Registration Dossier
Registration Dossier
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EC number: 940-422-0 | CAS number: -
Toxicity to terrestrial plants
Administrative data
- Endpoint:
- toxicity to terrestrial plants: long-term
- Type of information:
- migrated information: read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Study period:
- 2014-06-04 to 2014-06-25
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP study, no deficiencies
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 014
- Report date:
- 2014
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 208 (Terrestrial Plants Test: Seedling Emergence and Seedling Growth Test)
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- 940-373-5
- EC Number:
- 940-373-5
- IUPAC Name:
- 940-373-5
- Test material form:
- other: waxy solid
- Details on test material:
- - Name of test material (as cited in study report): C18/C18 unsatd.-Glucamide
- Physical state: Yellowish waxy solid
- Analytical purity: 100 %
- Sum formula: C25H49NO6 (C18 unsatd.-Glucamide) and C25H51NO6 (C18 satd.-Glucamide)
- Molecular weight:
C18 unsatd.-Glucamide: 459.67 g/Mol
C18 satd.-Glucamide 461.68 g/Mol
- Storage condition of test material: Room temperature, protected from light, in original container.
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
- Details on sampling:
- No analytical monitoring
Test substrate
- Vehicle:
- no
- Details on preparation and application of test substrate:
- - Method of mixing into soil (if used):The control and test item treatments were applied once at the beginning of the study.
Soil amount per test concentration and control: 16.86 kg, divided into two parts, each 8.43 kg
The respective test item amount was weighed out for each test item concentration, demineralised water was added and stirred at approximately 50 °C for 1 hour on a magnetic stirrer. Afterwards the test item solution and an appropriate amount of demineralised water (adjusting the soil to a moisture of 50 % of the maximum water holding capacity (WHC)) was added to the soil. Subsequently the soil was mixed carefully with the soil using a mixer to ensure a homogeneous distribution. The treated soil was transferred to the test container and the seeds were sown
- Control: Untreated soil
Test organisms
open allclose all
- Species:
- Brassica napus
- Plant group:
- Dicotyledonae (dicots)
- Details on test organisms:
- - Common name: Rape
- Plant family: Brassicaceae
- Variety: Kadore
- Source of seed: KWS Mais GmbH, D-37555 Einbeck, Germany
- Prior seed treatment/sterilization: No
- Seed storage: The seeds were stored dark at the test facility at room temperature (22 ± 10 °C) and protected from moisture until use.
- Species:
- Avena sativa
- Plant group:
- Monocotyledonae (monocots)
- Details on test organisms:
- - Common name: Oats
- Plant family: Gramineae
- Variety: Ivory
- Source of seed: NORDSAAT SAATZUCHT GMBH, 38895 Langenstein, Germany
- Prior seed treatment/sterilization: No
- Seed storage: The seeds were stored dark at the test facility at room temperature (22 ± 10 °C) and protected from moisture until use.
- Species:
- Glycine max (G. soja)
- Plant group:
- Dicotyledonae (dicots)
- Details on test organisms:
- - Common name: Soybean
- Plant family: Leguminosae
- Variety: Merlin
- Source of seed: Saatbau Linz, A-4021 Linz, Austria
- Prior seed treatment/sterilization: No
- Seed storage: The seeds were stored dark at the test facility at room temperature (22 ± 10 °C) and protected from moisture until use.
Study design
- Test type:
- seedling emergence toxicity test
- Study type:
- laboratory study
- Substrate type:
- natural soil
- Limit test:
- no
- Total exposure duration:
- 21 d
Test conditions
- Test temperature:
- nominal: 22 +/- 10 °C
actual: 16 - 21 °C - pH:
- 5.48 +/- 0.03 (natural soil)
- Moisture:
- - relative humidity: nominal: 70 +/- 25 %, actual: 60 - 100 %
- Details on test conditions:
- TEST SYSTEM
- Testing facility: Dr.U.Noack-Laboratorien
- Test container (type, material, size): Plastic container (standard flower pots) with a diameter of ca. 12 cm
- Amount of soil:
Soil amount per test concentration and control: 16.86 kg, divided into two parts, each 8.43 kg
- Method of seeding: Test containers were filled each with the same amount of treated soil (see application technique) and seeding holes were made
with a seedling pistil. One seed was given in each hole and covered with soil. During cultivation the test containers were bottom watered and
fertilized as necessary.
- No. of seeds per container: 5
- No. of replicates per treatment group: 8
- No. of replicates per control: 8
SOURCE AND PROPERTIES OF SUBSTRATE (if soil): A 2:1 mixture of natural soil LUFA 2.2 (batch number: Sp2.21214) and quartz sand (12a) was used.
- Geographic location: Soil collected by LUFA Speyer (67346 Speyer, Germany), quartz sand collected by Dörentrup Quarz GmbH Co. KG (31089
Duingen, Germany)
- Pesticide use history at the collection site: No pesticide use for the last five years.
- Soil texture (if natural soil)
- grain size: - Soil taxonomic classification: loamy sand (IS)
- Soil classification system: German DIN
- Organic carbon (%): 0.60
- Storage (condition, duration): The soil has been stored at the test facility at green house conditions until use.
NUTRIENT MEDIUM (if used)
- Description:
Fertilizer: HAKAPHOS® SOFT SPEZIAL 16+8+22(+3) (0.5 ‰)
Origin: GRÜNES LANDHAUS, St. Godehard-Str. 23, 31139 Hildesheim, Germany
Nutrient Concentration [%]
Ammonium nitrogen 9.7
Nitrate nitrogen 6.3
P2O5 8
Potassium oxide 22
Magnesium oxide 3
Boron 0.03
Iron (Chelate) 0.15
Copper 0.02
Manganese 0.05
Molybdenum 0.005
Zinc 0.015
GROWTH CONDITIONS
- Photoperiod: 16 h
- Light source: Special-fluorescent tube Fluora and Daylight (OSRAM)
- Light intensity and quality: nominal: > 3500 lx, actual: 4524 ± 660
- Day/night temperatures: 22 +/- 10 °C, actual: 16 - 21 °C
- Relative humidity (%): 70 +/- 25, actual: 60 - 100
- Watering regime and schedules: Bottom watering of the test containers, including fertilizer as necessary.
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
During the 21 day observation period the plants were observed every 7 days for number of emerged seedlings, number of dead plants and visual phytotoxic effects. The rating of the treated plants was done in relation to the control plants. Observations included all variations, either inhibitory or stimulatory, between the treated test replicates and the untreated control replicates. Such variations were phytotoxic symptoms (chlorosis, necrosis, wilting), formative effects and growth and development rates. At the end of the study shoot height and shoot fresh weight were measured.
- Phytotoxicity rating system (if used):
(1) Alteration of form and appearance (deformation)
(1.1) Curling / twisting / sticking or other deformation of the leaves
(1.2) Change of appearance
(1.3) Shoot deformations
(1.4) Wilting
(1.5) Leaf shedding
(1.6) Shedding of the leaf apex
(1.7) Stunted growth
(2) Discolouration of the leaves
(2.1) Lighter green
(2.2) Darker green
(2.3) Chlorosis
(2.3.1) Spots
(2.3.2) Whole leaf
(2.3.3) Veins
(2.3.4) Between veins
(2.3.5) Marginal
(2.4) Loss of colour
(2.5) Discolouration
(2.6) Discoloration of the veins
(2.7) Discoloration of the shoot
(3) Alteration of the leaf cells
(3.1) Necrosis
(3.1.1) Spots
(3.1.2) Marginal
(3.1.3) Apical
(3.1.4) Between veins
(3.1.5) Whole leaf
(3.1.6) Veins
(E) Dead plant (Number of dead plants)
Assessment of the Effects
- = no effects observed / normal growth
+/- = slight effects
+ = medium effects
++ = strong effects
VEHICLE CONTROL PERFORMED: no
TEST CONCENTRATIONS
- Range finding study
Results of the Preliminary Test: Inhibition of Shoot Fresh Weight and Emergence on day 21
Test
concentration Inhibition of shoot fresh weight [%] Inhibition of emergence
[%]
[mg/kg DW] Oats Rape Soybean Oats Rape Soybean
10 -15 -10 7 -5 -5 0
100 -13 1 7 -5 -5 0
500 2 12 11 0 0 0
1000 10 54 26 -5 0 5
- Nominal and measured concentrations:
- nominal:
1000 - 500 - 250 - 125 - 62.5 mg/kg DW (dry weight) - Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Species:
- Avena sativa
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 500 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth
- Remarks:
- (Shoot fresh weight)
- Species:
- Avena sativa
- Duration:
- 21 d
- Dose descriptor:
- EC10
- Effect conc.:
- 706 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth
- Remarks:
- (Shoot fresh weight)
- Species:
- Avena sativa
- Duration:
- 21 d
- Dose descriptor:
- EC25
- Effect conc.:
- 987 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth
- Remarks:
- (Shoot fresh weight)
- Remarks on result:
- other: 845 - > 1000 mg/kg soil dw
- Species:
- Avena sativa
- Duration:
- 21 d
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth
- Remarks:
- (Shoot fresh weight)
- Species:
- Brassica napus
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 250 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth
- Remarks:
- (Shoot fresh weight)
- Species:
- Brassica napus
- Duration:
- 21 d
- Dose descriptor:
- EC10
- Effect conc.:
- 366 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth
- Remarks:
- (Shoot fresh weight)
- Species:
- Brassica napus
- Duration:
- 21 d
- Dose descriptor:
- EC25
- Effect conc.:
- 579 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth
- Remarks:
- (Shoot fresh weight)
- Remarks on result:
- other: 448 - 771 mg/kg soil dw
- Species:
- Brassica napus
- Duration:
- 21 d
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth
- Remarks:
- (Shoot fresh weight)
- Species:
- Glycine max (G. soja)
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 500 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth
- Remarks:
- (Shoot fresh weight)
- Species:
- Glycine max (G. soja)
- Duration:
- 21 d
- Dose descriptor:
- EC10
- Effect conc.:
- 692 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth
- Remarks:
- (Shoot fresh weight)
- Species:
- Glycine max (G. soja)
- Duration:
- 21 d
- Dose descriptor:
- EC25
- Effect conc.:
- > 1 000 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth
- Remarks:
- (Shoot fresh weight)
- Species:
- Avena sativa
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 1 000 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth
- Remarks:
- (Shoot height)
- Species:
- Avena sativa
- Duration:
- 21 d
- Dose descriptor:
- EC10
- Effect conc.:
- > 1 000 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth
- Remarks:
- (Shoot height)
- Species:
- Brassica napus
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 1 000 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth
- Remarks:
- (Shoot height)
- Species:
- Brassica napus
- Duration:
- 21 d
- Dose descriptor:
- EC10
- Effect conc.:
- > 1 000 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth
- Remarks:
- (Shoot height)
- Species:
- Glycine max (G. soja)
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 1 000 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth
- Remarks:
- (Shoot height)
- Species:
- Glycine max (G. soja)
- Duration:
- 21 d
- Dose descriptor:
- EC10
- Effect conc.:
- > 1 000 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth
- Remarks:
- (Shoot height)
- Species:
- Avena sativa
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 1 000 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- seedling emergence
- Species:
- Avena sativa
- Duration:
- 21 d
- Dose descriptor:
- EC10
- Effect conc.:
- > 1 000 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- seedling emergence
- Species:
- Brassica napus
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 1 000 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- seedling emergence
- Species:
- Brassica napus
- Duration:
- 21 d
- Dose descriptor:
- EC10
- Effect conc.:
- > 1 000 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- seedling emergence
- Species:
- Glycine max (G. soja)
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 1 000 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- seedling emergence
- Species:
- Glycine max (G. soja)
- Duration:
- 21 d
- Dose descriptor:
- EC10
- Effect conc.:
- > 1 000 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- seedling emergence
- Details on results:
-
SEEDLING EMERGENCE
The percent inhibition of shoot height, shoot fresh weight and rate of emergence is given in the tables below. The tabulated results represent
rounded values calculated on the exact raw data.
Oats: Inhibition [%] of Shoot Height, Shoot Fresh Weight and Rate of Emergence
Test concentration
[mg/kg DW] Shoot Inhibition Shoot fresh Inhibition Rate of Inhibition
height weight emergence
[cm] [%] [mg] [%] [%] [%]
Control 40.8 - 978 - 90 -
62.5 40.0 2 997 -2 100 -11
125 40.4 1 997 -2 95 -6
250 39.1 4 1052 -8 98 -9
500 40.5 1 1002 -2 93 -3
1000 40.5 1 715 27 93 -3
negative values = promoted growth bold = statistically significant inhibition
Rape: Inhibition [%] of Shoot Height, Shoot Fresh Weight and Rate of Emergence
Test concentration
[mg/kg DW] Shoot Inhibition Shoot fresh Inhibition Rate of Inhibition
height weight emergence
[cm] [%] [mg] [%] [%] [%]
Control 12.3 - 1144 - 88 -
62.5 12.5 -2 1210 -6 85 3
125 12.4 -1 1149 0 90 -2
250 12.5 -2 1152 -1 90 -2
500 11.8 4 899 21 98 -11
1000 11.3 8 692 40 80 9
Soybean: Inhibition [%] of Shoot Height, Shoot Fresh Weight and Rate of Emergence
Test concentration
[mg/kg DW] Shoot Inhibition Shoot fresh Inhibition Rate of Inhibition
height weight emergence
[cm] [%] [mg] [%] [%] [%]
Control 23.4 - 1851 - 90 -
62.5 25.9 -11 2022 -9 98 -9
125 25.5 -9 1837 1 98 -9
250 25.2 -8 1791 3 100 -11
500 25.0 -7 1757 5 98 -9
1000 25.1 -7 1598 14 95 -6
Negative values = promoted growth - Results with reference substance (positive control):
- No reference substance tested
- Reported statistics and error estimates:
- The percent inhibition of biomass growth (shoot height, shoot fresh weight) and rate of emergence were calculated in relation to the control.
One Way Analysis of Variance (ANOVA) was carried out for the determination of statistically significant differences compared to control replicates. When
running a One Way Analysis of Variance a Normality test and an Equal Variance test were done first. P-values for both Normality and Equal Variance test are
0.05. The -value for ANOVA (acceptable probability of incorrectly concluding that there is a difference) is =0.05.
EC10, EC25 and EC50 values were calculated by sigmoidal dose-response regression. Calculation of the confidence intervals for EC-values was carried out using standard procedures.
Any other information on results incl. tables
Visual Phytotoxicity on Day 21
Oats: Visual Observations and Number of Dead Plants on Day 21
Test |
Phyto- |
Phytotoxic effects |
|||||||
Replicate |
|||||||||
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
||
control |
– |
- |
- |
- |
- |
- |
- |
- |
- |
62.5 |
– |
- |
- |
- |
- |
- |
- |
- |
- |
125 |
– |
- |
- |
- |
- |
- |
- |
- |
- |
250 |
– |
- |
- |
- |
- |
- |
- |
- |
- |
500 |
– |
- |
- |
- |
- |
- |
- |
- |
- |
1000 |
– |
- |
- |
- |
- |
- |
- |
- |
- |
Rape: Visual Observations and Number of Dead Plants on Day 21
Test |
Phyto- |
Phytotoxic effects |
|||||||
Replicate |
|||||||||
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
||
control |
– |
- |
- |
- |
- |
- |
- |
- |
- |
62.5 |
– |
- |
- |
- |
- |
- |
- |
- |
- |
125 |
– |
- |
- |
- |
- |
- |
- |
- |
- |
250 |
(3.1.5) |
- |
+/- |
- |
- |
- |
- |
- |
- |
500 |
(1.7) |
- |
+ |
- |
- |
+/- |
- |
+/- |
- |
|
(3.1.5) |
- |
+/- |
- |
- |
- |
- |
- |
- |
1000 |
– |
- |
- |
- |
- |
- |
- |
- |
- |
Soybean: Visual Observations and Number of Dead Plants on Day 21
Test |
Phyto- |
Phytotoxic effects |
|||||||
Replicate |
|||||||||
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
||
control |
– |
- |
- |
- |
- |
- |
- |
- |
- |
62.5 |
– |
- |
- |
- |
- |
- |
- |
- |
- |
125 |
– |
- |
- |
- |
- |
- |
- |
- |
- |
250 |
– |
- |
- |
- |
- |
- |
- |
- |
- |
500 |
– |
- |
- |
- |
- |
- |
- |
- |
- |
1000 |
– |
- |
- |
- |
- |
- |
- |
- |
- |
- = no effects observed / normal growth
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- Potential toxic effects of the test item were assessed on day 7, 14 and 21 by visual observations (phytotoxic effects and number of dead plants) and on day 21 by shoot height and shoot fresh weight determination. After 21 days no test item related phytotoxic effects were observed for all tested plant species at test concentrations up to 1000 mg/kg DW.
The NOEC values as well as the calculated EC-values with confidence ranges are given in the tables below.
Shoot Height: NOEC, EC10-, EC25- and EC50- Values with Confidence Range (p)
Species NOEC EC10 EC25 p = 95% EC50 p = 95%
[mg/kg DW] [mg/kg DW] [mg/kg DW] [mg/kg DW] [mg/kg DW] [mg/kg DW]
Oats 1000 > 1000 > 1000 – > 1000 –
Rape 1000 > 1000 > 1000 – > 1000 –
Soybean 1000 > 1000 > 1000 – > 1000 –
-) Not determinable
Shoot Fresh Weight: NOEC, EC10-, EC25- and EC50- Values with Confidence Range (p)
Species NOEC EC10 EC25 p = 95% EC50 p = 95%
[mg/kg DW] [mg/kg DW] [mg/kg DW] [mg/kg DW] [mg/kg DW] [mg/kg DW]
Oats 500 706 987 845 - > 1000 > 1000 –
Rape 250 366 579 448 - 771 > 1000 –
Soybean 500 692 > 1000 – > 1000 –
Rate of Emergence: NOEC, EC10-, EC25- and EC50- Values with Confidence Range (p)
Species NOEC EC10 EC25 p = 95% EC50 p = 95%
[mg/kg DW] [mg/kg DW] [mg/kg DW] [mg/kg DW] [mg/kg DW] [mg/kg DW]
Oats 1000 > 1000 > 1000 – > 1000 –
Rape 1000 > 1000 > 1000 – > 1000 –
Soybean 1000 > 1000 > 1000 – > 1000 –
- Executive summary:
The phytotoxicity of the test item C18/C18 unsatd.-Glucamide (batch number:SN119/13/3-8) to three terrestrial plant species was determined in a seedling emergence test over a period of 21 days, from 2014 -06 -04 to 2014 -06 -25 according to OECD 208 (July 2006) at Dr.U.Noack-Laboratorien, 31157Sarstedt, Germany.
Test systems were one monocotyledon, oats (Gramineae), and two dicotyledons, rape (Brassicaceae) and soybean (Leguminosae).
The test was conducted with the test concentrations1000 - 500 - 250 - 125 -62.5 mg/kg DW (factor 2).
The test item was incorporated into the soil in which the seeds were sown afterwards. The test containers were bottom watered with nutrient solution as needed. The toxic effects of the test item were determined on day 7, 14 and 21 by visual observations (number of emerged seedlings and phytotoxic effects) and on day 21 by shoot height and shoot fresh weight determination.
After 21 days no test item related phytotoxic effects were determined for all tested plant species up to the test concentration 1000 mg/kg DW (section12.3.3).
The statistically calculated NOEC values, as well as thecalculated EC10-, EC25- and EC50-values, are summarized in the tables below.
The results of the control for all plant species met the required validity criteria. Therefore, the test is considered to be valid.
Shoot Height:NOEC, EC10-, EC25- and EC50- Values with Confidence Range (p)
Species
NOEC
[mg/kg DW]EC10
[mg/kg DW]EC25
[mg/kg DW]p = 95%
[mg/kg DW]EC50
[mg/kg DW]p = 95%
[mg/kg DW]Oats
1000
> 1000
> 1000
–
> 1000
–
Rape
1000
> 1000
> 1000
–
> 1000
–
Soybean
1000
> 1000
> 1000
–
> 1000
–
Shoot Fresh Weight:NOEC, EC10-, EC25- and EC50- Values with Confidence Range (p)
Species
NOEC
[mg/kg DW]EC10
[mg/kg DW]EC25
[mg/kg DW]p = 95%
[mg/kg DW]EC50
[mg/kg DW]p = 95%
[mg/kg DW]Oats
500
706
987
845 - > 1000
> 1000
–
Rape
250
366
579
448 - 771
> 1000
–
Soybean
500
692
> 1000
–
> 1000
–
Number of Emerged Seedlings: NOEC, EC10-, EC25- and EC50- Values with Confidence Range (p)
Species
NOEC
[mg/kg DW]EC10
[mg/kg DW]EC25
[mg/kg DW]p = 95%
[mg/kg DW]EC50
[mg/kg DW]p = 95%
[mg/kg DW]Oats
1000
> 1000
> 1000
–
> 1000
–
Rape
1000
> 1000
> 1000
–
> 1000
–
Soybean
1000
> 1000
> 1000
–
> 1000
–
-) Not determinable
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