Reaction Mass of Dialuminium Titanium Pentoxide and Strontium Calcium Aluminosilicate

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

08/04/2011 - 09/06/2011

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Study carried out in accordance with OECD guideline 203 with analytical confirmation of exposure concentrations, GLP standards maintained throughout.

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

Qualifier:

according to guideline

Guideline:

other: other guideline

Version / remarks:

Test method relating to new chemical substances "Alga growth inhibition test, Daphnia sp. acute immobilisation test, and fish acute toxicity test" (Japanese notification, Yakushokuhatsu 0331 No.7, Heisei 23.03.29 Seikyoku No.5, Kanpokihatsu No. 110331009)

Deviations:

no

GLP compliance:

yes

Specific details on test material used for the study:

IUPAC name: Aluminium titanium oxide
CAS No.: 12004-39-6
Molecular weight: 181.83
Purity: 99.9%
Melting point: 1860oC
Solubility in water: <50 mg/mL
Appearance: White powder
Stability: Stable for 5 years at room temperature

Analytical monitoring:

yes

Details on sampling:

Samples of test solutions were taken at the beginning and end of the first and last renewal periods. Two sets of freshly prepared (0 and 72h) and expired (24 and 96h) test solutions were sampled for analysis.

Vehicle:

no

Details on test solutions:

Test solutions were prepared by adding the test material to dilution water (dechlorinated and charcoal-filtered tap water) at a nominal loading rate of 100 mg/L, followed by ultrasonication for 30 minutes, stirring for 48 hours, then filtering (Millex HA 0.45um, Millipore Corporation) and transfering the filtrate to the test vessel. If dissolved oxygen was below 7.5 mg/l aeration was conducted to bring the dissolved oxygen concentration to above 7.5 mg/l. Test concentrations used in this study were 0 (control) and 100 mg/l. The test solution was renewed every 24 hours during the study. The appearance of the fresh and expired test media was checked at each renewal: there was no evidence of precipitation or of suspended or floating solids throughout the study.

Test organisms (species):

Oryzias latipes

Details on test organisms:

Test species: Medaka (Oryzias latipes)
Source: Tsunashima Fishing Co., Yokohama
Date of receipt: 08/02/2011
Total length: 2.36 cm (2.23-2.53 cm)
Body weight 0.096 g (0.074-0.121 g)
Age: Less than 1 year

Test type:

semi-static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Remarks on exposure duration:

None.

Hardness:

53 mg CaCO3/L

Test temperature:

23.1-23.7 oC

pH:

7.5-8.0

Dissolved oxygen:

6.6-7.8 mg/L

Salinity:

Not appropriate (freshwater test).

Nominal and measured concentrations:

Loading rates: 0 (control), 100 mg/L.
Mean measured concentrations of the test substance were 0.468 mg/L based on aluminium analysis and 0.360 mg/L based on titanium analysis.

Details on test conditions:

See methods below

Reference substance (positive control):

yes

Remarks:

The sensitivity of the fish was checked in a separate study performed on 2011-02-14 with the reference substance CuSO4(II)*5H2O.

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 0.36 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Remarks:

derived from measured titanium concentration

Basis for effect:

mortality (fish)

Details on results:

No mortality or abnormal symptoms were noted in the control or test solution.
The 96hr LC50 based on aluminium analysis was >0.468 mg dialuminium titanium pentaoxide/L
The 96hr LC50 based on titanium analysis was >0.360 mg dialuminium titanium pentaoxide/L

Results with reference substance (positive control):

The 96-h LC50 of CuSO4(II)*5H2O was 0.51 mg/L (95% CL: 0.34-0.76 mg/L), compared to a 16-yr laboratory mean of 0.8+/-0.27 mg/L (n = 61).

Reported statistics and error estimates:

As this is a limit test the LC50 of dialuminium titanium pentaoxide was determined in relation to the single applied concentration rather than by statistical methods.

Sublethal observations / clinical signs:

Table 1.         Concentrations of dialuminium titanium pentaoxide derived from measured concentrations of dissolved aluminium during a 96 hour acute toxicity test with O. latipes under semi-static conditions.  Loading rate, mg test substance/L Measured concentration (mg dialuminium titanium pentaoxide-equiv/L), based on dissolved aluminium analysis 0 h fresh 24 h expired 72 h fresh 96 h expired mean 0 (control) [0.0884] [0.0827] [0.0887] [0.0840] - 100 [0.537] [0.542] [0.564] [0.573] - 100, background-corrected 0.449 0.459 0.475 0.489 0.468 LOD:      Limit of detection (0.04 mg-equiv/L). [  ]          Measured concentration including background measured in control.     Table 2.         Concentrations of dialuminium titanium pentaoxide derived from measured concentrations of dissolved titanium during a 96 hour acute toxicity test with O. latipes under semi-static conditions.  Loading rate, mg test substance/L Measured concentration (mg dialuminium titanium pentaoxide-equiv/L), based on dissolved titanium analysis 0 h fresh 24 h expired 72 h fresh 96 h expired mean 0 (control) [< 0.04] [< 0.04] [< 0.04] [< 0.04] < LOD 100 [0.345] [0.360] [0.361] [0.376] - 100, background-corrected 0.345 0.360 0.361 0.376 0.360 LOD:      Limit of detection (0.04 mg-equiv/L).  [  ]          Measured concentration including background.      Table 3.         Effects on O. latipes following 96 hour exposure to dialuminium titanium pentaoxide under semi-static conditions Loading rate, mg test substance/L Mortality (%)^aand sub-lethal effects[%] 3 h 24 h 48 h 72 h 96 h 0 (control) 0 [0] 0 [0] 0 [0] 0 [0] 0 [0] 100^b 0 [0] 0 [0] 0 [0] 0 [0] 0 [0] a             Based on 10 fish per treatment.  b             Achieved exposure was 0.360 mg-equiv/L and 0.468 mg-equiv/L, based on mean measured concentrations of dissolved titanium and dissolved aluminium, respectively.

Validity criteria fulfilled:

yes

Conclusions:

The 96hr LC50 of dialuminium titanium pentaoxide to O. latipes was determined to be >0.360 mg/L, the maximum soluble concentration achieved under the test conditions at a loading rate of 100 mg/L, based on the mean measured concentration of dissolved titanium. The 96hr LC50 was >0.468 mg/L based on the mean measured (background-corrected) concentration of dissolved aluminium.

Executive summary:

The acute toxicity of dialuminium titanium pentaoxide to medaka (Oryzias latipes) was determined in a study carried out under semi-static conditions in accordance with OECD guideline 203 ''Fish, Acute Toxicity Test''. As the test substance is a poorly soluble inorganic compound, the test organisms were exposed to the test substance dissolved at the maximum saturation level, separated by filtration from a system loaded at a nominal rate of 100 mg/L. Concentrations of dissolved aluminium and titanium were determined by ICP-MS which were then used to calculate equivalent concentrations of the test substance. The test organisms were kept in 5 L aquaria and test solutions were renewed every 24 hours. After 96 hours of exposure no mortality or abnormal effects were observed. The 96hr LC50 of dialuminium titanium pentaoxide was determined to be >0.468 mg/L based on aluminium analysis and >0.360 mg/L based on titanium analysis. All validity criteria were met and this study is considered reliable without restriction.

Description of key information

A key study is provided for the assessment of short-term toxicity to fish. The study was conducted in accordance with GLP and OECD test guidelines with no deviations.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

0.36 mg/L

Additional information

The acute toxicity of dialuminium titanium pentaoxide to fish was determined in a study which was carried out in accordance with OECD guideline 203 ''Fish, Acute Toxicity Test''. As the test substance is a poorly soluble inorganic compound, medaka (Oryzias latipes) were exposed to test solution prepared at its maximum achievable saturation level. Concentrations of aluminium and titanium were determined by ICP-MS which were then used to calculate equivalent concentrations of the test substance. The loading rate used in the test was 100 mg/L. After 96 hours of exposure no mortality or abnormal effects were observed. The 96hr LC50 was determined to be >0.468 mg/L based on aluminium analysis and >0.360 mg/L based on titanium analysis. All validity criteria were met and this study is considered reliable without restriction.