2-Butenoic acid, 3-amino-, 2-methylpropyl ester

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

other information

Study period:

1988

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Reliable study performed according to scientific standards

Data source

Reference

Reference 1

Reference Type:

study report

Title:

Unnamed

Year:

1985

Report date:

1985

Reference 2

Reference Type:

publication

Title:

Acute Toxicologic Evaluation of 3-Amino-2-butenoic acid isobutylester

Author:

Bomhard E, Ramm W

Bibliographic source:

Int. J. Toxicol. 19 337, 2000

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: Directive 84/449/EWG (Amtsblatt der Europäischen Gemeinschaften Nr. L 251 vom 19.09.1984, S. 96)

GLP compliance:

no

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

isobutyl (2Z)-3-aminobut-2-enoate

EC Number:

610-915-2

Cas Number:

52937-90-3

Molecular formula:

C8 H15 N O2

IUPAC Name:

isobutyl (2Z)-3-aminobut-2-enoate

Test animals

Species:

rat

Strain:

Wistar

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Harlan Winkelmann, Borchen, Germany
- Strain: Bor:WISW (SPF Cpb)
- Age at study initiation: adult mean (173 g males - 175 g females)
- Diet: ad libitum
- Water: ad libitum

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

other: Lutrol (PEG 400)

Details on oral exposure:

- Application volume: 20 mL/kg bw

Doses:

1000, 1300, 1600, 2000, and 2500 mg/kg bw

No. of animals per sex per dose:

5

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: at least once daily (clinical signs, mortality) or once weekly (weight gain)
- Necropsy of survivors performed: yes

Statistics:

Rosiello et al, J. Tox and Environ. Health 3, 797-809, 1977

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

1 400 mg/kg bw

Based on:

test mat.

Mortality:

1000 mg/kg bw: 0/5 males, 0/5 females
1300 mg/kg bw: 3/5 males, 2/5 females
1600 mg/kg bw: 4/5 males, 2/5 females
2000 mg/kg bw: 4/5 males, 4/5 females
2500 mg/kg bw: 5/5 males, 5/5 females

Clinical signs:

other: doses of 1300 mg/kg and above: poor general condition, dazed condition, abdominal or lateral position, piloerection; on day 4 signs had disappeared

Gross pathology:

At necropsy dead animals showed loss of gastric mucosal relief, the stomach fundus and the intestines were reddened; final necropsy at day 14 revealed no abnormalities.

Applicant's summary and conclusion

Interpretation of results:

harmful

Remarks:

Migrated information

Executive summary:

The acute oral toxicity of 3 -Aminocrotonsäurebutylester was determined with an LD50 value of 1400 mg/kg bw in rats. Doses of 1000 up to 2500 mg/kg bw were tested in groups of 5 male and 5 female rats. Mortality occurred at doses of 1300 mg/kg bw and above. All animals in the 2500 mg/kg bw group died. Thus, the test item should be considered as harmful after oral uptake.