics and error estimates:
LC50 calculated using trimmed Spearman-Karber method

Data

Concentration (mg B/L) # dead (out of 20) Percent
<0.02 (control) 2 10.0%
35 2 10.0%
70 4 20.0%
140 17 85.0%
265 20 100.0%
530 20 100.0%
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
migrated information: read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Guideline study
Reason / purpose for cross-reference:
reference to other study
Qualifier:
according to guideline
Guideline:
other: ASTM E729-95 Standard Guide for Conducting Acute Toxicity Tests on Test Materials with Fishes, Macroinvertebrates, and Amphibians
Qualifier:
equivalent or similar to guideline
Guideline:
EPA OPPTS 850.1010 (Aquatic Invertebrate Acute Toxicity Test, Freshwater Daphnids)
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
no
Analytical monitoring:
yes
Details on sampling:
Each treatment was sampled at beginning and end of test
Vehicle:
no
Test organisms (species):
Ceriodaphnia dubia
Details on test organisms:
TEST ORGANISM
- Common name: Ceriodaphnia, water flea
- Source: In-house culture
- Age at study initiation (mean and range, SD): <24 h
- Feeding during test: none
- Food type:
- Acclimation: cultured in dilution water
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Hardness:
85 mg/L
Test temperature:
25.0 °C
pH:
6.7
Dissolved oxygen:
> 6.0 mg/L
Nominal and measured concentrations:
Nominal: Control, 18.75, 37.5, 75, 150, 300 mg-B/L
Measured: 0.03, 21, 44, 84, 170, 335 mg-B/L
Details on test conditions:
TEST SYSTEM
- Test vessel: 50 ml beaker
- Type: open
- Material, size, headspace, fill volume: glass, 40 ml
- Aeration: none
- Renewal rate of test solution: none
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: MHRW (moderately hard reconstituted water, per US EPA 2002) modified to make pH = 6.5 (reduced NaHCO3 to 4 mg/L rather than 96 mg/L like standard MHRW)
- Total organic carbon: 0 nominal
- Particulate matter:
- Metals:
- Pesticides:
- Chlorine: 0 nominal
- Ca/mg ratio: 1.46 (mass:mass)
- Conductivity:
- Salinity:
- Culture medium different from test medium: Cultured in test medium
- Intervals of water quality measurement: boron, pH, conductivity, alkalinity, hardness at beginning and end of test, temperature and D.O. daily

OTHER TEST CONDITIONS
- Adjustment of pH: none
- Photoperiod: 16 L: 8 D

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Mortality (daily)
Duration:
48 h
Dose descriptor:
LC50
Effect conc.:
91 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
element
Basis for effect:
mortality
Remarks on result:
other: (95% CI 79-106 mg-B/L)
Reported statistics and error estimates:
LC50 calculated using trimmed Spearman-Karber method

Data

Concentration (mg B/L) # dead (out of 20) Percent
0.03 (control) 0 0.0%
21 0 0.0%
44 0 0.0%
84 8 40.0%
170 20 100.0%
335 20 100.0%
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
migrated information: read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Guideline study
Reason / purpose for cross-reference:
reference to other study
Qualifier:
according to guideline
Guideline:
other: ASTM E729-95 Standard Guide for Conducting Acute Toxicity Tests on Test Materials with Fishes, Macroinvertebrates, and Amphibians
Qualifier:
equivalent or similar to guideline
Guideline:
EPA OPPTS 850.1010 (Aquatic Invertebrate Acute Toxicity Test, Freshwater Daphnids)
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
no
Analytical monitoring:
yes
Details on sampling:
Each treatment was sampled at beginning and end of test
Vehicle:
no
Test organisms (species):
Ceriodaphnia dubia
Details on test organisms:
TEST ORGANISM
- Common name: Ceriodaphnia, water flea
- Source: In-house culture
- Age at study initiation (mean and range, SD): <24 h
- Feeding during test: none
- Food type:
- Acclimation: cultured in dilution water
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Hardness:
87 mg/L
Test temperature:
24.9 °C
pH:
7.6
Dissolved oxygen:
> 6.0 mg/L
Nominal and measured concentrations:
Nominal: Control, 18.75, 37.5, 75, 150, 300 mg-B/L
Measured: 0.03, 19, 44, 88, 160, 315 mg-B/L
Details on test conditions:
TEST SYSTEM
- Test vessel: 50 ml beaker
- Type: open
- Material, size, headspace, fill volume: glass, 40 ml
- Aeration: none
- Renewal rate of test solution: none
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: MHRW (moderately hard reconstituted water, per US EPA 2002) modified to make pH = 7.5 (reduced NaHCO3 to 39.8 mg/L rather than 96 mg/L like standard MHRW)
- Total organic carbon: 0 nominal
- Particulate matter:
- Metals:
- Pesticides:
- Chlorine: 0 nominal
- Ca/mg ratio: 1.46 (mass:mass)
- Conductivity:
- Salinity:
- Culture medium different from test medium: Cultured in test medium
- Intervals of water quality measurement: boron, pH, conductivity, alkalinity, hardness at beginning and end of test, temperature and D.O. daily

OTHER TEST CONDITIONS
- Adjustment of pH: none
- Photoperiod: 16 L: 8 D

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Mortality (daily)
Duration:
48 h
Dose descriptor:
LC50
Effect conc.:
115 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
element
Basis for effect:
mortality
Remarks on result:
other: (95% CI 108-122 mg-B/L)
Reported statistics and error estimates:
LC50 calculated using trimmed Spearman-Karber method

Data

Concentration (mg B/L) # dead (out of 20) Percent
0.03 (control) 0 0.0%
19 0 0.0%
44 0 0.0%
88 1 5.0%
160 20 100.0%
335 20 100.0%
     
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
migrated information: read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Guideline study
Reason / purpose for cross-reference:
reference to other study
Qualifier:
according to guideline
Guideline:
other: ASTM E729-95 Standard Guide for Conducting Acute Toxicity Tests on Test Materials with Fishes, Macroinvertebrates, and Amphibians
Qualifier:
equivalent or similar to guideline
Guideline:
EPA OPPTS 850.1010 (Aquatic Invertebrate Acute Toxicity Test, Freshwater Daphnids)
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
no
Analytical monitoring:
yes
Details on sampling:
Each treatment was sampled at beginning and end of test
Vehicle:
no
Test organisms (species):
Ceriodaphnia dubia
Details on test organisms:
TEST ORGANISM
- Common name: Ceriodaphnia, water flea
- Source: In-house culture
- Age at study initiation (mean and range, SD): <24 h
- Feeding during test: none
- Food type:
- Acclimation: cultured in dilution water
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Hardness:
84 mg/L
Test temperature:
25.0 °C
pH:
8.4
Dissolved oxygen:
> 6.0 mg/L
Nominal and measured concentrations:
Nominal: Control, 25, 50, 100, 200, 400 mg-B/L
Measured: 0.03, 26, 56, 110, 210, 425 mg-B/L
Details on test conditions:
TEST SYSTEM
- Test vessel: 50 ml beaker
- Type: open
- Material, size, headspace, fill volume: glass, 40 ml
- Aeration: none
- Renewal rate of test solution: none
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: MHRW (moderately hard reconstituted water, per US EPA 2002) modified to make pH = 8.5 (increased NaHCO3 to 398.5 mg/L rather than 96 mg/L like standard MHRW)
- Total organic carbon: 0 nominal
- Particulate matter:
- Metals:
- Pesticides:
- Chlorine: 0 nominal
- Ca/mg ratio: 1.46 (mass:mass)
- Conductivity:
- Salinity:
- Culture medium different from test medium: Cultured in test medium
- Intervals of water quality measurement: boron, pH, conductivity, alkalinity, hardness at beginning and end of test, temperature and D.O. daily

OTHER TEST CONDITIONS
- Adjustment of pH: none
- Photoperiod: 16 L: 8 D

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Mortality (daily)
Duration:
48 h
Dose descriptor:
LC50
Effect conc.:
142 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
element
Basis for effect:
mortality
Remarks on result:
other: (95% CI 130-155 mg-B/L)
Reported statistics and error estimates:
LC50 calculated using trimmed Spearman-Karber method

Data

Concentration (mg B/L) # dead (out of 20) Percent
0.03 (control) 0 0.0%
26 0 0.0%
56 0 0.0%
110 2 10.0%
210 20 100.0%
425 20 100.0%

Description of key information

Short-term effects on freshwater invertebrates ranged from 64 to >544 mg B/L.

Adequate short-term LC50 of 130 mg B/L was found for the marine shrimp L. vannamei.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
64 mg/L

Marine water invertebrates

Marine water invertebrates
Effect concentration:
130 mg/L

Additional information