Linseed oil, ester with pentaerythritol

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Study period:

From June, 1988 to July, 1988

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

test procedure in accordance with generally accepted scientific standards and described in sufficient detail

Qualifier:

according to guideline

Guideline:

other: Robra K.H. (1976). Wasser/Abwasser 117:80-86

Deviations:

not specified

Principles of method if other than guideline:

Bacteriotoxic substances inhibit the bacteria's enzymatic activity, leading to decrease oxygen consumption. The Robra-test measures oxygen consumption of Pseudomonas putida cultures exposed to various concentrations of a test substance and the EC10 or EC50 compared to a negative controlare determined.

GLP compliance:

not specified

Analytical monitoring:

not specified

Vehicle:

yes

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): Laurylmyritylalcoholethoxylate 7.5 E.O

Test organisms (species):

Pseudomonas putida

Details on inoculum:

- Laboratory culture: Pseudomonas DSM putida 50026
- Method of cultivation: in standard nutrient solution
- Preparation of inoculum for exposure: Pre-inoculum cultivated at 1°C for ca. 16 h on a rotary shaker. 10 ml pre-cuture taken and dissolved in 200 ml nutrient solution, shaken at 26°C for ca. 16 h prior to test start.

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

30 min

Remarks on exposure duration:

none

Nominal and measured concentrations:

Nominal concentrations: 0, 40,000, 50,000, 60,000, 70,000, 80,000, 90,000 and 100,000 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): closed
- Material, size, headspace, fill volume: 300 ml Erlenmeyer
- Aeration: with pressurized air

Reference substance (positive control):

no

Key result

Duration:

30 min

Dose descriptor:

EC10

Effect conc.:

67 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Validity criteria fulfilled:

not specified

Conclusions:

Under the study conditions, the EC10 to Pseudomonas putida was estimated to be 67000 mg/L.

Executive summary:

A study was conducted to evaluate the toxicity of glycerides, C16-18 and C18-unsatd. (alkali-refined linseed oil) to Pseudomonas putida according to the method described in Robra KH (1976), GFW Wasser/Abwasser 117:80 -86. Microorganisms were exposed to test substance concentrations ranging from 40000 to 100000 mg/L and oxygen consumption was measured. Under the study conditions, the EC10 to Pseudomonas putida was estimated to be 67000 mg/L (Kolvenbach, 1988).

Reference 2

Endpoint:

activated sludge respiration inhibition testing

Data waiving:

study scientifically not necessary / other information available

Justification for data waiving:

the study does not need to be conducted because the substance is found to be readily biodegradable and the applied test concentrations are in the range of concentrations that can be expected in the influent of a sewage treatment plant

Reason / purpose for cross-reference:

data waiving: supporting information

Key value for chemical safety assessment

EC10 or NOEC for microorganisms:

22.8 mg/L

Additional information

Toxicity to aquatic microorganisms was assessed based on studies for substances representative of the main constituents, which can be categorised as pentaerythritol esters (PE) and glycerol esters (GE). The results are presented below:

 

Pentaerythritol esters (PE)

The study does not need to be conducted because the substance, Fatty acids, C14 -18 and C18 -unsatd., branched and linear, esters with pentaerythritol were readily biodegradable in an OECD Guideline 301B study (76.5% degradation after 28 d). The applied test concentration in this study were in the range of those that can expected for the test substance in the influent of a sewage treatment plant. Therefore, the NOEC for toxicity to microorganisms can be considered to be at 22.8 mg/L

 

Glycerol esters (GE)

A study was conducted to evaluate the toxicity of glycerides, C16-18 and C18-unsatd. (alkali-refined linseed oil) to Pseudomonas putida according to the method described in Robra KH (1976), GFW Wasser/Abwasser 117:80 -86. Microorganisms were exposed to test substance concentrations ranging from 40000 to 100000 mg/L and oxygen consumption was measured. Under the study conditions, the EC10 to Pseudomonas putida was estimated to be 67000 mg/L (Kolvenbach, 1988).