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Diss Factsheets

Environmental fate & pathways

Bioaccumulation: aquatic / sediment

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Link to relevant study record(s)

bioaccumulation in aquatic species: fish
Type of information:
experimental study
Adequacy of study:
weight of evidence
Study period:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
equivalent or similar to guideline
OECD Guideline 305 (Bioaccumulation in Fish: Aqueous and Dietary Exposure) -I: Aqueous Exposure Bioconcentration Fish Test
Principles of method if other than guideline:
QSAR calculation
GLP compliance:
Details on sampling:
Sampled fish were measured and weighted. Then slices were prepared and mixed with 50 mL concentrated hydrochloric acid, 50 mL ethanol and 5 g stannous tin. Mixture was heated at 100°C for 90 minutes and subsequently cooled. Volume was completed to 200 mL with deionized water. A 40 mL sub-sample was taken and diluted with 50 mL deionized water. This sample was mixed with 10 g NaCl and 10 g potassium peroxyde. The sample was filtered and the organic matter was discarded. Sample pH was adjusted to 10-11. 0.5 mL of 4% 2,4 dinitrofluorobenzene in ethanol was added. After shaking, 20 mL of deionized water and 10 g sodium chloride. The sample was then extracted 4 times with 30 mL chloroform. Sample was then dried and diluted in acetonitrile for analysis.
The recovery rate by the above operation was 86.3%.

Water samples were mixed with sodium chloride and extracted with 30 mL chloroform. Samples were dried and rediluted in 10 mL chloroform for analysis. The recovery rate by the above operation was 91-95%.
Hydrogenated castor oil + olive oil + rock sugar
Details on preparation of test solutions, spiked fish food or sediment:
10g of rock sugar was added to 1g of the tested substance, fully mixed and pulverized in a grinding machine, HCO-20 20g and olive oil 10g were then added and mixed. Deionized water was added to this and fixed to a total volume of 1L, preparing a 1000ppm (w/v) dispersion. For convenience, all concentration displays are shown treating the purity of the tested substance to be 100%, and no purity corrections were performed.
Test organisms (species):
Cyprinus carpio
Details on test organisms:
- Species: Cyprinus carpio
- Mean weight: 25.6 g
- Mean length: 9.8 cm
- Mean fat content: 4.9% (Bligh and Dyer, 1959)
Route of exposure:
Justification for method:
aqueous exposure method used for following reason: Study from 1982.
Test type:
Water / sediment media type:
natural water: freshwater
Test temperature:
Details on test conditions:
- Test vessel: tank
- Material: glass
- Volume: 100 L
- Method of exposure: flow-through
- Flow rate: 582 L/day

- Acclimation period: 14 days at 25°C (test temperature)
Nominal and measured concentrations:
Nominal: 500 µg/L and 50 µg/L
Measured for the 500 µg/L group: 503 µg/L (2 weeks) ; 500 µg/L (4 weeks) ; 496 µg/L (6 weeks) ; 480 µg/L (8 weeks)
Measured for the 50 µg/L group: 48.8 µg/L (2 weeks) ; 51.0 µg/L (4 weeks) ; 51.0 µg/L (6 weeks) ; 50.6 µg/L (8 weeks)
Reference substance (positive control):
Lipid content:
4.8 %
Time point:
start of exposure
Key result
<= 32 L/kg
not specified
Validity criteria fulfilled:
The BCF of the test substance was lower than 32 L/Kg whole body weight.
Executive summary:

Bioconcentration of dipentamethylenethiuram tetrasulfide was tested on Cyprinus carpio according a protocol similar to OECD 305 TG. The test involved two test concentrations (500 and 50 µg/L) under flow-through conditions. Test duration was 8 weeks. In both groups, BCFs were lower than 32 L/kg. This means that the degradation products of dipentamethylenethiuram hexasulfide with strictly less than 5 sulfides do not have a high potential for bioaccumulation.

Description of key information

Key value for chemical safety assessment

BCF (aquatic species):
32 L/kg ww

Additional information

The bioconcentration factor (BCF) of dipentamethylene thiuram hexasulphide is unknown. However, the BCF of dipentamethylenethiuram tetrasulfide was shown to be lower than 32 L/kg in a bioconcentration in fish study.

Therefore, it can be conclude that the smallest degradation products of DPTH are not B/vB and thus, not PBT/vPvB.

Additional information are available in the position paper attached to the IUCLID dossier.