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EC number: 235-985-8 | CAS number: 13080-86-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://chesar.echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_toxicological-information.png)
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 10 - 29 Sep 1983
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
- Remarks:
- no information on historical data, acceptance and evaluation criteria; only 1 assay performed, tested up to 8000 µg/plate
Data source
Reference
- Reference Type:
- other: Summary of translation of japanese report
- Title:
- Unnamed
- Year:
- 1 983
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Version / remarks:
- adopted in 1997
- GLP compliance:
- no
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- 4,4'-[isopropylidenebis(4,1-phenyleneoxy)]dianiline
- EC Number:
- 235-985-8
- EC Name:
- 4,4'-[isopropylidenebis(4,1-phenyleneoxy)]dianiline
- Cas Number:
- 13080-86-9
- Molecular formula:
- C27H26N2O2
- IUPAC Name:
- 4,4'-[isopropylidenebis(4,1-phenyleneoxy)]dianiline
Constituent 1
Method
- Target gene:
- his operon, trp operon
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Species / strain / cell type:
- S. typhimurium TA 1538
- Species / strain / cell type:
- E. coli WP2 uvr A
- Metabolic activation:
- with and without
- Metabolic activation system:
- cofactor-supplemented post-mitochondrial fraction (S9 mix), prepared from the livers of male rats, induced with PCB (KC-500)
- Test concentrations with justification for top dose:
- Following concentrations were used in main experiment (preincubation):
all strains: 1, 5, 10, 50, 100, 500, 1000, 5000, 8000 μg/plate (with and without metabolic activation) - Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: DMSO
Controls
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- Remarks:
- DMSO
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- 4-nitroquinoline-N-oxide
- 9-aminoacridine
- benzo(a)pyrene
- other: AF-2; ethyl-N-nitro-N'- nitrosoguanidine (ENNG), 2-aminoanthracene (2AA)
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: preincubation
DURATION
- Preincubation period: 20 min
- Exposure duration: 48 h
NUMBER OF REPLICATIONS: duplicate
DETERMINATION OF CYTOTOXICITY
- Method: inspection of bacterial background lawn
Results and discussion
Test results
- Species / strain:
- other: S. typhimurium TA 98, TA 100, TA 1535, TA 1537, TA 1538, E. coli WP2 uvr A
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity, but tested up to precipitating concentrations
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- valid
- Additional information on results:
- TEST-SPECIFIC CONFOUNDING FACTORS
- Precipitation: Precipitation of the test substance was observed at a concentration of 1000 µg/plate.
Any other information on results incl. tables
Table 1: Test results (experiment 1, preincubation)
With or without S9 Mix |
Test substance concentration (μg/plate) |
Number of revertant colonies per plate |
|||||||||||
Frameshift type |
Base-pair substitution type |
||||||||||||
TA 98 |
TA 1537 |
TA 1538 |
TA 100 |
TA 1535 |
WP2 uvr A (pKM 101) |
||||||||
- |
Solvent control (DMSO) |
25 |
26 |
12 |
16 |
30 |
32 |
139 |
142 |
34 |
37 |
34 |
35 |
27 |
20 |
34 |
145 |
40 |
35 |
||||||||
1 |
24 |
27 |
19 |
21 |
30 |
30 |
107 |
127 |
33 |
34 |
25 |
25 |
|
29 |
22 |
30 |
147 |
34 |
24 |
||||||||
5 |
39 |
31 |
12 |
12 |
23 |
31 |
144 |
147 |
37 |
37 |
26 |
29 |
|
23 |
12 |
39 |
150 |
37 |
32 |
||||||||
10 |
27 |
27 |
15 |
15 |
30 |
26 |
137 |
145 |
39 |
38 |
24 |
31 |
|
27 |
15 |
22 |
153 |
36 |
38 |
||||||||
50 |
24 |
27 |
13 |
14 |
24 |
24 |
115 |
136 |
38 |
31 |
28 |
27 |
|
30 |
14 |
24 |
157 |
23 |
26 |
||||||||
100 |
21 |
24 |
12 |
14 |
30 |
30 |
113 |
129 |
18 |
22 |
25 |
27 |
|
27 |
15 |
29 |
145 |
26 |
28 |
||||||||
500 |
24 |
26 |
10 |
12 |
30 |
31 |
131 |
137 |
35 |
33 |
22 |
27 |
|
28 |
14 |
31 |
142 |
31 |
31 |
||||||||
1000 P |
25 |
29 |
13 |
16 |
22 |
29 |
126 |
141 |
31 |
35 |
28 |
33 |
|
32 |
19 |
36 |
155 |
39 |
38 |
||||||||
5000 P |
33 |
29 |
18 |
21 |
29 |
27 |
153 |
155 |
33 |
40 |
35 |
34 |
|
24 |
24 |
25 |
157 |
47 |
33 |
||||||||
8000 P |
29 |
29 |
- |
- |
148 |
149 |
- |
- |
|||||
29 |
150 |
||||||||||||
Positive controls (µg/plate) |
AF-2 (0.05) |
9AA (80) |
4NQO (0.25) |
AF-2 (0.01) |
ENNG (5) |
AF-2 (0.05) |
|||||||
No. of colonies/plate |
263 |
255 |
957 |
843 |
186 |
187 |
471 |
435 |
1916 |
2048 |
987 |
1027 |
|
246 |
729 |
188 |
399 |
2180 |
1066 |
||||||||
+ |
Solvent control (DMSO) |
34 |
32 |
19 |
19 |
31 |
32 |
180 |
169 |
18 |
14 |
28 |
26 |
30 |
19 |
32 |
157 |
9 |
24 |
||||||||
1 |
40 |
44 |
12 |
12 |
35 |
36 |
163 |
158 |
12 |
14 |
26 |
29 |
|
48 |
12 |
36 |
152 |
15 |
31 |
||||||||
5 |
42 |
43 |
9 |
8 |
36 |
37 |
136 |
137 |
19 |
18 |
24 |
23 |
|
43 |
7 |
38 |
138 |
16 |
21 |
||||||||
10 |
33 |
35 |
23 |
17 |
27 |
27 |
137 |
142 |
11 |
14 |
20 |
24 |
|
36 |
11 |
27 |
147 |
16 |
27 |
||||||||
50 |
41 |
43 |
13 |
21 |
24 |
33 |
150 |
149 |
14 |
17 |
23 |
28 |
|
45 |
28 |
41 |
148 |
20 |
32 |
||||||||
100 |
34 |
34 |
19 |
21 |
33 |
35 |
140 |
151 |
19 |
19 |
35 |
34 |
|
34 |
23 |
36 |
162 |
19 |
32 |
||||||||
500 |
35 |
38 |
10 |
12 |
43 |
46 |
135 |
136 |
16 |
13 |
33 |
29 |
|
41 |
14 |
48 |
137 |
9 |
25 |
||||||||
1000 P |
44 |
45 |
16 |
17 |
39 |
41 |
156 |
160 |
15 |
17 |
41 |
40 |
|
45 |
17 |
42 |
163 |
18 |
39 |
||||||||
5000 P |
24 |
41 |
15 |
17 |
38 |
43 |
149 |
152 |
11 |
13 |
33 |
33 |
|
40 |
18 |
47 |
155 |
15 |
33 |
||||||||
8000 P |
45 |
46 |
- |
- |
150 |
151 |
- |
- |
|||||
47 |
151 |
||||||||||||
Positive controls (µg/plate) |
B(a)P (5) |
2AA(5) |
2AA(5) |
||||||||||
No. of colonies/plate |
853 |
801 |
289 |
320 |
270 |
276 |
1051 |
1021 |
764 |
780 |
256 |
245 |
|
749 |
350 |
281 |
990 |
796 |
234 |
2AA = 2-aminoanthracene
4NQO = 4-nitroquinoline-N-oxide
9AA = 9-aminoacridine
B(a)P = benzo(a)pyrene
ENNG = ethyl-N-nitro-N'- nitrosoguanidine
P = precipitate
Applicant's summary and conclusion
- Conclusions:
- Based on the results of the conducted study the test substance did not exhibit mutagenic properties in bacterial cells.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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