Ethyl acetoacetate

Acute Toxicity: inhalation

Administrative data

Endpoint:

acute toxicity: inhalation

Type of information:

migrated information: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: see 'Remark'

Remarks:

Read-across from supporting substance: - Substance: Methyl acetate - CAS no.: 79-20-9 - EC no.: 201-185-2 Read-across justification: Ethyl acetoacetate is a well defined mono-constitutent substance of high purity. Based on the similar structure and properties, the substance Methyl acetate was selected as representative model structure for read-across. Compared to EAA, the selected structure show similar physical-chemical, toxicological and ecotoxicological properties. A data matrix covering all available endpoints was previously published in the EU-Risk assessment Report Vol. 34 of 2003. The available data on EAA and selected model constituent revealed similar properties. In general, the classification and labeling of the model constituent is comparable to that of Eethyl acetoacetate. Neither the model constituents nor MAA are considered PBT or vPvB substances. In summary, the read-across between MAA and the model constituent is justified. Result: Smyth et al screened industrial chemicals for acute toxicity values. For methylacetate the following values were determined: - LC0 = 49.2 mg/l/4h - LC50 = > 49.2 mg/l/4h

Data source

Reference

Reference Type:

publication

Title:

Unnamed

Year:

2003

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

Concentrated vapor inhalation consists of exposing 6 male or female albino rats to a following stream of vapor-loaded air. The vapor-air mixture is generated by passing 2.5L /minute of dried air at room temperature through a fritted glass disc immersed to a depth of at least one inch in approximately 50 ml of the test chemical contained in a gas-washing bottle. Inhalations are continued for time periods in a logarithmic series with a ratio of two extending from one-fourth to eight hours, until the inhalation period killing about half the number of rats within 14 days is defined. Inhalation of metered vapor concentrations by rats is conducted with flowing streams of vapor prepared by various styles of proportioning pumps. Inhalation periods are usually four hours' duration unless slight toxicity enforces an eight hour period. Concentration recorded are nominal and not analytically verified. They are in an essentially logarithmic series with a factor of two, and the Table records the concentration yielding fractional mortality among 6 rats within 14 days. Where no fractional mortality was observed, usually both the concentration yielding no mortality and that yielding complete mortality are indicated.

GLP compliance:

no

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Details on test material:

- Name of the test substamce: Methyl acetate
- CAS No: 79-20-9
- E No: 201-185-2

Test animals

Species:

rabbit

Strain:

other: Albino

Sex:

male/female

Administration / exposure

Route of administration:

inhalation: vapour

Type of inhalation exposure:

whole body

Vehicle:

other: unchanged (no vehicle)

Analytical verification of test atmosphere concentrations:

no

Duration of exposure:

4 h

Concentrations:

16,000 ppm and 32,000 ppm

No. of animals per sex per dose:

6 animals

Control animals:

no

Results and discussion

Effect levels

Effect levels 1

Dose descriptor:

LC0

Effect level:

49.2 mg/L air

Exp. duration:

4 h

Effect levels 2

Dose descriptor:

LC50

Effect level:

> 49.2 mg/L air

Exp. duration:

4 h

Any other information on results incl. tables

49.2 mg/l < LC50< 98.4 mg/l

Applicant's summary and conclusion

Interpretation of results:

not classified

Remarks:

Migrated information Criteria used for interpretation of results: EU

Conclusions:

Read-across to Methyl acetate:
The acute inhalative LC50 (4hr) of Methyl acetate on Albino rabbits was determined to be > 49.2 mg/L air.

Executive summary:

Read-across from supporting substance:

- Substance: Methyl acetate

- CAS no.: 79 -20 -9

- EC no.: 201 -185 -2

Smyth et al screened industrial chemicals for acute toxicity values. For methylacetate the following values were determined:

- LC0 = 49.2 mg/l/4h

- LC50 = > 49.2 mg/l/4h