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EC number: 201-906-0 | CAS number: 89-41-8
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1990
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: well performed guideline study with GLP
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 991
- Report date:
- 1991
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- GLP compliance:
- yes
Test material
- Reference substance name:
- 3-nitro-p-anisic acid
- EC Number:
- 201-906-0
- EC Name:
- 3-nitro-p-anisic acid
- Cas Number:
- 89-41-8
- Molecular formula:
- C8H7NO5
- IUPAC Name:
- 4-methoxy-3-nitrobenzoic acid
- Details on test material:
- purity: 98.2 %
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic (adaptation not specified)
- Details on inoculum:
- source: domestic waste water treatment plant Frankfurt-Sindlingen
- Duration of test (contact time):
- 28 d
Initial test substance concentrationopen allclose all
- Initial conc.:
- ca. 20 mg/L
- Based on:
- test mat.
- Initial conc.:
- ca. 10 mg/L
- Based on:
- test mat.
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- CO2 evolution
Reference substance
- Reference substance:
- benzoic acid, sodium salt
Results and discussion
% Degradationopen allclose all
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 3
- Sampling time:
- 3 d
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 7
- Sampling time:
- 7 d
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 10
- Sampling time:
- 14 d
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 14
- Sampling time:
- 20 d
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 31
- Sampling time:
- 28 d
BOD5 / COD results
- Results with reference substance:
- 78% degradation after 14 days
85% degradation after 20 days
94% degradation after 28 days
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- other: not readily biodegradable
- Conclusions:
- The substance is not readily biodegradable
- Executive summary:
The substance was tested for ready biodegradability according to OECD guideline 301 B (modified Sturm test). Based on the test results and the definition of "ready biodegradability" the substance has to be considered as not readily biodegradable.
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