Glycine max. (L.) Merr. (Fabacee) aqueous alcoholic ext., concentrate by sequential extractions and filtration

Surface tension

Administrative data

Endpoint:

surface tension

Type of information:

experimental study

Adequacy of study:

key study

Study period:

May 06 - 07, 2013

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2013

Report date:

2013

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 115 (Surface Tension of Aqueous Solutions)

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method A.5 (Surface Tension)

GLP compliance:

no

Type of method:

OECD harmonised ring method

Test material

Test material information

Constituent 1

Reference substance name:

Glycine max. (L.) Merr. (Fabacee) aqueous alcoholic ext., concentrate by sequential extractions and filtration

EC Number:

938-980-5

IUPAC Name:

Glycine max. (L.) Merr. (Fabacee) aqueous alcoholic ext., concentrate by sequential extractions and filtration

Test material form:

solid: particulate/powder

Remarks:

migrated information: powder

Details on test material:

Test item identification : Soybean (Glycine max) dry purified extract
Active ingredient content : 100 % w/w (from the Test Substance Data Sheet (TSDS)
Batch number : 30432/M1

Results and discussion

Surface tension

Surface tension:

33.7 mN/m

Temp.:

20 °C

Remarks on result:

other: aqueous solution at 90% of its saturation

Any other information on results incl. tables

The determinations were performed at 20.0 ± 0.5°C and at this temperature the tabulate value for water is 72.75 mN/m. After zero point adjustment, the surface tension of the distilled water (σg) was determined to be 72.45 mN/m; therefore the calibration factor was calculated to be f (water) = 1.0041. According to the method’s indications, the test item was tested at 90% of the saturation solubility in water. The test item solutions at the above described concentration was maintained at 20 ± 0.5°C in a thermostatic liquid bath and then tested.

Soybean (Glycine max) dry purified extract: surface tension at 20°C

Measurements	Test item 90% saturation (mN/m)
1st	35.1
2nd	35.1
3rd	35.5
4th	35.5
5th	35.5
6th	35.7
Average values (a)	35.4
S.D.	0.24
RSD %	0.69%

 

Soybean (Glycine max) dry purified extract: surface tension at 20°C. Correction with water calibration factor

	Average values (a) (mN/m)	f (water) calibration factor	Corrected values (b) (mN/m)
Test item 90% saturation	35.4	1.0041	35.6

 

Soybean (Glycine max) dry purified extract: surface tension at 20°C. Correction with method interpolation

 

Test item solution (mg/L)	Corrected values (b) (mN/m)	Tab. X1 (mN/m)	Tab. X2 (mN/m)	Tab. Y1 (mN/m)	Tab. Y2 (mN/m)	Slope (m)	Interc. (q)	St (mN/m)
Test item 90% saturation	35.6	32.1	34.1	34.0	36.0	1.0000	1.9000	33.7

Note.

Y1 and Y2 are the surface tension values respectively lower and higher of the experimental datum obtained after first correction with water calibration. X1 and X2 are the correspondent values found in the table reported in the A.5 method. Using these values a linear interpolation was calculated through the following formula: Y = m X + q where: m = Slope of the linear interpolation (from the table) q = Interception value (mN/m) of the linear interpolation (from the table)

Applicant's summary and conclusion

Conclusions:

From the experimental data obtained according to the guideline A.5 in Council Regulation (EC) No 440/2008 part A and OECD Guideline No. 115, it can be concluded that the surface tension at 20°C is 33.7 mN/m for the Soybean (Glycine max) dry purified extract sample in an aqueous solution at 90% of its saturation.
The substance should be regarded as a surface-active material.