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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
acute toxicity: dermal
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP test performed according to OECD guideline.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2013

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 402 (Acute Dermal Toxicity)
GLP compliance:
yes (incl. QA statement)
Test type:
standard acute method
Limit test:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
1,2-dihydro-5-nitro-3H-1,2,4-triazol-3-one
EC Number:
213-254-4
EC Name:
1,2-dihydro-5-nitro-3H-1,2,4-triazol-3-one
Cas Number:
932-64-9
Molecular formula:
C2H2N4O3
IUPAC Name:
1,2-dihydro-5-nitro-3H-1,2,4-triazol-3-one
Test material form:
solid: crystalline
Details on test material:
- Name of test material : 3-NITRO-1 ,2,4-TRIAZOL-5-0NE (NTO)
- Substance type: energetic explosive
- Physical state: light green to white crystalline solid with no odor
- Purity ca.99%
Specific details on test material used for the study:
Lot number: DDP12K0091-0002
Expiry: 31 December 2022
Purity: 100%

Test animals

Species:
rat
Strain:
Sprague-Dawley
Sex:
male/female
Details on test animals or test system and environmental conditions:
TEST ANIMALS
- Source: well known breeder
- Age at study initiation: 8 - 12 weeks
- Weight at study initiation:
- Fasting period before study: Not fasted
- Housing: individually housed in cages made of polypropylene or polycarbonate with a stainless steel mesh lid with wood based bedding.
- Diet (e.g. ad libitum): Standard laboratory rodent diet (RM1(E) SQC expanded pellet
- Water (e.g. ad libitum): Water was available ad libitum throughout the study
- Acclimation period: minimum of 5 days

ENVIRONMENTAL CONDITIONS
- Temperature (°C):
- Humidity (%):

- Photoperiod (hrs dark / hrs light): 12 h light/12 h of dark

IN-LIFE DATES: From: To:

Administration / exposure

Type of coverage:
occlusive
Vehicle:
other: methylcellulose
Details on dermal exposure:
TEST SITE
- Area of exposure:5 cm x 5 cm of skin
- % coverage: 10% of the total body surface area
- Type of wrap if used:porous gauze held in place with a non-irritating dressing and further covered by a waterproof dressing, encircled firmly around the trunk

REMOVAL OF TEST SUBSTANCE
- Washing (if done): yes
- Time after start of exposure: 24 h

TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 1.5 mL/kg
- Concentration (if solution): 1340 mg/mL in 0.5% methylcellulose
- Constant concentration used: yes
- For solids, paste formed: yes

Duration of exposure:
24 h
Doses:
2000 mg/kg body weight
No. of animals per sex per dose:
5
Control animals:
not required
Details on study design:
- Duration of observation period following administration: 14 days
- Frequency of observations and weighing:Day 1 (prior to dosing), Day 8 and Day 15
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight macroscopic pathology, dermal responses

Results and discussion

Effect levels
Sex:
male/female
Dose descriptor:
LD50
Effect level:
> 2 000 mg/kg bw
Based on:
test mat.
Mortality:
No
Clinical signs:
other: No treatment-related sign of toxicity. Local signs comprised test substance staining and isolated cases of transient bandage reaction
Gross pathology:
No findings
Other findings:
No

Applicant's summary and conclusion

Interpretation of results:
practically nontoxic
Remarks:
Migrated information Criteria used for interpretation of results: EU
Conclusions:
The dermal LD50 value for NTO to Sprague-Dawley rat was greater than 2 g/kg.