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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Endpoint summary

Administrative data

Description of key information

Additional information

The 24, 48, 72 and 96 hour median lethal concentrations (LC50) of PTH-Decahydroamide to the freshwater fish, rainbow trout (Oncorhynchus mykiss previously known as Salmo gairdneri) were determined under static test conditions at a temperature of 15 ± 1 °C. The 96 hour LC50 = 68 mg PTH-Decahydroamide/litre with 95% confidence intervals of 57 to 84 mg/L. The highest test concentration causing no mortality within the period of the test was 32 mg/L. The lowest that caused 100% mortality within the period of the test was 100 mg/L.

The 24 and 48 hour EC50 values of PTH-Decahydroamid to Daphnia magna were determined under static test conditions at a temperature of 20 ± 1 °C. The 48 hour EC50 was 45 mg PTH-Decahydroamid/litre with 95% confidence intervals of 33 to 65 mg/L. The highest nominal test concentration causing no immobility was 5.6 mg/L and the lowest that caused 100% immobility was 180 mg/L.

The toxicity of the test substance to aquatic algae was determined using OECD method 201. It was determined that PTH-Decahydroamide (Ro 31-9373/000) inhibited cell growth and reduced growth rate of Scenedesmus subspicatus significantly under the conditions of the present study at nominally 10 mg/l and higher. The inhibition rates were estimated for both the algal biomass b and the growth rate R after 72 hours test duration; based on nominal concentrations.

EbC50 (0-72 h)                     46 mg/l

ERC50 (0-72 h)                     74 mg/l

The NOEC was 4.8 mg/l for both cell growth inhibition and growth rate reduction when based on nominal concentrations. Since this concentration was below the detection limit of PTH-decahydroamide (Ro 31-9373/000) in water samples, no actual concentration could be determined.

No significant inhibition in respiration rate of the sludge (> 10 %) was recorded at 100 mg PTH-decahydroamide (Ro 31-9373/000) per litre. Thus under the conditions of the test PTH-decahydroamide (Ro 31-9373/ 000) was not toxic to waste water bacteria.