Registration Dossier
Registration Dossier
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EC number: 247-384-8 | CAS number: 25973-55-1
Specific investigations: other studies
Administrative data
- Endpoint:
- endocrine system modulation
- Type of information:
- experimental study
- Adequacy of study:
- other information
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Reliable study report (not GLP)
Data source
Reference
- Reference Type:
- publication
- Title:
- Unnamed
- Year:
- 2 003
Materials and methods
- Principles of method if other than guideline:
- 11 kinds of UV stabilizers used in food contact plastics were tested for their estrogenic activity by the yeast two-hybrid assay.
- GLP compliance:
- no
- Type of method:
- in vitro
- Endpoint addressed:
- other: estrogenic activity
Test material
- Reference substance name:
- 2-(2H-benzotriazol-2-yl)-4,6-ditertpentylphenol
- EC Number:
- 247-384-8
- EC Name:
- 2-(2H-benzotriazol-2-yl)-4,6-ditertpentylphenol
- Cas Number:
- 25973-55-1
- Molecular formula:
- C22H29N3O
- IUPAC Name:
- 2-(2H-benzotriazol-2-yl)-4,6-bis(1,1-dimethylpropyl)phenol
Constituent 1
Test animals
- Species:
- other: not applicable, in vitro assay
- Strain:
- other: not applicable
Administration / exposure
- Route of administration:
- other: not applicable
- Vehicle:
- other: DMSO
- Details on exposure:
- - The yeast cells were preincubated overnight at 30°C with vigorous shaking in a synthetic dropout (SD) medium free from tryptophan and leucine.
- test chemical solution was added and the mixture was incubated for 4 h at 30°C. - Duration of treatment / exposure:
- 4 h
Doses / concentrations
- Remarks:
- Doses / Concentrations:
1 mM - 0.1 µM
Basis:
nominal conc.
Examinations
- Examinations:
- The results were evaluated based on relative activity, expressed as 10% relative effective concentration (REC 10), which is the concentration of the
test chemical showing 10% of the agonist activity of 1 E-6 M 17ß-Estradiol, the highest activity level of 17ß-Estradiol. - Positive control:
- 17ß-Estradiol
Results and discussion
- Details on results:
- REC 10 < 1.0 E -4
None of the tested Benzotriazole compounds - among these the test substance - showed estrogenic activity.
Applicant's summary and conclusion
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