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Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
other: read across from analogue substance
Adequacy of study:
weight of evidence
Study period:
June 17 - June 21 1991
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
ISO 7346-1 (Determination of the Acute Lethal Toxicity of Substances to a Freshwater Fish [Brachydanio rerio Hamilton-Buchanan (Teleostei, Cyprinidae)] - Part 2: Semi-static method)
Deviations:
not specified
GLP compliance:
yes
Analytical monitoring:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: The specimen is insoluble in water. The substance was weighed directly into the test vessel. Each test batch was mixed for 10 seconds with an Ultraturrax.
Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
TEST ORGANISM
- Common name: zebrafish
- Source: West Aquarium, delivered on 04 January 1991
- All other template details: Not reported

ACCLIMATION
- Type and amount of food: Altromin 1413, equivalent to A-1410 but roughly pelleted rather than milled
- Health during acclimation (any mortality observed): 0.1% mortality observed a week before test initiation
- No pretreatment of test animals
- All other template details: not reported

QUARANTINE (wild caught): not applicable
Test type:
semi-static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Post exposure observation period:
Not reported
Hardness:
Not reported (water was made per specifications in ISO 7346 protocol
Test temperature:
22.5 - 23.5 °C
pH:
7.7 - 8.1
Dissolved oxygen:
initially, 94-97 %
decreased to 24-78 % for the test containers by the end of the experiment
Nominal and measured concentrations:
Nominal: 0, 300, 1000, 3000 mg/l
Details on test conditions:
TEST SYSTEM: not reported

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: according to ISO 7346 II
- No other details reported

OTHER TEST CONDITIONS: not reported

EFFECT PARAMETERS MEASURED: mortality measured every 24 hours

TEST CONCENTRATIONS
- Spacing factor for test concentrations:
- Justification for using less concentrations than requested by guideline:
- Range finding study
- Test concentrations:
- Results used to determine the conditions for the definitive study:
Reference substance (positive control):
no
Duration:
96 h
Dose descriptor:
LC0
Effect conc.:
300 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Remarks on result:
other: 147 mg/l active ingredient
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
550 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Remarks on result:
other: 269 mg/l active ingredient
Duration:
96 h
Dose descriptor:
LC100
Effect conc.:
1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Remarks on result:
other: 490 mg/l active ingredient
Details on results:
See Table 1 for mortality data
Because of the insolubility of the substance, a quantitative assessment could not be made
Sublethal observations / clinical signs:

Table 1. Raw Mortality Data

 

Concentration (mg/l)

Test time (hr)

0

6

24

48

72

96

0

0

0

0

0

0

0

300

0

0

0

0

0

0

1000

0

0

60

100

100

100

30000

0

30

100

100

100

100

Conclusions:
A 96-hr test with Brachydanio rario according to ISO 7346 II with the test material resulted in an LC0 of 147 mg/l active ingredient, an LC50 of 269 mg/l active ingredient, and an LC100 of 490 mg/ L active ingredient. Overall, the test material has a low toxicity to fish.
Executive summary:

The low test-sulfo-rapeseed oil, according to the UBA method proposal of 01.06.1982 "lethal effect in zebrafish Brachidanio rerio" (LC 0, LC 50, LC 100, 48 - 96 hours) ISO 7346 ll, had an LC0 = 147 mg/ L active ingredient and has a low toxicity to fish.

Description of key information

LC50, 96h = 5.4 mg/l (general assessment)

Key value for chemical safety assessment

Fresh water fish

Fresh water fish
Effect concentration:
5.4 mg/L

Additional information

Refer to the assessment in the general summary

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