Hydrothermal synthesis product of calcium oxide, quartz and water

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1971

Report date:

1971

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Silicic acid, calcium salt

EC Number:

215-710-8

EC Name:

Silicic acid, calcium salt

Cas Number:

1344-95-2

IUPAC Name:

Silicic acid, Calcium salt

Test material form:

solid

Specific details on test material used for the study:

- Synthetic amorphous silicas
- Analytical data after ignition: SiO2 (ca. 79 %); CaO (ca. 7%); NaCl (ca. 1.2 %)

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: direct addition and stirring (1000 mg/L) or as acetonic suspension (1 and 10 % silicate in solution, w/v) (see Report, Tab. I)
- Eluate: no, used as dispersion/suspension
- Differential loading: yes
- Controls: vehicle control not explicitly indicated to
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): acetone for 1, 10, and 100 mg/L
- Concentration of vehicle in test medium (stock solution and final test solution(s) including control(s):
0.1 mL acetone/L at 1 and 10 mg silicate/L
1 mL acetone/L at 100 mg silicate/L
0 mL acetone/L at 1000 mg silicate/L

- Evidence of undissolved material (e.g. precipitate, surface film, etc): yes

Test organisms

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Details on test organisms:

TEST ORGANISM
- Source: no data
- Age at study initiation: no data (fingerlings)
- Length at study initiation: range 35 - 75 mm
- Weight at study initiation (mean and range, SD): no data
- Method of breeding: no data
- Feeding during test: no


ACCLIMATION
- Acclimation period: >= 14 d before testing, 24 h in test vessel before addition of the test material
- Acclimation conditions (same as test or not): yes
- Type and amount of food: Purina Trout Chow
- Feeding frequency: until 3 d prior to testing

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Post exposure observation period:

none

Test conditions

Test temperature:

13 °C (15 ± 2 °C)

pH:

control: 6.9 - 7.3;
tests: 7.2 - 7.3 (only measured in case of mortality)

Dissolved oxygen:

control: 4.3 - 7.4 mg/L;
tests: 5.6 - 8.2 mg/L (only measured in case of mortality)

Nominal and measured concentrations:

0, 1, 10, 100, and 1000 mg/L [ppm] (nominal)

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): open
- Material, fill volume: Vessel lined with a disposable PE bags, 12.5 L
- Aeration: none
- Renewal rate of test solution (frequency/flow rate): none
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- No. of vessels per vehicle control (replicates): no data
- Biomass loading rate: no data


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted water prepared from deionized water
30 mg CaSO4/L; 30 mg MgSO4/L; 48 mg NaHCO3/L; 2 mg KCl/L
- Culture medium different from test medium: no


OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: no data


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mortality and behaviour


TEST CONCENTRATIONS
- Spacing factor for test concentrations: 10
-
- Justification for using less concentrations than requested by guideline:
- Range finding study: done
- Test concentrations: no data
- Results used to determine the conditions for the definitive study: no data

Reference substance (positive control):

yes

Remarks:

Toxaphene

Results and discussion

Effect concentrations

Key result

Duration:

96 h

Dose descriptor:

LL50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

- Behavioural abnormalities: at 1 - 100 mg/L, no particular findings
at 1000 mg/L, quiescent behaviour
- Observations on body length and weight: no data
- Other biological observations: none
- Mortality of control: none
- Other adverse effects control: none
- Abnormal responses: at 1000 mg/L
- Effect concentrations exceeding solubility of substance in test medium: yes

Results with reference substance (positive control):

- Results with reference substance valid? yes

- Mortality: 0.01 0.018 0.056 mg/L
===================
1/10 3/10 10/10
----------------------------

- LC50(96 h): 0.023 mg/L

Reported statistics and error estimates:

LD50 determined according to Litchfield and Wilcoxon 1949

Any other information on results incl. tables

Sublethal observations / clinical signs:

Mortality under test conditions: Calcium silicate (data taken and derived from Report, Tab. I):

Concentration		Mortality
(nominal, mg/L)	%*	24 h	48 h	72 h	96 h
Control	-	0/10	0/10	0/10	0/10
1	1	0/10	0/10	0/10	0/10
10	10	0/10	0/10	0/10	0/10
100	10	0/10	0/10	1/10	1/10
1000	100	0/10	1/10	1/10	2/10

* The silicate was dispensed into the test vessel in undiluted form or as a 1% or 10% (w/v) solution in acetone.