Titanium tetrakis(dimethylammonium)

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

not specified

Specific details on test material used for the study:

a 40% solution of dimethylamine in water was used for the test
supplied by Fluka, 99% purity

Analytical monitoring:

not specified

Vehicle:

not specified

Details on test solutions:

the stock solutions were prepared fresh each day. The test solutions were renewed daily

Test organisms (species):

Daphnia magna

Details on test organisms:

daphnieds were feed with Chlorella pyrenoidosa

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Post exposure observation period:

no

Hardness:

as CaCO3 = 260

Test temperature:

20+-0.5°C

pH:

8.1+-0.2

Dissolved oxygen:

not specified

Nominal and measured concentrations:

nominal: 10, 18, 32, 56, 100, 180 mg/l
control

Reference substance (positive control):

not specified

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

50 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

95%C.L. = 42.2 - 56.5

Reported statistics and error estimates:

The EC50 values and their 95% confidence intervals were calculated according to Lichtfield and Wilkoxon (1949) method

Validity criteria fulfilled:

not specified

Executive summary:

An EC50 value of 50 mg/l were determined in an acute 48h daphnia test according to OECD guideline 202

Reference 2

Endpoint:

short-term toxicity to aquatic invertebrates

Data waiving:

study technically not feasible

Justification for data waiving:

other:

Justification for type of information:

Titanium tetrakic(dimethylammonium) is an organometalic compound. It can only be handled air-free. It is a flammable liquid and very unstable (see also chapter 1.2 or 5.1.2 or 13). Within seconds it reacts violently in contact with moisture or water. Aquatic toxicity tests are technically not feasible. The decomposition products are dimethylamine and titanium oxide. Dimethylamine is a highly flammable gas. But dimethylamine is a well water soluble substance and was tested for acute aquatic toxicity.

Reference 3

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Qualifier:

according to guideline

Guideline:

other: AFNOR Test

Version / remarks:

Norme Experimentale N.F.T. 90-301 (1974)

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Version / remarks:

version: 4 April, 1984
Part I: the 24h EC50 acute immobilisation test (daphnia sp.)

Principles of method if other than guideline:

to define the 24h IC50 for daphnia (immobilization concentration for 50% of the animals at 24h)

GLP compliance:

not specified

Specific details on test material used for the study:

dimethylamine as 40% aqueous solution

Analytical monitoring:

yes

Details on sampling:

at the beginning of the test
frequently checking during the test
at the end of the test

Vehicle:

no

Test organisms (species):

Daphnia magna

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

24 h

Remarks on exposure duration:

the exposure duration was in conformance to the prior guidelines

Post exposure observation period:

no

Hardness:

no information

Test temperature:

no information

pH:

no information

Dissolved oxygen:

no information

Nominal and measured concentrations:

no information

Reference substance (positive control):

not specified

Duration:

24 h

Dose descriptor:

IC50

Effect conc.:

48 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

An IC50 of 48 mg/l was determined with CL (95%) of 44-52 and S = 1.13 (slope of the efect concentration curves)

Results with reference substance (positive control):

no information

Reported statistics and error estimates:

IC50 determination according to Litchfield and Wilcoxon 1949

Validity criteria fulfilled:

not specified

Executive summary:

For dimethylamine an IC50 of 48 mg/l was dertermined in an 24 h acute immobilisation test carried out according to AFNOR protocol

Description of key information

Titanium tetrakic(dimethylammonium) is an organometalic compound. It can only be handled air-free. It is a flammable liquid and very unstable (see also chapter 1.2 or 5.1.2 or 13). Within seconds it reacts violently in contact with moisture or water. Aquatic toxicity studies ate technically not feasible. The decomposition products are dimethylamine and titanium oxide. Dimethylamine is a highly flammable gas. But dimethylamine is a well water soluble substance and was tested for acute aquatic toxicity. For dimethylamine an EC50 value of 50 mg/l were determined in an acute 48h daphnia test according to OECD guideline 202 (Van Leeuwen 1985). In a second test (Calamari et al. 1980) an IC50 of 48 mg/l was dertermined for dimethylmine in an 24 h acute immobilisation test carried out according to AFNOR protocol