Chlorosulphonyl isocyanate

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Remarks:

Type of genotoxicity: gene mutation

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Report date:

1987

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

EU Method B.13/14 (Mutagenicity - Reverse Mutation Test Using Bacteria)

Test guideline 2

Qualifier:

according to guideline

Guideline:

OECD Guideline 471 (Bacterial Reverse Mutation Assay)

GLP compliance:

yes

Type of assay:

bacterial reverse mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

Chlorosulphonyl isocyanate

EC Number:

214-715-2

EC Name:

Chlorosulphonyl isocyanate

Cas Number:

1189-71-5

Molecular formula:

CClNO3S

IUPAC Name:

[(chlorosulfonyl)imino]methanone

Test material form:

gas under pressure: refrigerated liquefied gas

Details on test material:

- Name of test material: Chlorosulfonyl isocyanate

Method

Target gene:

Base pair exchange type and frame shift mutations.

Species / strain

Species / strain / cell type:

S. typhimurium TA 1535, TA 1537, TA 98, TA 100 and E. coli WP2

Additional strain / cell type characteristics:

not applicable

Metabolic activation:

with and without

Metabolic activation system:

Activated rat liver microsomal S-9 fraction

Test concentrations with justification for top dose:

5000, 2500, 1250, 625, 313 and 157 µg/plate

Controls

Untreated negative controls:

yes

Negative solvent / vehicle controls:

yes

True negative controls:

no

Positive controls:

yes

Positive control substance:

other: NaN3, ICR 191, AF-2, 2AA

Results and discussion

Test results

Species / strain:

S. typhimurium TA 1535, TA 1537, TA 98, TA 100 and E. coli WP2

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

cytotoxicity

Vehicle controls validity:

valid

Untreated negative controls validity:

not examined

Positive controls validity:

valid

Remarks on result:

other: all strains/cell types tested

Remarks:

Migrated from field 'Test system'.

Any other information on results incl. tables

No significant increase (two fold and dose-related increase compared with negative control) in revertant colonies per plate was observed at any concentration of Chlorosulfonyl isocyanate in the absence and presenceofS9 mix. Positive controls were tested in this study; NaN3, ICR-191, AF-2 and 2AA. These four positive controls showed significant increase of revertant colonies. It was concluded that Chlorosulfonyl isocyanate was not mutagenic in this test system.

Applicant's summary and conclusion

Conclusions:

Interpretation of results (migrated information):
negative

Chlorosulfonyl isocyanate was not mutagenic in this test system.

Executive summary:

A study was carried out according to EU Method B.13/14 and OECD Guideline 471 (Bacterial Reverse Mutation Assay). The mutagenic potential of the test item was assayed with S. typhimurium TA98, TA100, TA1535, and TA1537 and E. coli WP2 uvrA using the preincubation method in the absence and presence of S9 mix. The test item was tested at the dose of 157, 313, 625, 1250, 2500 and 5000 µg/plate. No significant increase in revertant colonies was observed at any concentration of test item, either in the absence and presence of S9 mix. Positive controls showed significant increase of the incidence of revertant colonies. It was concluded that the test item showed no mutagenic activity when tested in this bacterial test system.