Bis(piperidinothiocarbonyl) hexasulphide

Bioaccumulation: aquatic / sediment

Link to relevant study record(s)

Reference

Endpoint:

bioaccumulation in aquatic species: fish

Type of information:

experimental study

Adequacy of study:

weight of evidence

Study period:

1982-1983

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 305 (Bioaccumulation in Fish: Aqueous and Dietary Exposure) -I: Aqueous Exposure Bioconcentration Fish Test

Deviations:

no

Principles of method if other than guideline:

QSAR calculation

GLP compliance:

no

Radiolabelling:

no

Details on sampling:

Sampled fish were measured and weighted. Then slices were prepared and mixed with 50 mL concentrated hydrochloric acid, 50 mL ethanol and 5 g stannous tin. Mixture was heated at 100°C for 90 minutes and subsequently cooled. Volume was completed to 200 mL with deionized water. A 40 mL sub-sample was taken and diluted with 50 mL deionized water. This sample was mixed with 10 g NaCl and 10 g potassium peroxyde. The sample was filtered and the organic matter was discarded. Sample pH was adjusted to 10-11. 0.5 mL of 4% 2,4 dinitrofluorobenzene in ethanol was added. After shaking, 20 mL of deionized water and 10 g sodium chloride. The sample was then extracted 4 times with 30 mL chloroform. Sample was then dried and diluted in acetonitrile for analysis.
The recovery rate by the above operation was 86.3%.

Water samples were mixed with sodium chloride and extracted with 30 mL chloroform. Samples were dried and rediluted in 10 mL chloroform for analysis. The recovery rate by the above operation was 91-95%.

Vehicle:

yes

Remarks:

Hydrogenated castor oil + olive oil + rock sugar

Details on preparation of test solutions, spiked fish food or sediment:

10g of rock sugar was added to 1g of the tested substance, fully mixed and pulverized in a grinding machine, HCO-20 20g and olive oil 10g were then added and mixed. Deionized water was added to this and fixed to a total volume of 1L, preparing a 1000ppm (w/v) dispersion. For convenience, all concentration displays are shown treating the purity of the tested substance to be 100%, and no purity corrections were performed.

Test organisms (species):

Cyprinus carpio

Details on test organisms:

- Species: Cyprinus carpio
- Mean weight: 25.6 g
- Mean length: 9.8 cm
- Mean fat content: 4.9% (Bligh and Dyer, 1959)

Route of exposure:

aqueous

Justification for method:

aqueous exposure method used for following reason: Study from 1982.

Test type:

flow-through

Water / sediment media type:

natural water: freshwater

Test temperature:

25°C

Salinity:

Freshwater

Details on test conditions:

TEST SYSTEM
- Test vessel: tank
- Material: glass
- Volume: 100 L
- Method of exposure: flow-through
- Flow rate: 582 L/day


- Acclimation period: 14 days at 25°C (test temperature)

Nominal and measured concentrations:

Nominal: 500 µg/L and 50 µg/L
Measured for the 500 µg/L group: 503 µg/L (2 weeks) ; 500 µg/L (4 weeks) ; 496 µg/L (6 weeks) ; 480 µg/L (8 weeks)
Measured for the 50 µg/L group: 48.8 µg/L (2 weeks) ; 51.0 µg/L (4 weeks) ; 51.0 µg/L (6 weeks) ; 50.6 µg/L (8 weeks)

Reference substance (positive control):

no

Lipid content:

4.8 %

Time point:

start of exposure

Key result

Type:

BCF

Value:

<= 32 L/kg

Basis:

not specified

Validity criteria fulfilled:

yes

Conclusions:

The BCF of the test substance was lower than 32 L/Kg whole body weight.

Executive summary:

Bioconcentration of dipentamethylenethiuram tetrasulfide was tested on Cyprinus carpio according a protocol similar to OECD 305 TG. The test involved two test concentrations (500 and 50 µg/L) under flow-through conditions. Test duration was 8 weeks. In both groups, BCFs were lower than 32 L/kg. This means that the degradation products of dipentamethylenethiuram hexasulfide with strictly less than 5 sulfides do not have a high potential for bioaccumulation.

Key value for chemical safety assessment

BCF (aquatic species):

32 L/kg ww

Additional information

The bioconcentration factor (BCF) of dipentamethylene thiuram hexasulphide is unknown. However, the BCF of dipentamethylenethiuram tetrasulfide was shown to be lower than 32 L/kg in a bioconcentration in fish study.

Therefore, it can be conclude that the smallest degradation products of DPTH are not B/vB and thus, not PBT/vPvB.

Additional information are available in the position paper attached to the IUCLID dossier.