2-vinylpyridine

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Remarks:

Type of genotoxicity: gene mutation

Type of information:

experimental study

Adequacy of study:

weight of evidence

Study period:

2000 - 2002.

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: A standard NTP (National Toxicology Program) protocol. Equivalent to OECD 471.

Data source

Reference

Reference Type:

publication

Title:

Unnamed

Year:

2002

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

other: Standard NTP Protocol.

Deviations:

no

Test guideline 2

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 471 (Bacterial Reverse Mutation Assay)

Deviations:

yes

Remarks:

(only three Salmonella strains were tested)

GLP compliance:

not specified

Type of assay:

bacterial reverse mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

2-vinylpyridine

EC Number:

202-879-8

EC Name:

2-vinylpyridine

Cas Number:

100-69-6

Molecular formula:

C7H7N

IUPAC Name:

2-ethenylpyridine

Details on test material:

- Name of test material (as cited in study report): 2-vinylpyridine

Method

Species / strain

Species / strain / cell type:

S. typhimurium, other: S. typhimurium TA 100, TA 1535 and TA 98.

Metabolic activation:

with and without

Metabolic activation system:

Induced male Sprague Dawley rat liver S9 mix and induced male Syrian hamster liver S9 mix.

Test concentrations with justification for top dose:

TA 1535: 0, 100, 333, 1000, 2000, 3333, 4000 μg/plate.
TA 98 and TA 100: 0, 100, 333, 1000, 2000, 3333, 5000, 10000 μg/plate.

Vehicle / solvent:

- Vehicle/solvent used: Dimethyl Sulfoxide.

Controls

Untreated negative controls:

no

Negative solvent / vehicle controls:

yes

Remarks:

Dimethyl Sulfoxide.

Positive controls:

yes

Positive control substance:

sodium azide

other: 2-aminoanthracene, 4-nitro-o-phenylenediamine.

Remarks:

(sodium azide: without metabolic activation; 2-aminoanthracene, 4-nitro-o-phenylenediamine: with metabolic activation).

Details on test system and experimental conditions:

METHOD OF APPLICATION: preincubation method.

DURATION
- Preincubation period: 20 minutes at 37 ºC.

Results and discussion

Test results

Test results 1

Species / strain:

S. typhimurium, other: S. typhimurium TA 100, TA 1535 and TA 98.

Metabolic activation:

without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

cytotoxicity

Remarks:

(slightly toxicity at ≥ 10000 µg/plate)

Vehicle controls validity:

valid

Untreated negative controls validity:

not applicable

Positive controls validity:

valid

Test results 2

Species / strain:

S. typhimurium, other: S. typhimurium TA 100 and TA 1535.

Metabolic activation:

with

Genotoxicity:

positive

Cytotoxicity / choice of top concentrations:

cytotoxicity

Remarks:

(slightly toxicity at ≥ 5000 µg/plate)

Vehicle controls validity:

valid

Untreated negative controls validity:

not applicable

Positive controls validity:

valid

Test results 3

Species / strain:

S. typhimurium TA 98

Metabolic activation:

with

Genotoxicity:

ambiguous

Cytotoxicity / choice of top concentrations:

cytotoxicity

Remarks:

(slightly toxicity at ≥ 5000 µg/plate)

Vehicle controls validity:

valid

Untreated negative controls validity:

not applicable

Positive controls validity:

valid

Remarks on result:

other: all strains/cell types tested

Remarks:

Migrated from field 'Test system'.

Applicant's summary and conclusion

Conclusions:

Interpretation of results (migrated information):
positive with metabolic activation (induced male Syrian hamster liver S9).

The test substance is considered to be genotoxic in vitro.

Executive summary:

A standard NTP (National Toxicology Program) protocol was performed to investigate the mutagenicity of 2-vinylpyridine (test method equivalent to OECD 471). Different concentrations of the test substance were tested in Salmonella typhimurium strains TA 100, TA 1535 and TA 98 by the preincubation assay (37 ºC, 20 min) with and without metabolic activation (induced male Syrian hamster liver S9 mix and induced male SD rat liver S9 mix). Positive and negative controls confirmed the sensitivity of the test system. In the presence of exogenous metabolic activation, Salmonella strains TA 100 and TA 1535 gave positive results, while S. typhimurium TA 98 gave ambiguous results. Slightly toxicity was found at concentrations of 5000 µg/plate and above. From the results of the present study, the test substance is considered to be genotoxic in vitro.