Registration Dossier

Data platform availability banner - registered substances factsheets

Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Short-term toxicity to fish

Currently viewing:

Administrative data

Link to relevant study record(s)

Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study
Justification for type of information:
Experimental test result performed according to the guideline.
Qualifier:
according to guideline
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Principles of method if other than guideline:
Study was conducted to access the effect of test chemical 1-phenylethanol (CAS No. 98-85-1) on the growth of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test).
GLP compliance:
not specified
Specific details on test material used for the study:
IUPAC name: 1-phenylethan-1-ol
Mol. formula: C8H10O
Molecular Weight: 122.166 g/mol
Substance form: Colourless liquid
Substance type: organic
InChI: 1S/C8H10O/c1-7(9)8-5-3-2-4-6-8/h2-7,9H,1H3
Smiles :c1(ccccc1)C(C)O

Analytical monitoring:
not specified
Vehicle:
not specified
Details on test solutions:
The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 1ml of the test substance in 10 liters of potable water (passed through reverse osmosis system) with continuous stirring for achieving test concentrations of 100 mg/L, respectively.
Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
TEST ORGANISM
- Common name:Zebra Fish
- Strain:Danio rerio
- Source: Ashish Fish Center, Dharampeth, Nagpur, MS (India)
- Length at study initiation (length definition, mean, range and SD): average 2.28 cm
- Weight at study initiation (mean and range, SD): average 0.116 g
-Housing:The fishes were kept in a static tank in tap water passed through reverse osmosis system, under natural conditions along with proper feed and aeration.
-Duration of housing: 15 days
-Photoperiod: 12 hours light and 12 hours darkness
-Diet : Standard brand feed, once daily
-Acclimatization: seven days
-feeding frequency: Once in a day
-Feeding : Once in a day up to sixth day of acclimatization
Test type:
static
Water media type:
freshwater
Total exposure duration:
96 h
Hardness:
42.5 mg of CaCO3
Test temperature:
28 °C
pH:
7.0
Dissolved oxygen:
7.1 mg/l
Nominal and measured concentrations:
100 mg/l
Details on test conditions:
TEST SYSTEM
- Test vessel: Plastic aquaria
- Material, size, headspace, fill volume: 5 liters of plastic aquaria filled with 2 liter of water
- Aeration: Aeration in test vessels was provided 1 day before the start of experiment
- No. of organisms per vessel: 8 fishes

OTHER TEST CONDITIONS
- Photoperiod: 12 hours light and 12-hour darkness

Reference substance (positive control):
not specified
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Sublethal observations / clinical signs:

8.1. Mortality and symptoms

Nominal conc. (mg/l)

Number of fish

Mortality (cumulated) after

24h

48h

72h

96h

0 (control)

8

-

-

-

-

100

8

-

-

-

-

Visible Symptoms: No visible symptoms were observed

Nominal conc. (mg/l)

Number of fish

Symptoms after

24h

48h

72h

96h

0 (control)

8

NS8

NS8

NS8

NS8

100

8

NS8

NS8

NS8

NS8

Explanation of symptoms:

A: apathy

N: narcotic-like state

C: swimming near water surface

D: swimming at the bottom of bowl

M: Slow movements compared to control

NS: No abnormal symptoms

Number behind symbol for symptom= number of affected fish

8.2. pH and oxygen content:

Nominal conc. (mg/l)

pH readings after

24h

48h

72h

96h

0 (control)

7.6

6.0

6.9

6.7

100

6.5

5.8

5.9

5.8

Nominal conc. (mg/l)

Oxygen content (mg/l) after

24h

48h

72h

96h

0 (control)

7.1

6.5

6.5

6.5

100

6.7

6.2

6.3

6.2

8.3. Test temperature

Nominal conc. (mg/l)

Temperature (°C) after

24h

48h

72h

96h

0 (control)

28

28

28

28

100

28

28

28

28

 

Validity criteria fulfilled:
yes
Conclusions:
Based on nominal concentrations, experimental median lethal Concentrations [LC-50 (96 h)] for 1-phenylethan-1-ol CAS No.98-85-1 on Zebra Fish Danio rerio was determine to be >100 mg/l.
Executive summary:

Study was conducted to access the effect of test chemical 1-phenylethan-1-ol on the growth of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test). The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 1ml of the test substance in 10 liters of potable water (passed through reverse osmosis system) with continuous stirring for achieving test concentrations of 100 mg/L, respectively and Zebra FishDanio reriowere exposed to these concentration for 96 hours.Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. After 96 hours of exposure to test item 1-phenylethan-1-ol to various nominal test concentrations, LC50 was determine to be >100 mg/l . Based on the LC50, it can be consider that the chemical was 1-phenylethan-1-ol and can be consider to be not classified as per the CLP classification criteria.

Description of key information

Short term toxicity to fish:

Study was conducted to access the effect of test chemical1-phenylethan-1-olon the growth of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test).The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 1ml of the test substance in 10 liters of potable water (passed through reverse osmosis system) with continuous stirring for achieving test concentrations of 100 mg/L, respectivelyand Zebra FishDanio reriowere exposed to these concentration for 96 hours.Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. After 96 hours of exposure to test item1-phenylethan-1-olto various nominal test concentrations, LC50 was determine to be >100 mg/l . Based on the LC50, it can be consider that the chemical was1-phenylethan-1-oland can be consider to be not classified as per the CLP classification criteria.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish
Effect concentration:
100 mg/L

Additional information

Short term toxicity to fish:

Data from experimental study as well as from peer reviewed journals were used to evaluate the short term toxicity to fish for the test chemical 1-phenylethan -1-olon.

Study was conducted to access the effect of test chemical1-phenylethan-1-olon the growth of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test).The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 1ml of the test substance in 10 liters of potable water (passed through reverse osmosis system) with continuous stirring for achieving test concentrations of 100 mg/L, respectivelyand Zebra FishDanio reriowere exposed to these concentration for 96 hours.Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. After 96 hours of exposure to test item1-phenylethan-1-olto various nominal test concentrations, LC50 was determine to be >100 mg/l . Based on the LC50, it can be consider that the chemical was1-phenylethan-1-ol and can be consider to be not classified as per the CLP classification criteria.

The above experimental study was supported by experimental study summarized in peer reviewed journal for the test chemical.

Short term toxicity of test study was conducted according to the OECD 203 method under static conditions for the test material 1-phenylethanol.The median lethal concentration of test material 1-phenylethanol on Zebra fish after 96 h was observed to be 100 mg/l.

Based on the above effect concentartion it can be considered that test material 1-phenylethan-1-ol was not toxic to fish and can be considered as not classified as per CLP criteria.