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EC number: 946-283-2 | CAS number: 1864727-96-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From 6 October, 2017 to 19 December, 2017
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 017
- Report date:
- 2017
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- (cyclohex-3-en-1-yl)methyl 2-hydroxypropanoate
- EC Number:
- 946-283-2
- Cas Number:
- 1864727-96-7
- Molecular formula:
- C10H16O3
- IUPAC Name:
- (cyclohex-3-en-1-yl)methyl 2-hydroxypropanoate
Constituent 1
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: S17-6-100
- Expiration date of the lot/batch: August 7,2018
- Purity test date: 99.4%
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: During the study, the test substance was stored as follows:
Storage condition: room temperature, dark, air-tight, nitrogen-sealed
Storage area: desiccator
- Stability under test conditions: After the exposure period, the infrared absorption spectrum of the test substance was measured. The spectrum obtained was consistent with that measured before the study, indicating that the test substance was stable under the storage conditions.
- Solubility and stability of the test substance in the solvent/vehicle: Water; >100 mg/L, Dimethylsulfoxide; >50 g/L; stable
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: 100 mg/L and control (All test levels)
- Sampling method: (i) At the time of preparation: Collected from the middle layer of the vessel for measuring the test conditions of each test group
(ii) At the end of exposure: Collected from the middle layer of one vessel of each test group
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Dilution water was aerated sufficiently.
1. The test substance was collected in conformity with the following table and the dilution water was added up to final volume.
2.The above solution was stirred for 5 minutes.
- Eluate:Elendt M4 medium
- Controls:The test solution of Control was only dilution water.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna
- Source: National Institute for Environmental Studies, Japan
- Age of parental stock (mean and range, SD): 28 days or less
ACCLIMATION
- Acclimation period: 2-4weeks (Culture period: September 19, 2017 to October 11, 2017)
- Type and amount of food: Chlorella vulgaris (unicellular green algae), 6 mgC/2 L/day
- Health during acclimation (any mortality observed):20% or less during the 2-week period prior to the start of exposure
METHOD FOR PREPARATION AND COLLECTION OF EARLY INSTARS OR OTHER LIFE STAGES: Removed on the day offspring were produced
Study design
- Test type:
- static
- Water media type:
- freshwater
- Remarks:
- Elendt M4 medium
- Limit test:
- yes
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- 242 mg CaCO3/L
- Test temperature:
- 20.0-20.1
- pH:
- 7.3-8.0
- Dissolved oxygen:
- 7.5-8.7
- Details on test conditions:
- TEST SYSTEM
- Test vessel:100-mL glass beaker
- Type (delete if not applicable): closed (cover: Teflon sheet)
- Aeration: No aeration
- Renewal rate of test solution (frequency/flow rate):None (static)
- No. of organisms per vessel:5
- No. of vessels per concentration (replicates):4
- No. of vessels per control (replicates):4
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: prepared Elendt M4 medium
- Culture medium different from test medium: No difference
OTHER TEST CONDITIONS
- Adjustment of pH: No adjustment
- Photoperiod:16-hour light / 8-hour dark
- Light intensity: 800 lux or less
VEHICLE CONTROL PERFORMED: None
RANGE-FINDING STUDY
- Test concentrations: 100, 10, 1.0 and 0.10 mg/L
- Results used to determine the conditions for the definitive study: there was no immobility during the concentrations. The test substance concentration was stable. - Reference substance (positive control):
- yes
- Remarks:
- Acute immobilisation tests of the reference substance (potassium dichromate, guaranteed reagent) have been conducted periodically (approximately every 6 months).
Results and discussion
Effect concentrations
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 91.9 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- - Behavioural abnormalities: It was not observed
- Observations on body length and weight: Nothing
- Other biological observations: Nothing
- Mortality of control: 0%
- Other adverse effects control: None
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: None
- Effect concentrations exceeding solubility of substance in test medium: None - Results with reference substance (positive control):
- - Dose-response test: Yes (periodically)
- ECx: EC50=0.64 mg/L(95% confidence limit:0.56-0.74 mg/L)
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- 48-hour median effect concentration (EC50): >91.9 mg/L (95% confidence limits: not available)
- Executive summary:
The result given below was calculated from the time-weighted mean of measured concentrations. The median effect concentration (EC50) for immobilisation is mentioned as [>test concentration] because this test was limit test at the maximum concentration given in the Test Guideline,and the immobility was less than 50% at this concentration.
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