7-azatridecane-1,13-diamine

Specific investigations: other studies

Link to relevant study record(s)

Reference

Endpoint:

endocrine system modulation

Type of information:

experimental study

Adequacy of study:

supporting study

Study period:

year of publication: 2009

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Qualifier:

equivalent or similar to guideline

Guideline:

other: Estrogen Receptor Binding Assay Using Rat Uterine Cytosol (OSCPP 890.1250)

Version / remarks:

guideline was not implemented at the time of experiment, however experiment contributed to guideline setup

GLP compliance:

not specified

Type of method:

in vitro

Endpoint addressed:

other: estrogen receptor binding

Species:

rat

Strain:

Sprague-Dawley

Remarks:

estrogen receptors from Rat Uterine Cytosol (RUC) preparations

Sex:

female

Details on results:

The submission substance was part of EPAs Safer Chemicals Research, including the ToxCast and Tox21 high-throughput toxicology efforts. As part of these initiatives the submission substance was one of many chemicals tested in a Estrogen Receptor Binding Assay Using Rat Uterine Cytosol (ER-RUC).
The result reported for the submission subtance is 'inactive', this either means that 'no binding was observed when tested to the concentration limit of 100uM, or some binding was observed in initial screening but secondary analysis confirmed non-binder status'.

Conclusions:

The submission substance was inactive in the Estrogen Receptor Binding Assay Using Rat Uterine Cytosol (ER-RUC; i.e. no binding to the estrogen receptor will occur).

Description of key information

The submission substance was inactive in the Estrogen Receptor Binding Assay Using Rat Uterine Cytosol (ER-RUC; i.e. no binding to the estrogen receptor will occur).

Additional information

The submission substance was part of EPAs Safer Chemicals Research, including the ToxCast and Tox21 high-throughput toxicology efforts. As part of these initiatives the submission substance was

one of many chemicals tested in an Estrogen Receptor Binding Assay Using Rat Uterine Cytosol (ER-RUC).

Principles of the test:

In the experimental study, the relative binding affinities (RBAs) were determined by the ability of the natural ligand, to bind to the estrogen receptors in a rat tissue preparation in the presence of increasing amounts of the test chemical. It simulates the initiating step of a process that results in estrogen receptor mediated physiological responses. Chemicals that are active in this assay may have tissue dependent agonist and/or antagonist properties. Measuring the response as a function of the concentration of the test chemical allows true binders to be separated from chemicals that interfere with the binding of the natural ligand through other mechanisms.