[No public or meaningful name is available]

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

13 - 15 Oct 1998

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Guideline study with acceptable restrictions: A very high detection limit (10 mg/L) disabled an analytical monitoring of the test substance.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1998

Report date:

1998

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Fatty acids, C16-18 and C18-unsatd., tetraesters with pentaerythritol

EC Number:

271-694-2

EC Name:

Fatty acids, C16-18 and C18-unsatd., tetraesters with pentaerythritol

Cas Number:

68604-44-4

IUPAC Name:

68604-44-4

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

- Concentrations: 100 mg/L

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: The test substance was weighed directly to the test medium and stirred for one day. Oily drops were recorded on the surface of the test solution after 2 h. The test solution was filtered through a glass fibre filter.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Source: Ephippia were obtained from BioInternational B.V., NJ Horn Netherlands (1998/09/03, batch 980803)
- Method of breeding: The Ephippia were incubated in M4-medium (Elendt) at approx. 20 °C and approx. 4800 lux (constant illumination). The neonates were incubated in M4-medium (Elendt) at 20 °C and approx. 900 lux (16 h light/8 h dark). They were fed with green algae Scenedesmus subspicatus (last feeding approx. 5 h before study initiation).

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Test conditions

Test temperature:

20.0-20.2 °C

pH:

8.0 (48 h)

Dissolved oxygen:

93-94% saturation

Nominal and measured concentrations:

Nominal: 100 mg/L
Measured: < 10 mg/L (detection limit)

Details on test conditions:

TEST SYSTEM
- Test vessel: beaker
- Type: closed, slightly covered with watchglasses
- Material: glas, size: 100 mL, fill volume: 50 mL
- No. of organisms per vessel: 10
- No. of vessels per concentration: 2
- No. of vessels per control: 2

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: M4-medium according to Elendt

OTHER TEST CONDITIONS
- Photoperiod: 16 h light/8 h dark
- Light intensity: approx. 900 lux

EFFECT PARAMETERS MEASURED: Immobilisation was recorded after 24 and 48 h test duration.

Reference substance (positive control):

yes

Remarks:

sodium dichromate

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

EL0

Effect conc.:

>= 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Remarks:

(WAF)

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EL50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Remarks:

(WAF)

Basis for effect:

mobility

Effect concentrations 3

Duration:

48 h

Dose descriptor:

EL100

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Remarks:

(WAF)

Basis for effect:

mobility

Details on results:

- Behavioural abnormalities: No behavioural abnormalities were observed.
- Mortality of control: 0%

Results with reference substance (positive control):

- Results with reference substance valid? yes
- EC50: 1.4 mg/L (24 h)

Any other information on results incl. tables

No mortality was observed in any treatment and the control.