Registration Dossier
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EC number: 269-124-2 | CAS number: 68187-77-9
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- other: read across from analogue substance
- Adequacy of study:
- weight of evidence
- Study period:
- June 17 - June 21 1991
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- ISO 7346-1 (Determination of the Acute Lethal Toxicity of Substances to a Freshwater Fish [Brachydanio rerio Hamilton-Buchanan (Teleostei, Cyprinidae)] - Part 2: Semi-static method)
- Deviations:
- not specified
- GLP compliance:
- yes
- Analytical monitoring:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: The specimen is insoluble in water. The substance was weighed directly into the test vessel. Each test batch was mixed for 10 seconds with an Ultraturrax. - Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Common name: zebrafish
- Source: West Aquarium, delivered on 04 January 1991
- All other template details: Not reported
ACCLIMATION
- Type and amount of food: Altromin 1413, equivalent to A-1410 but roughly pelleted rather than milled
- Health during acclimation (any mortality observed): 0.1% mortality observed a week before test initiation
- No pretreatment of test animals
- All other template details: not reported
QUARANTINE (wild caught): not applicable - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Post exposure observation period:
- Not reported
- Hardness:
- Not reported (water was made per specifications in ISO 7346 protocol
- Test temperature:
- 22.5 - 23.5 °C
- pH:
- 7.7 - 8.1
- Dissolved oxygen:
- initially, 94-97 %
decreased to 24-78 % for the test containers by the end of the experiment - Nominal and measured concentrations:
- Nominal: 0, 300, 1000, 3000 mg/l
- Details on test conditions:
- TEST SYSTEM: not reported
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: according to ISO 7346 II
- No other details reported
OTHER TEST CONDITIONS: not reported
EFFECT PARAMETERS MEASURED: mortality measured every 24 hours
TEST CONCENTRATIONS
- Spacing factor for test concentrations:
- Justification for using less concentrations than requested by guideline:
- Range finding study
- Test concentrations:
- Results used to determine the conditions for the definitive study: - Reference substance (positive control):
- no
- Duration:
- 96 h
- Dose descriptor:
- LC0
- Effect conc.:
- 300 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: 147 mg/l active ingredient
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 550 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: 269 mg/l active ingredient
- Duration:
- 96 h
- Dose descriptor:
- LC100
- Effect conc.:
- 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: 490 mg/l active ingredient
- Details on results:
- See Table 1 for mortality data
Because of the insolubility of the substance, a quantitative assessment could not be made - Sublethal observations / clinical signs:
Table 1. Raw Mortality Data
Concentration (mg/l)
Test time (hr)
0
6
24
48
72
96
0
0
0
0
0
0
0
300
0
0
0
0
0
0
1000
0
0
60
100
100
100
30000
0
30
100
100
100
100
- Conclusions:
- A 96-hr test with Brachydanio rario according to ISO 7346 II with the test material resulted in an LC0 of 147 mg/l active ingredient, an LC50 of 269 mg/l active ingredient, and an LC100 of 490 mg/ L active ingredient. Overall, the test material has a low toxicity to fish.
- Executive summary:
The low test-sulfo-rapeseed oil, according to the UBA method proposal of 01.06.1982 "lethal effect in zebrafish Brachidanio rerio" (LC 0, LC 50, LC 100, 48 - 96 hours) ISO 7346 ll, had an LC0 = 147 mg/ L active ingredient and has a low toxicity to fish.
Reference
Description of key information
LC50, 96h = 5.4 mg/l (general assessment)
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 5.4 mg/L
Additional information
Refer to the assessment in the general summary
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