Divinylbenzene

Endpoint summary

Description of key information

Short term toxicity to fish:

Short term acute toxicity of fish was evaluated for the test chemical 1,2-di(ethenyl)benzene on fish Oryzias latipes for 96 h in static condition .The median lethal effect concentration (LC50) for 50% mortality was observed to be 4.2 mg/l . On the basis of above concentration it can be considered that test material is toxic for fish . Hence, it can be classified as aquatic chronic – 2 as per CLP criteria.

 

Long term toxicity to fish:

Long term toxicity of target chemical 1,2-di(ethenyl)benzene was observed on fish for 14 days, The effect concentarions of the test chemical was reported as LC50 1.8 and the no effect concentration was noted to be 0.46 mg/l after 14 days of exposure.

On the basis of above effect concentration it can be concluded that the test chemical 1,2-di(ethenyl)benzene has long term effect on fish and can be classified as aquatic chronic - 3 on the basis NOEC value.

Short term toxicity to aquatic invertebrate:

Short term toxicity to aquatic invertebrate was evaluated for Daphnia magna for the test chemical 1,2-di(ethenyl)benzene for 48 h . The effect concentration (EC50) of test material 1,2-di(ethenyl)benzene on Daphnia magna after 48 h was observed to be 1.9 mg/l. On the basis of above effect concentration it was considered that the test chemical is toxic to aquatic invertebrate . Hence, it can be classified as aquatic chronic-2 as per CLP criteria.

Long term toxicity to aquatic invertebrate:

Long term toxicity of aquatic invertebrate was evaluated for aquatic invertebrtae for 21 days under static conditions. The effect concentration (EC50) and (NOEC) for long term toxicity of test material on aquatic invertebrate was observed to be 0.72 mg/l and 0.35 mg/l.On the basis of above effect concentration it can be considered that test chemical 1,2-di(ethenyl)benzene can be toxic to aquatic invertebrate in longg term and can be classified as aquatic chronic-2.

Toxicity to aquatic algae and cyanobacteria:

Toxicity to aquatic algae and cyanobacteria was evaluated for test chemical 1,2-di(ethenyl)benzene. The effect concentration for growth rate and area under growth curve were observed . The effect concentration (EC50) for the growth rate and area under growth curve was in the range of 1.7 to 1.8 mg/l. On the basis of above test concentration it can be concluded that the test chemical 1,2-di(ethenyl)benzene was toxic to aquatic algae and cyanobateria and can be classified as aquatic chronic 2 as per CLP criteria.

Toxicity to microorganism:

Data available for the closely related read across chemicals has been reviewed to determine the short term toxicity of microorganisms of the test chemical 1,2-di(ethenyl)benzene (1321 -74 -0).The studies are as mentioned below:

1.Vibrio parahaemolyticus was exposed to test material for 9 hrs at 30°C at complex medium using Ethanol as a solvent .

 Minimum inhibition concentration was calculated using arithmetic difference between percentage transmittance (620 nm) of control (ethanol only) and test culture. Minimum inhibition concentration of Decanoic acid on Vibrio parahaemolyticus was observed to be 60mg/l.

2.Cell multiplication inhibition test was carried out to study the effects of test material on Pseudomonas putida. The threshold toxcity of test material on cell multiplication inhibition effect on Pseudomonas putida was observed to be 62 mg/L.

Hence,based on the data available for the structurally and functionally similar read across, test chemical 1,2-di(ethenyl)benzene is not likely to be toxic to microorganisms atleast in the concentration range of 60- 62 mg/L .

Additional information

Short term toxicity to fish:

Data for target chemical 1,2-di(ethenyl)benzene along with its structurally similar read across substance was used to summarize the short term toxicity of test chemical:

1.Short term acute toxicity of fish was evaluated for the test chemical 1,2-di(ethenyl)benzene on fish Oryzias latipes for 96 h in static condition .The median lethal effect concentration (LC50) for 50% mortality was observed to be 4.2 mg/l . On the basis of above concentration it can be considered that test material is toxic for fish . Hence, it can be classified as aquatic chronic – 2 as per CLP criteria.

2.Short term acute toxicity of fish was evaluated for the test chemical 1,2-di(ethenyl)benzene on fish Oryzias latipes for 48 h in static condition . The median lethal effect concentration (LC50) for 50% mortality was observed to be 3.45 mg/l . On the basis of above concentration it can be considered that test material is toxic for fish . Hence, it can be classified as aquatic chronic – 2 as per CLP criteria.

3.Short term toxicity of test material was evaluate on fish Fathead minnow. A I0 mL aliquot of each sample was transferred to a purge vessel with a 50 mL gastight syringe. Samples outside the calibration range were diluted with deionized water. The test was carried out in sealed containers with little or no head space. LC50 were calculated using the probit method. The slope of the concentration-response curve was also calculated using the probit method.

The median lethal effect concentration (LC50) was observed to be 5.2 mg/l with 95% confidence interval = 4.6 to 5.9 mg/L. Hence , on the basis of the above effect concentration it can be considered that the test chemical is toxic to fish and can be classified in the category aquatic chronic -2 as per CLP criteria

 

 

Long term toxicity to fish:

Long term toxicity of target chemical 1,2-di(ethenyl)benzene was observed on fish for 14 days, The effect concentarions of the test chemical was reported as LC50 1.8 and the no effect concentration was noted to be 0.46 mg/l after 14 days of exposure.

On the basis of above effect concentration it can be concluded that the test chemical 1,2-di(ethenyl)benzene has long term effect on fish and can be classified as aquatic chronic - 3 on the basis NOEC value.

Short term toxicity to aquatic invertebrate:

Data for target chemical 1,2-di(ethenyl)benzene along with its structurally similar read across substance was used to summarize the short term toxicity of test chemical:

1.Short term toxicity to aquatic invertebrate was evaluated for Daphnia magna for the test chemical 1,2-di(ethenyl)benzene for 48 h . The effect concentration (EC50) of test material 1,2-di(ethenyl)benzene on Daphnia magna after 48 h was observed to be 1.9 mg/l. On the basis of above effect concentration it was considered that the test chemical is toxic to aquatic invertebrate . Hence, it can be classified as aquatic chronic-2 as per CLP criteria.

2.Short term toxicity of test material was evaluated for aqutaic invertebrate Daphnia magna according to OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test). The effect concentration (EC50) of test chemical on Daphnia magna was evaluted to be 2.6 mg/l aftter 48 h of test exposure.

On the basis of above effect concentration it can be concluded that the test material is toxic to aquatic invertebrate . Hence, the test material an be classified as aquatic chronic - 2

3.Short term toxicity to aquatic invertebrate was evaluated for the test material , Daphnia magna Straus neonates, less than 20 hours old was used for the test . A 100 mg/ml stock solution of the test material in reagent grade DMF was prepared by weight to a precision of 0.1 mg; additional stocks were prepared by serial dilution.The test concentration selected were 0.56, 1 .00, 1.80, 3.20, and 5.60 mg/l.

The concentration of test material lethal to 50% of the test population and 95% confidence limits were determined for the 24 and 48 hour exposure periods by the Spearman-Karber Estimator.

The 48-hour LC50 and 95% confidence limits in Daphnia magna were 3.34 (2.69-4.13) mg/L. The 48-hour NOEC was observed to be 0.56 mg/L.

On the basis of above effect concentration it can be concluded that the test material is toxic to aquatic invertebrate . Hence, the test material an be classified as aquatic chronic - 2.

Long term toxicity to aquatic invertebrate:

Long term toxicity of aquatic invertebrate was evaluated for aquatic invertebrtae for 21 days under static conditions. The effect concentration (EC50) and (NOEC) for long term toxicity of test material on aquatic invertebrate was observed to be 0.72 mg/l and 0.35 mg/l.On the basis of above effect concentration it can be considered that test chemical 1,2-di(ethenyl)benzene can be toxic to aquatic invertebrate in longg term and can be classified as aquatic chronic-2.

Toxicity to aquatic algae and cyanobacteria:

Data for target chemical 1,2-di(ethenyl)benzene along with its structurally similar read across substance was used to summarize the short term toxicity of test chemical:

1.Toxicity to aquatic algae and cyanobacteria was evaluated for test chemical 1,2-di(ethenyl)benzene. The effect concentration for growth rate and area under growth curve were observed . The effect concentration (EC50) for the growth rate and area under growth curve was in the range of 1.7 to 1.8 mg/l. On the basis of above test concentration it can be concluded that the test chemical 1,2-di(ethenyl)benzene was toxic to aquatic algae and cyanobateria and can be classified as aquatic chronic 2 as per CLP criteria.

2.Toxicity to aquatic algae and cyanobacteria was evaluated for aquatic algae and cyanobacteria . The effect concentration (EC50) for the growth rate was observed to be 2.6 mg/l . No observed effect concentration (NOEC) was observed to be 0.30 mg/l.On the basis of above effect concentration it can be considered that the test material was toxic to aquatic algae and cyanobacteria and can be classified as aquatic chronic-2.

3.Toxicity to algae (Selenastrum capricornutum, UTEX 1648) was evaluated according to OECD Method 201. The culture was transferred to sterile enriched media identical to media used for this test and maintained at test conditions for at least 14 days before the definitive test.

During the acclimation period, the culture was actively growing in at least 2 subcultures prior to the start of the toxicity test. The subsample of algae used to inoculate media at the start of the definitive test came from a seven day old culture. A series of solutions was prepared by bringing 0.0010, 0.0020, 0.0040, 0.0081, and 0.0160 g of test substance to 1,000 mL with dilution water in sealed glass bottles. The solutions were mixed on magnetic stirrers for approximately four hours.

The 72-hour EC50s were calculated using the weighted least squares non-linear regression estimation procedure The no observed effect concentration (NOEC) was determined using a one-way analysis of variance (ANOVA) and Bonferroni’s test . The effect concentration (EC50) 72 hours resulted in an EC50 of 2.6 mg/L the NOEC was observed at the concentration 1.6 mg/l.

On the basis of above effect concentration it can be considered that the test material was toxic to aquatic algae and cyanobacteria and can be classified as aquatic chronic-2.

Toxicity to microorganism:

Data available for the closely related read across chemicals has been reviewed to determine the short term toxicity of microorganisms of the test chemical 1,2-di(ethenyl)benzene (1321 -74 -0).The studies are as mentioned below:

1.Vibrio parahaemolyticus was exposed to test material for 9 hrs at 30°C at complex medium using Ethanol as a solvent .

 Minimum inhibition concentration was calculated using arithmetic difference between percentage transmittance (620 nm) of control (ethanol only) and test culture. Minimum inhibition concentration of Decanoic acid on Vibrio parahaemolyticus was observed to be 60mg/l.

2.Cell multiplication inhibition test was carried out to study the effects of test material on Pseudomonas putida. The threshold toxcity of test material on cell multiplication inhibition effect on Pseudomonas putida was observed to be 62 mg/L.

Hence,based on the data available for the structurally and functionally similar read across, test chemical 1,2-di(ethenyl)benzene is not likely to be toxic to microorganisms atleast in the concentration range of 60- 62 mg/L .