4,4'-[isopropylidenebis(4,1-phenyleneoxy)]dianiline

Bioaccumulation: aquatic / sediment

Administrative data

Endpoint:

bioaccumulation in aquatic species: fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

03 Sep - 28 Nov 1985

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1985

Report date:

1985

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: “Bioconcentration test of chemical substances in fish and shellfish” specified in the Test Method Relating to New Chemical Substances (Kanpogyo No.5, Yakuhatsu No.615, 49 Kikyoku No.392

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

4,4'-[isopropylidenebis(4,1-phenyleneoxy)]dianiline

EC Number:

235-985-8

EC Name:

4,4'-[isopropylidenebis(4,1-phenyleneoxy)]dianiline

Cas Number:

13080-86-9

Molecular formula:

C27H26N2O2

IUPAC Name:

4,4'-[isopropylidenebis(4,1-phenyleneoxy)]dianiline

Radiolabelling:

no

Sampling and analysis

Details on sampling:

- Sampling intervals/frequency for test organisms: 3 fish were tested from the 1st and 2nd concentration groups, 2 fish were used for each analysis
- Sample storage conditions before analysis: 1 fish with aluminum foil and kept frozen in the plastic bag
- Details on sampling and analysis of test organisms and test media samples: See detailed analytical method in section "Any other information on materials and methods incl tables".

Test solutions

Vehicle:

yes

Remarks:

dimethyl sulfoxide

Details on preparation of test solutions, spiked fish food or sediment:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: 1.00 g of the test substance and 20.0 g of the dispersant (hydrogenated castor oil HCO-40) were dissolved in dimethyl sulfoxide to obtain the constant volume of 100 mL. This solution was diluted with water to obtain the constant volume of 5.0 L to prepare 200 ppm (W/V) of stock solution.
- Controls: yes

Test organisms

Test organisms (species):

Oryzias latipes

Details on test organisms:

TEST ORGANISM
- Common name: Carp
- Strain: Oryzias latipes
- Length at study initiation (length definition, mean, range and SD): 10.3 ± 0.2 cm (average)
- Weight at study initiation (mean and range, SD): 0.29 g (average); 24.5 ± 2.7 g
- Lipid content at test initiation (mean and range, SD): 3.8 ± 0.4%

ACCLIMATION
- Acclimation period: 25°C for 20 days

Quarantine:
- Quarantine conditions: Fish were treated with a medicated bath of Terramycin solution (100 ppm)
- Quarantine duration: 24 hours under static conditions (2 times).

Study design

Route of exposure:

aqueous

Test type:

flow-through

Water / sediment media type:

natural water: freshwater

Total exposure / uptake duration:

8 wk

Test conditions

Test temperature:

25.0 ± 1.0 °C (24.5±1.0°C)

Details on test conditions:

TEST SYSTEM
Test vessel:
- Type: open
- Material, size, headspace, fill volume: Glass aquaria, volume 100 L
- Type of flow-through: Water flow 300 mL/min (432 L/day)

Nominal and measured concentrations:

1st concentration group: 0.5 ppm
2nd concentration group: 0.05 ppm

Reference substance (positive control):

not specified

Results and discussion

Lipid content

Remarks on result:

other:

Remarks:

no information were given in the report

Bioaccumulation factor

Bioaccumulation factor 1

Conc. / dose:

0.5 other: ppm

Type:

BCF

Value:

> 303 - < 327 dimensionless

Time of plateau:

56 d

Remarks on result:

other: 56 days after the test

Remarks:

a mean of 315

Bioaccumulation factor 2

Conc. / dose:

0.05 other: ppm

Type:

BCF

Value:

> 492 - < 518 dimensionless

Time of plateau:

56 d

Remarks on result:

other: 56 days after the test

Remarks:

a mean of 505

Depuration

Remarks on result:

other: no information were given in the report

Rate constants

Remarks on result:

other:

Remarks:

no information were given in the report

Any other information on results incl. tables

Test results

Number of days after the test	1st concentration group		2nd concentration group
	A B	BCF (bioconcentration factor)	A’ B’	BCF (bioconcentration factor)
3	A	98	A’	258
	B	96	B’	200
7	A	237	A’	390
	B	163	B’	548
14	A	331	A’	550
	B	272	B’	612
21	A	350	A’	458
	B	322	B’	487
28	A	323	A’	462
	B	299	B’	542
42	A	293	A’	580
	B	269	B’	504
56	A	303	A’	518
	B	327	B’	492

(Note 1) A, B in the 1st concentration group and A' and B' in the 2nd concentration group are the marks attached to the sample fish at the time of measurement of accumulation.

Applicant's summary and conclusion

Conclusions:

The BCF was determined to be 505 (mean of 492 and 518) after 56 days at 0.05 ppb.