1,2-dichloro-4-(trichloromethyl)benzene

Endpoint summary

Description of key information

The LD50 in male rat is 1424 mg/kg bw.

The LD50 in female rat is 1600 mg/kg bw.

Key value for chemical safety assessment

Acute toxicity: via oral route

Endpoint conclusion

Endpoint conclusion:

adverse effect observed

Dose descriptor:

LD50

Value:

1 424 mg/kg bw

Acute toxicity: via inhalation route

Endpoint conclusion

Endpoint conclusion:

no study available

Acute toxicity: via dermal route

Endpoint conclusion

Endpoint conclusion:

no study available

Additional information

A toxicity study in which Wistar rats (5 males and 5 females/dose) received a single oral administration of the test article 3,4 -Dichlorbenzotrichloride at the doses of 1000, 1600 and 2000 mg/kg bw was conducted.

The test method was in accordance with OECD Guideline n. 401. The test article was administered in a Chromophor solution (2% v/v) in deionised water at the volume of 10 mL/kg bw .

The animals were weighed before treatment and on days 4, 8 and 14. They were clinically observed for 14 days after treatment.Animals which died and animals killed at the end of the study were submitted to a thorough autopsy. One male treated at 1000 mg/kg bw died on day 7. No female treated at 1000 mg/kg bw died on day 7. Three males treated at 1600 mg /kg bw died within days 2 and 5. At the same dose, 2 females died within days 3 and 10. Four males treated at 2000 mg/ kg bw died within day 1 and 6. At the same dose, 5 females died within day 6 and 11 of treatment.

Treated animals showed the following symptoms: lung dilatation, pale or coloured liver, pale spleen, pale or grey kidney with deformed structure, reddish pelvis, reddish stomach walls, thickened or thinned and with ulceration, bladder containing urine from clear to reddish, hard fecesl in the colon, small intestine with dark mucus. Recovery of all surviving rats was achieved from 15 minutes to 12 days. On days 4 and 8, significant body weight loss in some of the animals was registered, then recovered after the first or the second week of application.

Justification for classification or non-classification

According to the CLP Regulation (EC 1272/2008), 3.1 Acute toxicity section, substances can be allocated to one of four toxicity categories based on acute toxicity by oral, dermal or inhalation route according to the numeric criteria. Acute toxicity values are expressed as (approximate) LD50 (oral, dermal) or LC50 (inhalation) values or as acute toxicity estimates (ATE).

The oral LD50 value was established to be 1424 mg/kg body weight, therefore test substance is classified for acute oral toxicity Category 4 (oral acute toxicity category 4: 300 < ATE ≤ 2000 mg/kg bw), according to the CLP Regulation.