Registration Dossier

Diss Factsheets

Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:
toxicity to aquatic algae and cyanobacteria
Remarks:
Classification based on calculation rules for mixtures of the CLP Regulation.
Type of information:
calculation (if not (Q)SAR)
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
accepted calculation method
Justification for type of information:
Classification based on calculation rules for mixtures of the CLP Regulation, based on constituents approach of the UVCB substance marjoram oil.There is no experimental study reference.
Data are compiled on 8 major components which represent about 85% of the UVCB substance.

Data source

Reference
Reference Type:
publication
Title:
ECOSAR v1.11
Author:
EPA United States Environnement Protection Agency
Year:
2012
Bibliographic source:
https://www.epa.gov/tsca-screening-tools/ecological-structure-activity-relationships-ecosar-predictive-model

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: ECOSAR
Version / remarks:
v1.11
Principles of method if other than guideline:
ECOSAR

Test material

Constituent 1
Reference substance name:
Thyme, Thymus mastichina, ext.
EC Number:
284-294-8
EC Name:
Thyme, Thymus mastichina, ext.
Cas Number:
84837-14-9
Molecular formula:
Not relevant for UVCB
IUPAC Name:
Thyme, Thymus mastichina, ext.
Test material form:
liquid: volatile

Results and discussion

Effect concentrations
Duration:
96 h
Dose descriptor:
EC50
Effect conc.:
3.205 mg/L
Details on results:
The toxicity to green algae were estimated using the following additivity formulas, as recommended in the Regulation (EC) No 1272/2008 (CLP) section 4.1.3.5.2:
∑ Ci / L(E)C50m = ∑ (Ci / L(E)C50i)
Where,Ci = concentration of component i (weight percentage);
L(E)C50i = LC50 or EC50 for component i, in mg/L; L(E)C50m = L(E)C50 of the part of the mixture with test data.

Applicant's summary and conclusion

Validity criteria fulfilled:
not applicable
Conclusions:
Based on a weight of evidence approach, the toxicity of assessed substance to green algae during 96 hours can be estimated using adequate toxicity data of major constituents, greater than 1% in the mixture.
These 8 major constituents were considered, representing about 85% of the mixture.
These data are summarized in the above table.
The toxicity to daphnia were estimated using the following additivity formulas, as recommended in the Regulation (EC) No 1272/2008 (CLP) section 4.1.3.5.2:
∑ Ci / L(E)C50m = ∑ (Ci / L(E)C50i)
Where,Ci = concentration of component i (weight percentage);
L(E)C50i = LC50 or EC50 for component i, in mg/L; L(E)C50m = L(E)C50 of the part of the mixture with test data.
Based on data presented above, the EC50 value 96 hours for UVCB substance is calculated at 3.205 mg/L for green Algae .
Executive summary:

EC50 green algae value (96h) for majoram oil is 3.205 mg/l.