Castor oil, sulfated, ammonium salt

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

other: read across from analogue substance

Adequacy of study:

weight of evidence

Study period:

June 17 - June 21 1991

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

ISO 7346-1 (Determination of the Acute Lethal Toxicity of Substances to a Freshwater Fish [Brachydanio rerio Hamilton-Buchanan (Teleostei, Cyprinidae)] - Part 2: Semi-static method)

Deviations:

not specified

GLP compliance:

yes

Analytical monitoring:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: The specimen is insoluble in water. The substance was weighed directly into the test vessel. Each test batch was mixed for 10 seconds with an Ultraturrax.

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: zebrafish
- Source: West Aquarium, delivered on 04 January 1991
- All other template details: Not reported

ACCLIMATION
- Type and amount of food: Altromin 1413, equivalent to A-1410 but roughly pelleted rather than milled
- Health during acclimation (any mortality observed): 0.1% mortality observed a week before test initiation
- No pretreatment of test animals
- All other template details: not reported

QUARANTINE (wild caught): not applicable

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Post exposure observation period:

Not reported

Hardness:

Not reported (water was made per specifications in ISO 7346 protocol

Test temperature:

22.5 - 23.5 °C

pH:

7.7 - 8.1

Dissolved oxygen:

initially, 94-97 %
decreased to 24-78 % for the test containers by the end of the experiment

Nominal and measured concentrations:

Nominal: 0, 300, 1000, 3000 mg/l

Details on test conditions:

TEST SYSTEM: not reported

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: according to ISO 7346 II
- No other details reported

OTHER TEST CONDITIONS: not reported

EFFECT PARAMETERS MEASURED: mortality measured every 24 hours

TEST CONCENTRATIONS
- Spacing factor for test concentrations:
- Justification for using less concentrations than requested by guideline:
- Range finding study
- Test concentrations:
- Results used to determine the conditions for the definitive study:

Reference substance (positive control):

no

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

300 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: 147 mg/l active ingredient

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

550 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: 269 mg/l active ingredient

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: 490 mg/l active ingredient

Details on results:

See Table 1 for mortality data
Because of the insolubility of the substance, a quantitative assessment could not be made

Sublethal observations / clinical signs:

Table 1. Raw Mortality Data

 

Concentration (mg/l)	Test time (hr)
	0	6	24	48	72	96
0	0	0	0	0	0	0
300	0	0	0	0	0	0
1000	0	0	60	100	100	100
30000	0	30	100	100	100	100

Conclusions:

A 96-hr test with Brachydanio rario according to ISO 7346 II with the test material resulted in an LC0 of 147 mg/l active ingredient, an LC50 of 269 mg/l active ingredient, and an LC100 of 490 mg/ L active ingredient. Overall, the test material has a low toxicity to fish.

Executive summary:

The low test-sulfo-rapeseed oil, according to the UBA method proposal of 01.06.1982 "lethal effect in zebrafish Brachidanio rerio" (LC 0, LC 50, LC 100, 48 - 96 hours) ISO 7346 ll, had an LC0 = 147 mg/ L active ingredient and has a low toxicity to fish.

Description of key information

LC50, 96h = 5.4 mg/l (general assessment)

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

5.4 mg/L

Additional information

Refer to the assessment in the general summary