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EC number: 413-910-1 | CAS number: 1467668-33-2 LUPEROX 610-E-35; LUPEROX 610-E-50; LUPEROX 610-EN-50; LUPEROX 610-M-50; LUPERSOL 610; LUPERSOL 610-M-50
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1992
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 992
- Report date:
- 1992
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- 3-hydroxy-1,1-dimethylbutyl 2-ethyl-2-methylheptaneperoxoate
- EC Number:
- 413-910-1
- EC Name:
- 3-hydroxy-1,1-dimethylbutyl 2-ethyl-2-methylheptaneperoxoate
- Cas Number:
- 1467668-33-2
- Molecular formula:
- C16H32O4
- IUPAC Name:
- 4-hydroxy-2-methylpentan-2-yl 2-ethyl-2-methylheptaneperoxoate
- Details on test material:
- - Batch number: sample no.1
- Purity: 90.5 %
- Before the treatment, the test substance was stored at -20°C and protected from light and was stored at +4 °C during the treatment
- The pH of the test substance specified in the test article description was between 5 and 7.
Constituent 1
Method
Species / strain
- Species / strain / cell type:
- other: TA 1535, TA 1537, TA 102, TA 98 , TA 100 and TA 102
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9 fraction coming from liver of male Sprague-Dawley rats induced with Aroclor 1254 (500 mg/kg) by the intraperitoneal route
- Test concentrations with justification for top dose:
- 12.5, 25, 31.25, 50, 62.5, 100, 125, 200, 250, 500, 750 and 1000 µg/plate.
- Vehicle / solvent:
- DMSO
Controls
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: With S9: sodium azide, 9-amino-acridine, 2-nitrofluorene, mitomycine C / Without S9: 2-anthramine, Danthron
- Details on test system and experimental conditions:
- After a preliminary assay to define the concentrations to be used for the mutagenicity study, the test substance was tested on two independent assays.
Each assay was carried out both in the absence and in the presence of a metabolic activation system, the S9 mix, prepared from a liver microsomal fraction S9 of rats treated with Aroclor 1254.
The methods used were:
- the direct plate incorporation method for the 2 assays without S9 mix and for the first assay with S9 mix,
- the preincubation method (1 h, 37°C) for the second assay with S9 mix
The concentrations were:
- without S9 mix: 125, 250, 500, 750 and 1000 µg/plate for the first test, 31.25, 62.5, 125, 250 and 500 µg/plate for the first repeat test, 12.5, 25, 50, 100 and 200 µg/plate for the second test.
- with S9 mix: 125, 250, 500, 750 and 1000 µg/plate for both tests and for the TA 1537 and TA 98 strains in the third test.
The negative and solvent control results were equivalent to those usually obtained in our laboratory. The number of revertants induced by the positive controls was higher than the spontaneous one, which demonstrated the sensitivity of this test and the efficacy of the S9 mix throughout this study.
Results and discussion
Test results
- Species / strain:
- other: S. typhimurium TA 1535, TA 1537, TA 98, TA 100 and TA102
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity, but tested up to precipitating concentrations
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Additional information on results:
- The test substance did not induce any significant increase in the revertant number with or without S9 mix in any of the 5 strains.
- Remarks on result:
- other: other: TA 1535, TA 1537, TA 102, TA 98 and TA 100.
- Remarks:
- Migrated from field 'Test system'.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative
Under the experimental conditions, the substance did not show mutagenic activity in the Ames test. - Executive summary:
The in vitro potential mutagenic activity of the substance was investigated by the Ames test using 5 strains of bacteria Salmonella typhimurium: TA 1535, TA 1537, TA 102, TA 98 and TA 100 (OECD 471, GLP).
After a preliminary assay to define the concentrations to be used for the mutagenicity study, the test substance was tested on two independent assays. Each assay was carried out both in the absence and in the presence of a metabolic activation system, the S9 mix, prepared from a liver microsomal fraction S9 of rats treated with Aroclor 1254. The methods used were:
- the direct plate incorporation method for the 2 assays without S9 mix and for the first assay with S9 mix,
- the preincubation method (1 h, 37'C) for the second assay with S9 mix.
The concentrations were:
- without S9 mix:
. 125, 250, 500, 750 and 1000 gg/plate for the first test,
. 31.25, 62.5, 125, 250 and 500 µg/plate for the first repeat test,
. 12.5, 25, 50, 100 and 200 µg/plate for the second test.
- with S9 mix:
. 125, 250, 500, 750 and 1000 µg/plate for both tests and for the TA 1537 and TA 98 strains in the third test.
The negative and solvent control results were equivalent to those usually obtained in our Laboratory. The number of revertants induced by the positive controls was higher than the spontaneous one, which demonstrated the sensitivity of this test and the efficacy of the S9 mix throughout this study.
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