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Environmental fate & pathways

Biodegradation in water: screening tests

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Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
weight of evidence
Study period:
17.06. to 15.07.1998
Reliability:
1 (reliable without restriction)
Qualifier:
according to guideline
Guideline:
OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I))
Deviations:
no
GLP compliance:
yes
Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, non-adapted
Details on inoculum:
From 10 areas: Fushiko River disposal site (Sapporo, Hokkaido), Kashima disposal site (Kashima, Ibaragi), Nakahama disposal site (Osaka), Ochiai disposal site (Shinjyuku, Tokyo), Kitagami River (Ishimaki, Miyagi), Shinano River (Nishi Kanbara, Niigata), Yoshino river (Tokushima), Lake Biwa (Otsu, Shiga), Hiroshima Bay (Hiroshima), Doukai Bay (Kita Kyusyu, Fukuoka)
Collected in March 1998
How to collect (1) sewage: returned sludge at disposal site (2) river, lake, sea: top soil at the water’s edge, which has exposure to air and surface water
mixture of new and old sludge: mixed filtrate 5 L of mixed sludge liquid collected from above mentioned areas and filtrate 5 L of active sludge (2), which was cultured 3 months, and then adjusted pH 7.0+-1.0 and aerated (3) in a fermenter
(2) active sludge, which is 10L mixed sludge liquid corrected from above mentioned areas cultured according to 11.4.
(3) out door air through pre-filter was used for aeration.
Cultivation: after aeration in the fermenter was stopped for around 30 min., 1/3 of clear supernatant liquid was removed. Then, added same volume of dechlorinated water, aerated again, and added synthesized sewage (4) to have concentration of clear supernatant liquid at 0.1%. Repeated this operation once a day to cultivate and to reach active sludge. Temperaturen for cultivation was set at 25℃ +- 2℃
(4) dissolved glucose, peptone, calcium dihydrogen phosphate into the synthesized sewage, so that each reaches 4 (W/V) % and adjusted pH 7.1+-1.0 by using sodium hydroxide

Duration of test (contact time):
28 d
Initial conc.:
100 mg/L
Based on:
test mat.
Parameter followed for biodegradation estimation:
O2 consumption
Details on study design:
3 vessels with sludge and test substance: 100 mg/l test substance; 30 mg/l activated sludge, the Ph value was adjusted from ca 10 to ca 7 with sodium hydroxide
1 vessel with sludge and reference substance: 100 mg/l aniline; 30 mg/l activated sludge
1 vessel control blank
1 vessel water plus test substance
Tempereature: 25°C+-1°C
Reference substance:
aniline
Preliminary study:
no
Test performance:
regular test under regular conditions
Key result
Parameter:
% degradation (O2 consumption)
Remarks:
BOD
Value:
88
Sampling time:
28 d
Remarks on result:
other: readily biodegradable
Key result
Parameter:
% degradation (test mat. analysis)
Remarks:
HPLC
Value:
100
Sampling time:
28 d
Remarks on result:
other: readily biodegradable
Key result
Parameter:
% degradation (TOC removal)
Value:
96
Sampling time:
28 d
Remarks on result:
other: readily biodegradable
Results with reference substance:
BOD = 72.1 mg after 28 days
Validity criteria fulfilled:
yes
Interpretation of results:
readily biodegradable
Executive summary:

The test was carried out according to OECD 301 C (MITI) guideline under GLP conditions. The test results showed a biodegradation of 88% under the test conditions and the substance can be considered as readily biodegradable.

Endpoint:
biodegradation in water: ready biodegradability
Data waiving:
study technically not feasible
Justification for data waiving:
other:
Justification for type of information:
Titanium tetrakic(dimethylammonium) can only be handled air-free. It is a flammable liquid and very unstable (detailed description in chapter 1.2 or 5.2.1 or 13). Within seconds it reacts violently in contact with moisture or water. A biodegradation test in water is technically not feasible. The decomposition products are dimethylamine and titanium dioxide. Available data of dimethylamine show, this substance is readily biodegradable. Titanium dioxide is an inorganic substance and a biodegradation test is technically not feasible.
Dimethylamine is found quite widely distributed in animals and plants, and is present in many foods at the level of a few mg/kg.

Description of key information

Titanium tetrakic(dimethylammonium) can only be handled air-free. It is a flammable liquid and very unstable (detailed description in chapter 1.2 or 5.2.1 or 13). Within seconds it reacts violently in contact with moisture or water. A biodegradation test in water is technically not feasible. The decomposition products are dimethylamine and titanium dioxide. Available data of dimethylamine show, this substance is readily biodegradable. Titanium dioxide is an inorganic substance and a biodegradation test is technically not feasible.

Dimethylamine is found quite widely distributed in animals and plants, and is present in many foods at the level of a few mg/kg.

Key value for chemical safety assessment

Additional information