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EC number: 268-083-8 | CAS number: 68002-70-0 This substance is identified by SDA Substance Name: C16-C22 trialkyl glyceride and SDA Reporting Number: 21-001-00.
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Study period:
- Not reported
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: The study was conducted according to ISO 10253 guideline. GLP compliance not reported.
Cross-referenceopen allclose all
- Reason / purpose for cross-reference:
- reference to same study
- Reason / purpose for cross-reference:
- reference to other study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 004
- Report date:
- 2004
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- ISO 10253 (Water quality - Marine Algal Growth Inhibition Test with Skeletonema costatum and Phaeodactylum tricornutum)
- Deviations:
- no
- Principles of method if other than guideline:
- Not applicable
- GLP compliance:
- not specified
Test material
- Reference substance name:
- Glycerides, C8-18 and C18-unsatd.
- IUPAC Name:
- Glycerides, C8-18 and C18-unsatd.
- Reference substance name:
- Glycerides, C8-18 and C18-unsatd.
- EC Number:
- 266-946-3
- EC Name:
- Glycerides, C8-18 and C18-unsatd.
- IUPAC Name:
- 266-946-3
- Reference substance name:
- 67701-28-4
- Cas Number:
- 67701-28-4
- IUPAC Name:
- 67701-28-4
- Details on test material:
- - Name of test material (as cited in study report): Palm kernel olein (CAS N° 91079-13-9, EC N° 293-399-8); under the SDA nomenclature, the name of this substance is ‘Glycerides, C8-18 and C18-unsatd.’
- Physical state: Dark yellow semi-solid
- Expiration date of the lot/batch: Apr. 28, 2004
- Storage condition of test material: Stored in the dark at room temperature
Constituent 1
Constituent 2
Constituent 3
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
Not applicable
Sampling and analysis
- Analytical monitoring:
- not specified
- Details on sampling:
- Not applicable
Test solutions
- Vehicle:
- yes
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Water accommodated fraction (WAF) methodology
- Preparation: WAFs were prepared by adding test material to the test medium (sterile marine algal media) to give loading rates of 10, 100 and 1,000 mg/L. In order to mix the test material, which was solid at room temperature, the sample was microwaved on full power for approximately 2 minutes until it became liquid. The contents of the WAF vessels were then stirred using a magnetic stirrer, at 300 rpm, for 24 h, in a controlled environment room set at 20 ± 2 °C and then left to settle for a minimum of 2 h (range 2 – 4 h), to allow the undissolved material to separate out. The aqueous phases - the WAFs – were then drawn off via the tap for use in the tests. Control media was sterile marine algal media.
Test organisms
- Test organisms (species):
- Skeletonema costatum
- Details on test organisms:
- TEST ORGANISM
- Source (laboratory, culture collection): Scottish Association for Marine Science Research Services Ltd, Dunstaffnage, Oban, Scotland
- Method of cultivation: An appropriate amount of culture was used to inoculate starter culture flasks containing sterile media. The flasks were incubated at 20 ± 2°C in a Gallenkamp orbital incubator under full constant illumination (9000 lux) for 4 days. This starter culture was used for inoculation of test cultures.
Study design
- Test type:
- static
- Water media type:
- saltwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Post exposure observation period:
- No data
Test conditions
- Hardness:
- No data
- Test temperature:
- 20 ± 2 °C
- pH:
- Control: 8.4 at the start to 9.5 after 72 h
Test: 8.2 - 8.4 at the start to 8.0 - 9.6 after 72 h - Dissolved oxygen:
- No data
- Salinity:
- No data
- Nominal and measured concentrations:
- Nominal concentration: 10, 100 and 1,000 mg/L
1,000 mg/L was equivalent to 5.1 mg total carbon/L - Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): Erlenmeyer flasks closed with ground glass stoppers
- Material, size, headspace, fill volume: 250 mL flasks filled with 100 mL test solution
- Initial cells density: 10,000 cells/mL
- No. of vessels per concentration (replicates): Three
- No. of vessels per control (replicates): Six
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Natural seawater, collected off the beach at Kinmel Bay to the west of Rhyl, Flintshire, N. Wales
- Total organic carbon of medium: 31.0 mg/L
OTHER TEST CONDITIONS
- Sterile test conditions: yes
- Light intensity and quality: 7,400-8,550 lux
- Temperature: 20 ± 2 °C
- Other: Flasks incubated in a cooled, orbital incubator (100 cycles/min)
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: Particle counting was performed on the contents of each flask at the start of the test and after 24, 48 and 72 h of incubation using electronic particle counter (Coulter Model ZM)
- Other: At the end of the test, estimations of the mean chain length of S. costatum for one flask from each test concentration were made by microscopic examination of at least 50 chains in a 1 mL sub sample. - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol; 1.5 mg/L
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 72 h
- Dose descriptor:
- EL50
- Effect conc.:
- 10 - 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- EL50
- Effect conc.:
- 100 - 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Details on results:
- - Exponential growth in the control (for algal test): yes
- Test material was completely toxic to S. costatum at a loading rate of 1,000 mg/L and showed some toxicity at 100 mg/L, where algal growth was initially inhibited but recovered after 48 hours (see Table 1 for details). - Results with reference substance (positive control):
- - Results with reference substance: Valid
- The area under the growth curve was reduced by 87% and the average specific growth rate was reduced by 37% compared to controls - Reported statistics and error estimates:
- None
Any other information on results incl. tables
Table 1: Growth of Skeletonema costatum exposed to WAFs of palm kernel olein and 1.5 mg/L solution of reference compound 3,5-DCP
Treatment |
Mean reduction in area under the growth curve (A) relative to unfiltered controls (%) |
Mean reduction in average specific growth rate (µ) relative to unfiltered controls (%) |
Mean number cells per chain |
||||
|
0-24 h |
0-48 h |
0-72 h |
0-24 h |
0-48 h |
0-72 h |
72 h |
Control |
- |
- |
- |
- |
- |
- |
4.3 |
Palm kernel olein 10 mg/L |
26 |
27 |
17 |
20 |
13 |
4.2 |
4.4 |
Palm kernel olein 100 mg/L |
87 |
97 |
93 |
76 |
>100 |
49 |
4.7 |
Palm kernel olein 1000 mg/L |
87 |
98 |
100 |
76 |
>100 |
>100 |
- |
3,5 -DCP (1.5 mg/L) | 79 | 94 | 87 | 66 | 90 | 3.7 | 4.1 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- not specified
- Remarks:
- No data on coefficient of variation
- Conclusions:
- Based on the results, the 72 h EbL50 and ErL50 of the test material were determined to be 10 - 100 and 100 - 1,000 mg/L, respectively.
- Executive summary:
A study was conducted to evaluate the acute toxicity of the palm kernel olein to Skeletonema costatum under static conditions. The procedures followed ISO 10253 (Water quality - Marine Algal Growth Inhibition Test with Skeletonema costatum and Phaeodactylum tricornutum).
Skeletonema costatum were exposed to water accommodated fractions prepared from the test material at concentrations ranging from 10 - 1,000 mg/L for 72 h and the range of loading rates within which a 50% reduction in algal growth occurred after 72 h was determined on the basis of both the area under the growth curve (EbL50) and specific growth rate (ErL50).
Based on the results, the 72 h EbL50 and ErL50 of the test material were determined to be 10 - 100 and 100 - 1,000 mg/L, respectively.
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