Flue dust, zinc-refining

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Tests done according to standard protocol. Good quality and considered useful for setting the reference value for acute aquatic ecotoxicity. Culture medium, test water conditions well described and relevant the purpose of classification.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2006

Report date:

2006

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Flue dust, zinc-refining

EC Number:

273-760-6

EC Name:

Flue dust, zinc-refining

Cas Number:

69012-63-1

Molecular formula:

Not applicable - UVCB

IUPAC Name:

Zinc oxide enriched flue dust

Constituent 2

Reference substance name:

flue dust, zinc refining

IUPAC Name:

flue dust, zinc refining

Details on test material:

Waelz oxide was "ungelaucht"
zinc compounds >50%
lead compounds 5-10%
cadmium compounds < 0.25%

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

samples were taken at the start and end of test, and stabilised with HNO3

Test solutions

Vehicle:

no

Details on test solutions:

according to OECD 202. The test was performed on a "water soluble fraction" according to the OECD series on testing and assessment Number 23, : "Guidance document on aquatic toxicity of difficult substances and mixtures".
The water soluble fraction was prepared by stirring 100mg of waelz oxide in 1L Daphnid testing solution for 24hrs. After settling for 1hr, tyhe "water accommodated fraction" was taken from the middle of the solution, and filtered over a 0.2µm cellulose-acetate filter to get the "water soluble fraction". This was used in the test.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

D. magna originating from the Umweltbundesambt, abteilung V3.2. were used. The animals were fed 5 days a week with algae suspension consisting of Desmodesmus subspicatus. The culture was stabile.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Test conditions

Test temperature:

19.4-20°C

pH:

diffference between start and end of test was <0.5 pH units

Dissolved oxygen:

8.6-10.2 mg O2/l

Nominal and measured concentrations:

measured zinc concentration at end of test: 296µg/l (with 100mg/l loading).
measured Pb and Cd were 49µg/l and 58µg/l, respectively

Results and discussion

Effect concentrations

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

Tests done according to standard protocol. Good quality and considered useful for setting the reference value for acute aquatic ecotoxicity.

Executive summary:

A limit test according to OECD 202 with 100mg/l loading of Waelz zinc oxide demonstrated that the EC50 for Daphnia magna was > 100mg/l.