Registration Dossier
Registration Dossier
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 283-907-6 | CAS number: 84775-78-0 Extractives and their physically modified derivatives such as tinctures, concretes, absolutes, essential oils, oleoresins, terpenes, terpene-free fractions, distillates, residues, etc., obtained from Ascophyllum nodosum, Fucaceae.
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Toxicity to terrestrial arthropods
Administrative data
- Endpoint:
- toxicity to terrestrial arthropods: short-term
- Type of information:
- migrated information: read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Study period:
- July - September 2010
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: in accordance with guidelines
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 010
- Report date:
- 2010
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- other: ESCORT I Guidance Document (Barret et al., 1994)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- other: ESCORT II Guidance Document (Candolfi et al., 2000)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- other: IOBC Guidelines (Mead-Briggs et al., 2000)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- other: guideline of the ring testing group (Mead-Briggs et al., 2009)
- Deviations:
- no
- Principles of method if other than guideline:
- SOP BT207 , Biotecnologie BT internal procedure.
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- Italian Ministero della Salute
- Application method:
- spray
Test material
- Reference substance name:
- Ascophyllum nodosum, ext.
- EC Number:
- 283-907-6
- EC Name:
- Ascophyllum nodosum, ext.
- IUPAC Name:
- 283-907-6
- Details on test material:
- - Name of test material : ALGEA FERT SOLID K+
- Substance type: algal extract, intended to be used as agriculture fertilizer
- Physical state: solid, balck micro-flakes
- Lot/batch No.: 00680
- Expiration date of the lot/batch: 09 March 2012
- Stability under test conditions: stable
Constituent 1
Test substrate
- Details on preparation and application of test substrate:
- The applications were performed by a spraying equipment, calibrated to deliver spray at volume rate equivalent to 200 L/ha. Simultaneously other glass plates were sprayed with deionised water for the control and with Perfekthion for the reference substance group.
After the application of the spray solutions, when the treated plates were dried, the exposure units were assembled.
After 48 hours of exposure to the treated glass plates, the surviving females were removed with an aspirator from the exposure cage and they were taken apart. Each female (15 females for the treatment group and 15 females for the control group) was transferred to single fecundity cage, and given 24 hour time period to parasitize aphids. In the fecundity cages there were untreated Aphids infested plants. After 24 hours the females were removed from the fecundity cages and their condition (alive, dead or not recovered) was recorded. The parasitized aphids within the fecundity arenas were left to develop in situ and the number of aphid “mummies” that developed was recorded 17 days later.
Test organisms
- Test organisms (species):
- Aphidius rhopalosiphi
- Animal group:
- Hymenoptera (leaf-dwelling parasitic wasp)
- Details on test organisms:
- TEST ORGANISM
- Common name: Aphidius rhopalosiphi De Stefani Perez
- Source: Biotecnologie BT S.r.l.
- Age at test initiation : Adults
- Disease free: yes
- Kept according to standard practices: yes
- Acclimation period: not relevant
- Feeding: Water/honey solution (During the exposure phase, a piece of a cotton wool soaked with a 1:3 v/v solution of honey and water was inserted into the access hole as a source of food and was re-moistened at least once a day. During the 24 h parasitisation period the parasitoids were fed on the honeydew of the aphids, which were offered for parasitisation).
Study design
- Study type:
- laboratory study
- Limit test:
- yes
- Total exposure duration:
- 18 d
- Post exposure observation period:
- The conditions of the exposed parasitoids were recorded at approximately 2, 24 and 48 hours after their introduction. Counting of parasitized aphids was carried out 17 days after the start of the fecundity test.
Test conditions
- Test temperature:
- exposure phase: 20.00 – 21.33°C / reproduction phase: 19.33 – 22.00°C
- Humidity:
- exposure phase: 68.50 – 77.00%
- Photoperiod and lighting:
- Photoperiod: 16 h light and 8 h darkness
Light Intensity: reproduction phase: 6800 - 9400 lux - Details on test conditions:
- TEST SYSTEM
- Test container / cage (material, size): The test system was exposed to the test item on circular glass plates (diameter: 10.00 cm), which were assembled to cages with an aluminium frame (diameter: 10.00 cm; height: 1.50 cm; width: 1.00 cm)after the spray layer has dried.
- No. of organisms per container (treatment): 10
- No. of replicates per treatment group: 5
- No. of replicates per control : 5
- No. of replicates per reference substance (Perfekthion): 5
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mortality and reproduction performance (at approximately 2, 24 and 48 hours after treatment). - Nominal and measured concentrations:
- One single rate equivalent to twice the maximum field application rate multiplied by the MAF (worst case exposure): 21000.00 Product g/ha
- Reference substance (positive control):
- yes
- Remarks:
- formulation Perfekthion
Results and discussion
Effect concentrations
- Duration:
- 48 h
- Dose descriptor:
- LR50
- Effect conc.:
- 21 000 g/ha
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Details on results:
- Mortality at end of exposure period: very low ( 14%)
- Results with reference substance (positive control):
- Mortalityat end of exposure period: 96%
- Reported statistics and error estimates:
- Appropriate statistical methods were used to analyse mortality and fecundity data for significance: Student’ T Test and Anova Manova Test.
Any other information on results incl. tables
Table 1: Mortality at 48 hours
Product |
g product/ha |
g a.s./ha |
% Mortality |
% Corrected Mortality |
Control |
0.00 |
0.00 |
6.00 |
=== |
ALGEA FERT SOLID K+ |
21000.00 |
n.a. |
14.00 |
8.51 |
Perfekthion |
10.00 |
3.65 |
96.00 |
95.74 |
Table 2. Reproduction performance
Product |
g product/ha |
g a.s./ha |
N° of mummies/♀ |
±SD |
% R |
Control |
0.00 |
0.00 |
9.80 |
7.29 |
=== |
ALGEA FERT SOLID K+ |
21000.00 |
n.a. |
6.33 |
6.48 |
35.37 |
%R = % reduction in reproduction
During the reproduction performance there was a reduction in reproduction of parasitoids at the tested concentration: this value was 35.37%. The mean for the tested concentration was not different to the control mean at alpha = 0.05, by the Student’s T Test (p = 0.1795).
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- In accordance with IOBC/WSPR guidance, the test is valid because the mortality observed in the untreated control group after 48 hours was ≤ 10.00% and the mortality caused by the reference item was ≥ 50%. Moreover the control parasitisation rate (mean) wa
- Conclusions:
- The results obtained in the trial showed that the test item ALGEA FERT SOLID K+ did not cause a statistically significant mortality of the test organism Aphidius rhopalosiphi De Stefani Perez (Hymenoptera: Braconidae) when applied to glass plates at twice the worst case application rate. At the rate tested, the corrected mortality was < 50%.
The LR50 of ALGEA FERT SOLID K+ calculated at 48 hours after treatment, is higher than 21.00 kg of product/ha.
During the reproduction performance the treated surviving females had a decrease in reproductive capacity equivalent to 35.37%. Than the ER50 value was > 21.00 kg/ha. - Executive summary:
The effects of residues of ALGEA FERT SOLID K+on the mortality and reproduction of the aphid parasitoidAphidius rhopalosiphiDe Stefani Perez (Hymenoptera: Braconidae), were tested with a laboratory study, according to GLP regulations. The test item was sprayed on glass plates with calibrated spray equipment () at one single rate equivalent to twice the maximum field application rate multiplied by the MAF (worst case exposure). The formulation Perfekthion was used as reference substance, whereas deionised water was used as control. Five replicates for each treatment were performed. The sprayed test substances were allowed to dry for about 1 hour, than in each cage 10 adults were introduced.After 48 hours of exposure to the treated glass plates the surviving parasitoids were removed from the exposure cages and the parasitic capacity per female was evaluated in the fecundity test. The conditions of the exposed parasitoids were recorded at approximately 2, 24 and 48 hours after their introduction. Counting of parasitized aphids was carried out 17 days after the start of the fecundity test. The results obtained in the trial showed that the test item ALGEA FERT SOLID K+ did not cause a statistically significant mortality of the test organismAphidius rhopalosiphiDe Stefani Perez (Hymenoptera: Braconidae) when applied to glass plates at twice the worst case application rate. At the rate tested, the corrected mortality was < 50%. The LR50of ALGEA FERT SOLID K+ calculated at 48 hours after treatment, is higher thanof product/ha.
During the reproduction performance the treated surviving females had a decrease in reproductive capacity equivalent to 35.37%. Than the ER50value was > 21.00 kg/ha.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.

EU Privacy Disclaimer
This website uses cookies to ensure you get the best experience on our websites.