Methanone, 1,1'-(1,4-phenylene)bis[1-(4-phenoxyphenyl)-

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Justification for type of information:

Experimental test result performed according to the guideline.

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Principles of method if other than guideline:

Acute Immobilization Study of 1,4-phenylene bis[(4-phenoxyphenyl)-methanone] on Daphnia magna in a Semi-Static System for 48 hrs. Test performed according to the OECD guideline 202.

GLP compliance:

no

Specific details on test material used for the study:

- Name of test material (IUPAC name): 1,4-phenylene bis[(4-phenoxyphenyl)-methanone]
- Molecular formula: C32H22O4
- Molecular weight: 470.5218 g/mol
- Smiles : c1ccc(cc1)Oc2ccc(cc2)C(=O)c3ccc(cc3)C(=O)c4ccc(cc4)Oc5ccccc5
- InChI : 1S/C32H22O4/c33-31(25-15-19-29(20-16-25)35-27-7-3-1-4-8-27)23-11-13-24(14-12-23)32(34)26-17-21-30(22-18-26)36-28-9-5-2-6-10-28/h1-22H
- Substance Type: Organic
- Physical State: Solid

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

The test substance was poorly soluble in water. Therefore, the test solution was prepared by dissolving 500 mg of the test substance in 500 ml of ADaM’s media. Kept it for 72 hrs stirring, after stirring stock was filtered and analytically detected and the stock concentration was found to be 0.2046 mg/L.As it is stock taken and set up limit test on this specific value.

Test organisms (species):

Daphnia magna

Details on test organisms:

- Common name: Water flea
- Source: Own breeding of daphnia magna
- Food: No feeding after hatching

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Post exposure observation period:

24 and 48 hrs

Hardness:

144.5 mg of CaCO3)

Test temperature:

20±2 °C

pH:

7.2

Nominal and measured concentrations:

0.2046 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: Glass beaker
- Aeration: No aeration during experiment
- Material, size, headspace, fill volume: 25 ml of glass beaker filled with 20 ml media having headspace of 5 ml
- No. of organisms per vessel: 10 daphnids

OTHER TEST CONDITIONS
- Photoperiod:16 hours light and 8 hours dark

Reference substance (positive control):

no

Key result

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

0.205 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: No effects were observed

Table 1: Immobility

Nominal Concentration (mg/l)	Number of Daphnids	Immobility
		0 hrs	24 hrs	48 hrs
0.2046	10	-	-		-
0.2046	10	-	-		-
Any Abnormal Behaviour(if any)		No observed effects	No observed effects		No observed effects

Table 2: pH and oxygen content

Nominal Concentration (mg/l)	Number of Daphnids	pH value
		0 hrs	48 hrs
control	10	7.4	7.6	7.8
0.2046	10	7.5	7.7	7.9
0.2046	10	7.5	7.7	7.9

Nominal Concentration (mg/l)	Number of Daphnids	DO(mg/L)
		0 hrs	48 hrs
Control	10	6.0	5.8	5.8
0.2046	10	6.1	5.9	5.9
0.2046	10	6.1	5.9	5.9

Table 3: Test temperature

Nominal Concentration (mg/l)	Number of Daphnids	Temperature
		0 hrs	48 hrs
Control	10	20.1		20.1
0.2046	10	20.1		20.1
0.2046	10	20.1		20.1

Validity criteria fulfilled:

yes

Remarks:

1. In the control,0 percent of the daphnids observed immobilized. 2. The dissolved oxygen concentration at the end of the test should be 5.8 mg/l in control and 5.9 in test vessels

Conclusions:

After the exposure of chemical with daphnia magna for period of 48 hrs, no effects were observed on the mobility of daphnia magna. Thus the EC0 was determine to be 0.2046 mg/l.

Executive summary:

Daphnia sp., Acute Immobilization Test according to OECD Guideline 203 was conducted for 1,4-phenylene bis[(4-phenoxyphenyl)-methanone]. The test substance was poorly soluble in water. Therefore, the test solution was prepared by dissolving 500 mg of the test substance in 500 ml of ADaM’s media. Kept it for 72 hrs stirring, after stirring stock was filtered and analytically detected and the stock concentration was found to be 0.2046 mg/L.As it is stock taken and set up limit test on this specific value. Limit test were performed and test Daphnia magna were exposed to these concentration for 48 hours. Test considers to be valid as it fulfills the validity criteria. In the control, 0 percent of the Daphnis observed immobilized. The dissolved oxygen concentration at the end of the test should be 5.8 mg/l in control and 5.9 in test vessel. After the exposure of chemical with daphnia magna for period of 48 hrs, no effects were observed on the mobility of daphnia magna. Thus the EC0 was determining to be 0.2046 mg/l. As no effects were observed on the mobility of daphnia magna, this chemical considers to be nontoxic and not classified as per the CLP classification criteria.

Description of key information

Daphnia sp., Acute Immobilization Test according to OECD Guideline 203 was conducted for 1,4-phenylene bis[(4-phenoxyphenyl)-methanone]. The test substance was poorly soluble in water. Therefore, the test solution was prepared by dissolving 500 mg of the test substance in 500 ml of ADaM’s media. Kept it for 72 hrs stirring, after stirring stock was filtered and analytically detected and the stock concentration was found to be 0.2046 mg/L.As it is stock taken and set up limit test on this specific value. Limit test were performed and test Daphnia magna were exposed to these concentration for 48 hours. Test considers to be valid as it fulfills the validity criteria. In the control, 0 percent of the Daphnis observed immobilized. The dissolved oxygen concentration at the end of the test should be 5.8 mg/l in control and 5.9 in test vessel. After the exposure of chemical with daphnia magna for period of 48 hrs, no effects were observed on the mobility of daphnia magna. Thus the EC0 was determining to be 0.2046 mg/l. As no effects were observed on the mobility of daphnia magna, this chemical considers to be nontoxic and not classified as per the CLP classification criteria.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

0.205 mg/L

Additional information

Daphnia sp., Acute Immobilization Test according to OECD Guideline 203 was conducted for 1,4-phenylene bis[(4-phenoxyphenyl)-methanone]. The test substance was poorly soluble in water. Therefore, the test solution was prepared by dissolving 500 mg of the test substance in 500 ml of ADaM’s media. Kept it for 72 hrs stirring, after stirring stock was filtered and analytically detected and the stock concentration was found to be 0.2046 mg/L.As it is stock taken and set up limit test on this specific value. Limit test were performed and test Daphnia magna were exposed to these concentration for 48 hours. Test considers to be valid as it fulfills the validity criteria. In the control, 0 percent of the Daphnis observed immobilized. The dissolved oxygen concentration at the end of the test should be 5.8 mg/l in control and 5.9 in test vessel. After the exposure of chemical with daphnia magna for period of 48 hrs, no effects were observed on the mobility of daphnia magna. Thus the EC0 was determining to be 0.2046 mg/l. As no effects were observed on the mobility of daphnia magna, this chemical considers to be nontoxic and not classified as per the CLP classification criteria.