2-butyryl-3-hydroxy-5-thiocyclohexan-3-yl-cyclohex-2-en-1-one

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study with analytical monitoring

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1996

Report date:

1996

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

-

EC Number:

425-150-8

EC Name:

-

Cas Number:

94723-86-1

Molecular formula:

C15 H22 O3 S

IUPAC Name:

2-butanoyl-3-hydroxy-5-(thian-3-yl)cyclohex-2-en-1-one

Details on test material:

- Name of test material (as cited in study report): 625 - Triketone
- Physical state: solid
- Analytical purity: 94%
- Impurities (identity and concentrations): diketone, water
- Lot/batch No.: LJ 27 881/1

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

- The following nominal concentrations were analysed: control, 1.56, 12.5, 100 mg/L
- At the start of the test (after 0 h) samples from vessels which were not stocked with daphnids were analysed and at the end of the test (after 48 h) samples from stocked and unstocked vessels were analysed.

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: At the beginning of the test the stock solution of the test substance was freshly prepared. A defined amount of the substance was weighed out and suspended directly in the test medium and stirred for about 20 h at 20 °C. Afterwards small amounts of undissolved particles were removed by means of centrifugation (20 min. at about 7000G). The test was performed with the supernantant.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: watre flea
- Source: supplied by Institut National de Recherche Chimique Appliquée, France, in 1978 and cultured under standard contiotions in the laboratory
- Age at study initiation: 2- 24 h
- Feeding during test: none

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Hardness:

2.47 mmol/L

Test temperature:

19.8 - 21.3 °C

pH:

7.9 - 8.0

Dissolved oxygen:

8.2 - 8.5

Nominal and measured concentrations:

Nominal test concentrations: control, 1.56, 3.13, 6.25, 12.5, 25, 50, 100 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Material, nominal volume, fill volume: test tubes (glass) with flat bottom, 20 ml, 10 ml
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Biomass loading rate: 0.5 animals/ml

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Synthetic medium M4 is used for the culture and test. The medium is prepared on the basis of an ultrapure, deionized water (conductivity 0.05 µS/cm).
- Alkalinity: 0.84 mmol/L up to pH 4.3
- Conductivity: 636 µS/cm
- Culture medium different from test medium: no
- Intervals of water quality measurement: after 0, 24,48 h in an extra vessel close to the test vessel, After 0 and 48 h in one replicate at each concentration

OTHER TEST CONDITIONS
- Photoperiod: day : night-rhythm 16 : 8 h
- Light intensity: about 5 -6 µE/(m²*s) in the range of 400-700 nm

EFFECT PARAMETERS MEASURED: swimming ability after 0, 3, 6, 24, 48 h

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

12.5 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

31.7 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: 95% CL: 27.4 -36-7 mg/L

Effect concentrations 3

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

50 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

- Mortality of control: none