Reaction mass of 5-[[4-[benzylmethylamino]phenyl]azo]-1,4-dimethyl-1H-1,2,4-triazolium methyl sulphate and 3-[[4-[benzylmethylamino]phenyl]azo]-1,4-dimethyl-1H-1,2,4-triazolium methyl sulphate

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1980

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Justification for type of information:

None

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

GLP compliance:

no

Specific details on test material used for the study:

None

Analytical monitoring:

no

Vehicle:

no

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Details on test organisms:

None

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test temperature:

16 °C

pH:

7.9 - 8.0

Dissolved oxygen:

7 - 9

Nominal and measured concentrations:

10, 30 and 100 mg/l

Details on test conditions:

None

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

29 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Sublethal observations / clinical signs:

Observations:

Concentration	Mortality				pH	O2
[mg/l]	24 h	48 h	72 h	96 h
10	0/8	0/8	0/8	0/8	7.9	-
30	0/8	1/8	3/7	0/4	8	7
100	8/8	-	-	-	8	9

Validity criteria fulfilled:

no

Conclusions:

96-hour LC50 for FAT 31016/F was determined to be 29 mg/l.

Executive summary:

FAT 31016/F was evaluated for short term toxicity to fish in a study conducted using a methodology similar to OECD Guideline 203. In this study, 8 rainbow trouts per group were exposed to the test substance for 96 hours. 0, 50 and 100% mortality was observed at concentrations of 10, 30 and 100 mg/l, respectively. Hence, based on these findings, 96-hour LC50 for FAT 31016/F was determined to be 29 mg/l.

Reference 2

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1980

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Justification for type of information:

None

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

GLP compliance:

no

Specific details on test material used for the study:

None

Analytical monitoring:

no

Vehicle:

no

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

None

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test temperature:

25 °C

pH:

8.1 - 8.2

Dissolved oxygen:

6 - 6.8

Salinity:

no data available

Conductivity:

no data available

Nominal and measured concentrations:

30 and 100 mg/l

Details on test conditions:

None

Reference substance (positive control):

no

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

46 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Sublethal observations / clinical signs:

Observations:

Concentration	Mortality				pH	O2
[mg/l]	24 h	48 h	72 h	96 h
30	0/10	0/10	0/10	0/10	8.2	6.8
100	10/10	-	-	-	8.1	6

Validity criteria fulfilled:

not specified

Conclusions:

96-hour LC50 for FAT 31016/F was determined to be 46 mg/l.

Executive summary:

FAT 31016/F was evaluated for short term toxicity to fish in a study conducted using a methodology similar to OECD Guideline 203. In this study, 10 rainbow trouts per group were exposed to the test substance for 96 hours. 0 and 100 % mortality was observed at concentrations of 30 and 100 mg/l, respectively. Hence, based on these findings, 96-hour LC50 for FAT 31016/F was determined to be 46 mg/l.

Description of key information

The 96-hour LC50 for FAT 31016/F was determined to be 29 mg/L for rainbow trouts and 46 mg/L for zebra fish.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

29 mg/L

Additional information

FAT 31016/T TE was evaluated for short term toxicity potential to fish in two different species i.e. rainbow trout and zebrafish. The methodology used in the study was similar to OECD Guideline 203, i.e. groups of fish were exposed to different concentrations of the test substance and observations for mortality were used for determination of LC50. From the findings of this study, the 96-hour LC50 for FAT 31016/F was determined to be 29 mg/L for rainbow trouts and 46 mg/L for zebra fish.