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EC number: 235-985-8 | CAS number: 13080-86-9
- Life Cycle description
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- Endpoint summary
- Appearance / physical state / colour
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- Density
- Particle size distribution (Granulometry)
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- Solubility in organic solvents / fat solubility
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- Flash point
- Auto flammability
- Flammability
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- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
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- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
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- Nanomaterial specific surface area
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- Endpoint summary
- Stability
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- Environmental data
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- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
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- Additional toxicological data

Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 10 - 29 Sep 1983
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
- Remarks:
- no information on historical data, acceptance and evaluation criteria; only 1 assay performed, tested up to 8000 µg/plate
Data source
Reference
- Reference Type:
- other: Summary of translation of japanese report
- Title:
- Unnamed
- Year:
- 1 983
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Version / remarks:
- adopted in 1997
- GLP compliance:
- no
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- 4,4'-[isopropylidenebis(4,1-phenyleneoxy)]dianiline
- EC Number:
- 235-985-8
- EC Name:
- 4,4'-[isopropylidenebis(4,1-phenyleneoxy)]dianiline
- Cas Number:
- 13080-86-9
- Molecular formula:
- C27H26N2O2
- IUPAC Name:
- 4,4'-[isopropylidenebis(4,1-phenyleneoxy)]dianiline
Constituent 1
Method
- Target gene:
- his operon, trp operon
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Species / strain / cell type:
- S. typhimurium TA 1538
- Species / strain / cell type:
- E. coli WP2 uvr A
- Metabolic activation:
- with and without
- Metabolic activation system:
- cofactor-supplemented post-mitochondrial fraction (S9 mix), prepared from the livers of male rats, induced with PCB (KC-500)
- Test concentrations with justification for top dose:
- Following concentrations were used in main experiment (preincubation):
all strains: 1, 5, 10, 50, 100, 500, 1000, 5000, 8000 μg/plate (with and without metabolic activation) - Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: DMSO
Controls
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- Remarks:
- DMSO
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- 4-nitroquinoline-N-oxide
- 9-aminoacridine
- benzo(a)pyrene
- other: AF-2; ethyl-N-nitro-N'- nitrosoguanidine (ENNG), 2-aminoanthracene (2AA)
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: preincubation
DURATION
- Preincubation period: 20 min
- Exposure duration: 48 h
NUMBER OF REPLICATIONS: duplicate
DETERMINATION OF CYTOTOXICITY
- Method: inspection of bacterial background lawn
Results and discussion
Test results
- Species / strain:
- other: S. typhimurium TA 98, TA 100, TA 1535, TA 1537, TA 1538, E. coli WP2 uvr A
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity, but tested up to precipitating concentrations
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- valid
- Additional information on results:
- TEST-SPECIFIC CONFOUNDING FACTORS
- Precipitation: Precipitation of the test substance was observed at a concentration of 1000 µg/plate.
Any other information on results incl. tables
Table 1: Test results (experiment 1, preincubation)
With or without S9 Mix |
Test substance concentration (μg/plate) |
Number of revertant colonies per plate |
|||||||||||
Frameshift type |
Base-pair substitution type |
||||||||||||
TA 98 |
TA 1537 |
TA 1538 |
TA 100 |
TA 1535 |
WP2 uvr A (pKM 101) |
||||||||
- |
Solvent control (DMSO) |
25 |
26 |
12 |
16 |
30 |
32 |
139 |
142 |
34 |
37 |
34 |
35 |
27 |
20 |
34 |
145 |
40 |
35 |
||||||||
1 |
24 |
27 |
19 |
21 |
30 |
30 |
107 |
127 |
33 |
34 |
25 |
25 |
|
29 |
22 |
30 |
147 |
34 |
24 |
||||||||
5 |
39 |
31 |
12 |
12 |
23 |
31 |
144 |
147 |
37 |
37 |
26 |
29 |
|
23 |
12 |
39 |
150 |
37 |
32 |
||||||||
10 |
27 |
27 |
15 |
15 |
30 |
26 |
137 |
145 |
39 |
38 |
24 |
31 |
|
27 |
15 |
22 |
153 |
36 |
38 |
||||||||
50 |
24 |
27 |
13 |
14 |
24 |
24 |
115 |
136 |
38 |
31 |
28 |
27 |
|
30 |
14 |
24 |
157 |
23 |
26 |
||||||||
100 |
21 |
24 |
12 |
14 |
30 |
30 |
113 |
129 |
18 |
22 |
25 |
27 |
|
27 |
15 |
29 |
145 |
26 |
28 |
||||||||
500 |
24 |
26 |
10 |
12 |
30 |
31 |
131 |
137 |
35 |
33 |
22 |
27 |
|
28 |
14 |
31 |
142 |
31 |
31 |
||||||||
1000 P |
25 |
29 |
13 |
16 |
22 |
29 |
126 |
141 |
31 |
35 |
28 |
33 |
|
32 |
19 |
36 |
155 |
39 |
38 |
||||||||
5000 P |
33 |
29 |
18 |
21 |
29 |
27 |
153 |
155 |
33 |
40 |
35 |
34 |
|
24 |
24 |
25 |
157 |
47 |
33 |
||||||||
8000 P |
29 |
29 |
- |
- |
148 |
149 |
- |
- |
|||||
29 |
150 |
||||||||||||
Positive controls (µg/plate) |
AF-2 (0.05) |
9AA (80) |
4NQO (0.25) |
AF-2 (0.01) |
ENNG (5) |
AF-2 (0.05) |
|||||||
No. of colonies/plate |
263 |
255 |
957 |
843 |
186 |
187 |
471 |
435 |
1916 |
2048 |
987 |
1027 |
|
246 |
729 |
188 |
399 |
2180 |
1066 |
||||||||
+ |
Solvent control (DMSO) |
34 |
32 |
19 |
19 |
31 |
32 |
180 |
169 |
18 |
14 |
28 |
26 |
30 |
19 |
32 |
157 |
9 |
24 |
||||||||
1 |
40 |
44 |
12 |
12 |
35 |
36 |
163 |
158 |
12 |
14 |
26 |
29 |
|
48 |
12 |
36 |
152 |
15 |
31 |
||||||||
5 |
42 |
43 |
9 |
8 |
36 |
37 |
136 |
137 |
19 |
18 |
24 |
23 |
|
43 |
7 |
38 |
138 |
16 |
21 |
||||||||
10 |
33 |
35 |
23 |
17 |
27 |
27 |
137 |
142 |
11 |
14 |
20 |
24 |
|
36 |
11 |
27 |
147 |
16 |
27 |
||||||||
50 |
41 |
43 |
13 |
21 |
24 |
33 |
150 |
149 |
14 |
17 |
23 |
28 |
|
45 |
28 |
41 |
148 |
20 |
32 |
||||||||
100 |
34 |
34 |
19 |
21 |
33 |
35 |
140 |
151 |
19 |
19 |
35 |
34 |
|
34 |
23 |
36 |
162 |
19 |
32 |
||||||||
500 |
35 |
38 |
10 |
12 |
43 |
46 |
135 |
136 |
16 |
13 |
33 |
29 |
|
41 |
14 |
48 |
137 |
9 |
25 |
||||||||
1000 P |
44 |
45 |
16 |
17 |
39 |
41 |
156 |
160 |
15 |
17 |
41 |
40 |
|
45 |
17 |
42 |
163 |
18 |
39 |
||||||||
5000 P |
24 |
41 |
15 |
17 |
38 |
43 |
149 |
152 |
11 |
13 |
33 |
33 |
|
40 |
18 |
47 |
155 |
15 |
33 |
||||||||
8000 P |
45 |
46 |
- |
- |
150 |
151 |
- |
- |
|||||
47 |
151 |
||||||||||||
Positive controls (µg/plate) |
B(a)P (5) |
2AA(5) |
2AA(5) |
||||||||||
No. of colonies/plate |
853 |
801 |
289 |
320 |
270 |
276 |
1051 |
1021 |
764 |
780 |
256 |
245 |
|
749 |
350 |
281 |
990 |
796 |
234 |
2AA = 2-aminoanthracene
4NQO = 4-nitroquinoline-N-oxide
9AA = 9-aminoacridine
B(a)P = benzo(a)pyrene
ENNG = ethyl-N-nitro-N'- nitrosoguanidine
P = precipitate
Applicant's summary and conclusion
- Conclusions:
- Based on the results of the conducted study the test substance did not exhibit mutagenic properties in bacterial cells.
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