Amides, C16-18 (even numbered) saturated and C18 unsaturated, N,N'-[[(2-hydroxyethyl)imino]di-2,1-ethanediyl]bis-, ethoxylated (1-2.5 mol)

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1990

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

GLP compliance:

yes

Specific details on test material used for the study:

V 2870
Batch No. Eg 203

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

synthetic water prepared according to OECD guideline 202

Test organisms (species):

Daphnia magna

Details on test organisms:

laboratory breed
age of daphnia. 6 - 24 h

Holding conditions:
Temperature 18°C - 20°C
O2-Concentration at least 80 % of air saturation
pH-Value: 7.0 - 8.5
Hardness: 14° dH
Light 16 h daily
Feeding. once daily

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test temperature:

18°C - 20°C

pH:

between 8.44 and 4.57 at 48 h

Dissolved oxygen:

> 60 % of air saturation

Nominal and measured concentrations:

Nominal preliminary test: 100, 10, 1 g/L, 100, 10, 1, 0.1 mg/L
Nominal main test: 100, 71, 51, 36, 26, 19, 14, 10 g/L

Details on test conditions:

TEST SYSTEM
- Test vessel: 25 ml volumetric cylinder

- Aeration: O2-concentration > 60 % of air saturation

- No. of organisms per vessel:5
- No. of vessels per concentration (replicates):4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: synthetic water


OTHER TEST CONDITIONS
- Photoperiod: 16 h daily


VEHICLE CONTROL PERFORMED: yes

RANGE-FINDING STUDY
- Test concentrations: 100, 10, 1 g/L, 100, 10, 1, 0.1 mg/L
- Results used to determine the conditions for the definitive study: yes

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

18.62 g/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Results of the main test: The number of living daphnia (sum) and the rate of immobilisation (%) after 48 h is given.

concentration.g/L	100	71	51	36	26	19	14	10	control
group I	0	0	0	0	3	3	3	4	5
group II	0	0	0	0	1	1	4	4	5
group III	0	0	0	0	2	5	3	4	5
group IV	0	0	0	3	0	3	1	5	5
sum	0	0	0	3	6	12	11	17	20
percentage	100	100	100	85	70	40	45	15	0

Validity criteria fulfilled:

not specified

Conclusions:

The 48 h LC0 was not determinable for the main test. The 48 h EC50 was at 18.62.g/L of the formulation "V2870".

Description of key information

The 48 h EC50 was at 18.62 g/L.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

18.62 g/L