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Diss Factsheets

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2003
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2003
Report date:
2003

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
GLP compliance:
yes

Test material

Constituent 1
Test material form:
solid: particulate/powder
Details on test material:
Batch number: 022401

Sampling and analysis

Analytical monitoring:
yes
Remarks:
The actual concentrations of the test substance in the test media were determined by HPLC before the test and after 48 hours.

Test solutions

Vehicle:
no
Remarks:
Immediately before the beginning of the test, a 100 mg/l solution was prepared by dissolving 25.0 mg test substance in 250 ml reconstituted water. The lower concentrations were then obtained by diluting with reconstituted water.
Details on test solutions:
Identity and concentration of auxiliary solvent for dispersal: The test substance was dissolved in dilution water.

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
Neonates of Daphnia magna, less than 24 hours old, were obtained by hatching ephippia, supplied in the microbiotest "Daphtoxkit F TM "(Daphnia rnagna; MicroBioTests Inc., lndustriezone "De Prijkels", Venecoweg 19 , 9810 Nazamth, Belgium). Batch No. of the ephippia: M291002. The neonates were collected for the test at 86 hours after initiation of hatching.

Study design

Test type:
not specified
Water media type:
other: Immediately before the beginning of the test a 100 mg/l solution was prepared by dissolving 25.0 mg test substance in 250 ml reconstituted water. The lower concentrations were then obtained by diluting with reconstituted water.
Total exposure duration:
48 h

Test conditions

Hardness:
250 mg CaCO3/L
Test temperature:
Temperature: approximately 21 °C throughout the test.
pH:
pH: between 7.5 and 8.5 in the test substance solutions.
Dissolved oxygen:
Dissolved oxygen concentration: at least 8.3 mg/L.
Nominal and measured concentrations:
Nominal concentrations (mg/L): 0, 10, 18, 32, 56, 100.
At the end of the test the measured concentrations of "STl571 F8" were between 96 and 101 % of the actual starting concentration.

Results and discussion

Effect concentrationsopen allclose all
Key result
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
60 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
mobility
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
37 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
mobility
Key result
Duration:
48 h
Dose descriptor:
EC100
Effect conc.:
100 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
mobility
Key result
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
10 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
At the end of the test the measured concentrations of "STl571 F8" were between 96 and 101 % of the actual starting concentration.
Immobilisation of control group daphnids was O % at the end of the test (guideline: maximum of 10 %).
As some daphnids in the test substance groups wem trapped at the water surface there is a possible influence of the test substance to the water surface tension.
There were no control daphnids trapped at the water surface.
Temperature was stable during the test period.
Dissolved oxygen concentration in the test vessels was not lower than 8.4 mg/L (EC guideline: higher than 3 mg/L).

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Remarks:
Dissolved oxygen concentration in the test vessels was not lower than 8.4 mg/L (EC guideline: higher than 3 mg/L).
Conclusions:
Acute aquatic toxicity: Cat.3