Triphenylphosphine oxide

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Qualifier:

according to guideline

Guideline:

other: The test follows the EU Directive 79/831/EEC, Annex V, part C

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

no

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: test solution was added without any pretreatment.

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: water flea
- Strain: Straus
- Source: Daphnia magna Straus, originally obtained from the Institute National de Recherche Chimique Appliquee, France and cultured in the laboratories of BASF since 1978.
- Age at study initiation (mean and range, SD): 2-24 hours
- Feeding during test
- Food type: Whilst changing water Brewer's yeast was given, also 1 x daily grown Green algae culture

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Hardness:

2.70 +/- 0.5 mmol/L

Test temperature:

18-20 °C

pH:

about 8.0

Dissolved oxygen:

7.1 - 8.6 mg/L

Nominal and measured concentrations:

The test medium of the highest test concentration, 125 mg/L (nominal) was prepared. Subsequently, serial dilutions of this test medium were prepared in order to obtain the desired test concentrations. A control was tested in parallel.
Nominal concentrations: 7.81, 15.6, 31.2, 62.5, 125 mg/mL

Details on test conditions:

TEST SYSTEM
- Test vessel: flat bottom glass tubes
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: according to the test guideline DIN 38412, L 11
- Culture medium different from test medium: Not specified

OTHER TEST CONDITIONS
- Photoperiod: 16 : 8 hours day-night regime
- Light intensity: diffuse light (5 microeinstein/m*m*s) at 400 - 750 nm wave length

EFFECT PARAMETERS MEASURED: mobility; visually after 0, 3, 6, 24, 48 h

Reference substance (positive control):

no

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

31.2 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

42.7 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

62.5 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Reported statistics and error estimates:

The EC50 (24-h and 48-h) was determined by the moving avrage method. The EC0 was determined by the highest concentration at which <= 10% immobilisation occurred. The EC0 was determined by the lowest concentration at which 100 % immobilisation occurred.

Table: Mobile daphnids after 0, 24 and 48 hours of exposure

Concentration [mg/L]	Mobile test animals after
	0 h	24 h	48 h
7.01	20	20	20
15.6	20	20	20
31.2	20	20	19
62.5	20	5	0
125	20	0	0
0	20	20	20

Validity criteria fulfilled:

not specified

Conclusions:

In an aquatic test with Daphnia magna according to EU Directive 79/831/EEC, Annex V, part C and OECD Guideline 202 the 48-hour EC50 of the test item was determined to be 42.7 mg/L (nominal).

Executive summary:

The toxicity of the test item to aquatic invertebrates was assessed in a static, 48-hour test according to EU Directive 79/831/EEC, Annex V, part C and OECD Guideline 202. Young daphnids (2 - 24 hours old; 5 animals per test vessel) were exposed to nominal test item concentrations of: 7.81, 15.6, 31.2, 62.5, 125 mg/mL (4 replicates per concentration). A negative control (dilution water only; five replicates) was tested in parallel. The solution water used for the test was prepared according to the test guideline DIN 38412, L 11. Daphnids were fed during the test with Brewer's yeast and green algae (daily). The light regime was 16 light : 8 darkness at a test temperature of 18-20 °C.  Mobility of the test animals was checked visually after 0, 3, 6, 24 and 48 hours of exposure. In result, the 48-hour EC0 and EC100 of the test item was determined to be 31.2 mg/L and 62.5 mg/L in nominal, respectively. The 48-hour EC50 of the test item was 42.7 mg/L (nominal).

Description of key information

In a test with Daphnia magna according to EU Directive 79/831/EEC, Annex V, part C and OECD Guideline 202, the 48-hour EC50 of the test item was determined to be 42.7 mg/L (nominal).

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

42.7 mg/L

Additional information

The toxicity of the test item to aquatic invertebrates was assessed in a static, 48-hour test according to EU Directive 79/831/EEC, Annex V, part C and OECD Guideline 202. Young daphnids (2 - 24 hours old; 5 animals per test vessel) were exposed to nominal test item concentrations of: 7.81, 15.6, 31.2, 62.5, 125 mg/mL (4 replicates per concentration). A negative control (dilution water only; five replicates) was tested in parallel. The solution water used for the test was prepared according to the test guideline DIN 38412, L 11. Daphnids were fed during the test with Brewer's yeast and green algae (daily). The light regime was 16 light : 8 darkness at a test temperature of 18-20 °C.  Mobility of the test animals was checked visually after 0, 3, 6, 24 and 48 hours of exposure. In result, the 48-hour EC0 and EC100 of the test item was determined to be 31.2 mg/L and 62.5 mg/L in nominal, respectively. The 48-hour EC50 of the test item was 42.7 mg/L (nominal).