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Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Study period:
24 Aug - 27 Aug, 1999
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Done under GLP and OECD methods

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1999
Report Date:
1999

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
EU Method C.3 (Algal Inhibition test)
Version / remarks:
Method C3, Directive 92/69/EEC
GLP compliance:
yes
Remarks:
OECD 30

Test material

Reference
Name:
Unnamed
Type:
Constituent

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
no
Details on test solutions:
Nominal test concentrations: 10, 18, 32, 56 and 100mg/L
Mean measured test concentrations at t=0: 9.82, 17.68, 32.02, 55.33, and 97.17mg/L
Mean measured test concentrations at t=72hrs: 10.42, 17.66, 32.63, 55.89 and 98.13mg/L

Test organisms

Test organisms (species):
Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)
Details on test organisms:
TEST ORGANISM
- Strain: CCAP 278/4
- Source (laboratory, culture collection): Culture Centre of Algae and Protozoa, Institute of FreshWater Ecology, Windermere Laboratory, Far Sawrey, Ambleside, Cumbria LA22 0LP, England.

Study design

Test type:
other: aeration
Limit test:
no
Total exposure duration:
72 h

Test conditions

Test temperature:
23 ± 2°C
Nominal and measured concentrations:
Nominal test concentrations: 10, 18, 32, 56 and 100mg/L
Mean measured test concentrations at t=0: 9.82, 17.68, 32.02, 55.33, and 97.17mg/L
Mean measured test concentrations at t=72hrs: 10.42, 17.66, 32.63, 55.89 and 98.13mg/L
Details on test conditions:
Algae were cultured for 72hrs under continuous illumination of approx. 6,000 lux in the spectral range 400 -700 nm.

Results and discussion

Effect concentrationsopen allclose all
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
<= 87.2 mg/L
Nominal / measured:
meas. (not specified)
Basis for effect:
biomass
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
<= 100 mg/L
Nominal / measured:
meas. (not specified)
Basis for effect:
growth rate
Duration:
72 h
Dose descriptor:
NOEC
Effect conc.:
<= 100 mg/L
Nominal / measured:
meas. (not specified)
Basis for effect:
growth rate
Duration:
72 h
Dose descriptor:
NOEC
Effect conc.:
< 100 mg/L
Nominal / measured:
meas. (not specified)
Basis for effect:
biomass
Details on results:
ECr50(24h) : >100mg/L measured
ECr50(48h) : >100mg/L measured
ECr50(72h) : >100mg/L measured

ECb50(24h) : 74.48mg/L measured (95% confidence limits: 61.28 - 111.48)
ECb50(48h) : 64.17mg/L measured (95% confidence limits: 51.30 - 113.33)
ECb50(72h) : 87.18mg/L measured (95% confidence limits: 60.49 - 359.25)

NOEC b (24hr): 56mg/L measured (by ANOVA)
NOEC b (48hr): 10mg/L measured (by ANOVA)
NOEC b (72hr): <10mg/L measured (by ANOVA)
NOEC r (24hr): 56mg/L measured (by ANOVA)
NOEC r (48hr): 18mg/L measured (by ANOVA)
NOEC r (72hr): <100mg/L measured (by ANOVA)

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
ECr50(24h) : >100mg/L measured
ECr50(48h) : >100mg/L measured
ECr50(72h) : >100mg/L measured

ECb50(24h) : 74.48mg/L measured (95% confidence limits: 61.28 - 111.48)
ECb50(48h) : 64.17mg/L measured (95% confidence limits: 51.30 - 113.33)
ECb50(72h) : 87.18mg/L measured (95% confidence limits: 60.49 - 359.25)

NOEC b (24hr): 56mg/L measured (by ANOVA)
NOEC b (48hr): 10mg/L measured (by ANOVA)
NOEC b (72hr): <10mg/L measured (by ANOVA)
NOEC r (24hr): 56mg/L measured (by ANOVA)
NOEC r (48hr): 18mg/L measured (by ANOVA)
NOEC r (72hr): <100mg/L measured (by ANOVA)