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EC number: 215-325-5 | CAS number: 1321-74-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Justification for type of information:
- Data is from authorative database
- Qualifier:
- according to guideline
- Guideline:
- other: as mentioned below
- Principles of method if other than guideline:
- To evaluate short term toxicity of aquatic algae and cyanobacteria for the test chemical 1,2-di(ethenyl)benzene
- GLP compliance:
- not specified
- Specific details on test material used for the study:
- -IUPAC name: 1,2-di(ethenyl)benzene-Mol. formula: C10H10-Molecular Weight: 131.1969 g/mol- Substance type: Organic- Physical state: Pale, straw-colored liquid
- Test organisms (species):
- other: Green algae
- Test type:
- static
- Water media type:
- freshwater
- Total exposure duration:
- 72 h
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 1.8 mg/L
- Nominal / measured:
- not specified
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.91 mg/L
- Nominal / measured:
- not specified
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 1.7 mg/L
- Nominal / measured:
- not specified
- Conc. based on:
- test mat.
- Basis for effect:
- other: area under growth curve
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.81 mg/L
- Nominal / measured:
- not specified
- Conc. based on:
- test mat.
- Basis for effect:
- other: area under growth curve
- Validity criteria fulfilled:
- not specified
- Conclusions:
- The effect cocncentration (EC50) of test material based on growth rate was observed to be 1.8 mg/l
- Executive summary:
Toxicity to aquatic algae and cyanobacteria was evaluated for test chemical 1,2-di(ethenyl)benzene. The effect concentration for growth rate and area under growth curve were observed . The effect concentration (EC50) for the growth rate and area under growth curve was in the range of 1.7 to 1.8 mg/l. On the basis of above test concentration it can be concluded that the test chemical 1,2-di(ethenyl)benzene was toxic to aquatic algae and cyanobateria and can be classified as aquatic chronic 2 as per CLP criteria.
Reference
Description of key information
Toxicity to aquatic algae and cyanobacteria:
Toxicity to aquatic algae and cyanobacteria was evaluated for test chemical 1,2-di(ethenyl)benzene. The effect concentration for growth rate and area under growth curve were observed . The effect concentration (EC50) for the growth rate and area under growth curve was in the range of 1.7 to 1.8 mg/l. On the basis of above test concentration it can be concluded that the test chemical 1,2-di(ethenyl)benzene was toxic to aquatic algae and cyanobateria and can be classified as aquatic chronic 2 as per CLP criteria.
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 1.7 mg/L
Additional information
Toxicity to aquatic algae and cyanobacteria:
Data for target chemical 1,2-di(ethenyl)benzene along with its structurally similar read across substance was used to summarize the short term toxicity of test chemical:
1.Toxicity to aquatic algae and cyanobacteria was evaluated for test chemical 1,2-di(ethenyl)benzene. The effect concentration for growth rate and area under growth curve were observed . The effect concentration (EC50) for the growth rate and area under growth curve was in the range of 1.7 to 1.8 mg/l. On the basis of above test concentration it can be concluded that the test chemical 1,2-di(ethenyl)benzene was toxic to aquatic algae and cyanobateria and can be classified as aquatic chronic 2 as per CLP criteria.
2.Toxicity to aquatic algae and cyanobacteria was evaluated for aquatic algae and cyanobacteria . The effect concentration (EC50) for the growth rate was observed to be 2.6 mg/l . No observed effect concentration (NOEC) was observed to be 0.30 mg/l.On the basis of above effect concentration it can be considered that the test material was toxic to aquatic algae and cyanobacteria and can be classified as aquatic chronic-2.
3.Toxicity to algae (Selenastrum capricornutum, UTEX 1648) was evaluated according to OECD Method 201. The culture was transferred to sterile enriched media identical to media used for this test and maintained at test conditions for at least 14 days before the definitive test.
During the acclimation period, the culture was actively growing in at least 2 subcultures prior to the start of the toxicity test. The subsample of algae used to inoculate media at the start of the definitive test came from a seven day old culture. A series of solutions was prepared by bringing 0.0010, 0.0020, 0.0040, 0.0081, and 0.0160 g of test substance to 1,000 mL with dilution water in sealed glass bottles. The solutions were mixed on magnetic stirrers for approximately four hours.
The 72-hour EC50s were calculated using the weighted least squares non-linear regression estimation procedure The no observed effect concentration (NOEC) was determined using a one-way analysis of variance (ANOVA) and Bonferroni’s test . The effect concentration (EC50) 72 hours resulted in an EC50 of 2.6 mg/L the NOEC was observed at the concentration 1.6 mg/l.
On the basis of above effect concentration it can be considered that the test material was toxic to aquatic algae and cyanobacteria and can be classified as aquatic chronic-2
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