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Ecotoxicological information

Toxicity to soil macroorganisms except arthropods

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Endpoint:
toxicity to soil macroorganisms except arthropods: short-term
Type of information:
experimental study
Adequacy of study:
key study
Study period:
no data available
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment
Qualifier:
according to guideline
Guideline:
OECD Guideline 207 (Earthworm, Acute Toxicity Tests)
Version / remarks:
1984
GLP compliance:
no
Remarks:
(Published) Study not performed for REACH purposes. Therefore, GLP-compliance was not an issue.
Analytical monitoring:
not specified
Details on sampling:
no data
Vehicle:
no
Details on preparation and application of test substrate:
EDTA was applied in deionized water solution into artificial soil (35 % dry weight moisture content).
Test organisms (species):
Eisenia fetida
Animal group:
annelids
Details on test organisms:
no data available
Study type:
laboratory study
Substrate type:
artificial soil
Limit test:
no
Total exposure duration:
14 d
Post exposure observation period:
no data available
Test temperature:
20 °C
pH:
no data available
Moisture:
35 % of maximum holding capacity
Details on test conditions:
Glass jars were used as test containers. 750 g of artificial soil which had been treated with one of the test chemicals (or a control with no chemicals) were placed in each jar. Ten E. fetida each with live weights of approximately 1 mg were added to each jar. There were four replicates of each chemical and dose.
Nominal and measured concentrations:
0, 0.1, 1.0, 10, 100, 1000 and 10000 mg/kg dry weight soil.
Reference substance (positive control):
not specified
Key result
Duration:
14 d
Dose descriptor:
LC50
Effect conc.:
156.46 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: 95% CI: 136.01 - 179.98 mg/kg soil dw
Results with reference substance (positive control):
no data available
Reported statistics and error estimates:
Data were analyzed statistically by analysis of variance using S.A.S. software.
The LD50 values were estimated using Trimmed Spearman–Karber Version 1.5 (S.E.P.A. Cincinnati, Ohio) designed for analysis of mortality data from acute chronic toxicity tests.
Validity criteria fulfilled:
not specified
Conclusions:
The 14 day LC50 of EDTA to earthworms (Eisenia foetida) was estimated to be 156.46 mg/kg dry weight soil.
Executive summary:

The 14 day LC50 of EDTA to earthworms (Eisenia foetida) was estimated to be 156.46 mg/kg dry weight soil.

Endpoint:
toxicity to soil macroorganisms except arthropods: short-term
Type of information:
read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
key study
Justification for type of information:
Refer to the Analogue Approach Justification document provided in Section 13.
Reason / purpose for cross-reference:
read-across source
Key result
Duration:
14 d
Dose descriptor:
EC50
Effect conc.:
156.46 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: 95% CI: 136.01 - 179.98 mg/kg soil dw

Description of key information

LC50 (14 d) of 156.46 mg/kg soil dw (OECD 207)

Key value for chemical safety assessment

Additional information

Since no studies investigating the toxicity trisodium hydrogen EDTA (CAS 150-38-9) to soil macroorganisms are available, in accordance to Regulation (EC) No 1907/2006 Annex XI, 1.5 a read-across to structurally related EDTA species was conducted. This read-across is justified in detail in the overall summary (IUCLID Section 6.3) and within the analogue justification in IUCLID Section 13.

For the assessment of toxicity to terrestrial organisms data from the source substance edetic acid (CAS 60-00-4) were used.

The available study was performed according to OECD 207 (Edwards et al., 2009). Eisenia fetida was exposed to EDTA in an artificial soil. Ten species were added to each test vessel and four replicates were prepared for each concentration. Concentrations of 0.1, 1.0, 10.0, 100.0, 1000.0 and 10000.0 mg/kg dw were tested including a control. After 14 d the mortality at the different test vessels was recorded and a LC50 (14 d) of 156.46 mg/kg soil dw resulted.