Flue dust, zinc-refining

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Tests done according to standard protocol. Good quality and considered useful for setting the reference value for acute aquatic ecotoxicity. Culture medium, test water conditions well described and relevant the purpose of classification.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2006

Report date:

2006

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Flue dust, zinc-refining

EC Number:

273-760-6

EC Name:

Flue dust, zinc-refining

Cas Number:

69012-63-1

Molecular formula:

Not applicable - UVCB

IUPAC Name:

Zinc oxide enriched flue dust

Details on test material:

"gelaucht" Waelzoxide

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

sample for measurement of metal concentration at begin and end of test.

Test solutions

Details on test solutions:

according to OECD 203. The test was performed on a "water soluble fraction" according to the OECD series on testing and assessment Number 23, : "Guidance document on aquatic toxicity of difficult substances and mixtures".
The water soluble fraction was prepared by stirring 100mg of waelz oxide in 1L fish testing solution for 24hrs. After settling for 1hr, the "water accommodated fraction" was taken from the middle of the solution, and filtered over a 0.2µm cellulose-acetate filter to get the "water soluble fraction". This was used in the test.

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

7 fish/concentration were used. They were fed 5 days/week with goldfish fodder (Tetramin from tetrawerke, Melle).tst fish were 3cm long.

Study design

Test type:

static

Water media type:

freshwater

Test conditions

Test temperature:

19.8-23.8°C

pH:

7.2-7.9

Dissolved oxygen:

7.9-10.4mg O2/L

Nominal and measured concentrations:

at nominal 100mg/l loading, a maximum concentration of 1280µg/l, 252µg/l, and 144µg/l was observed for zinc, lead and cadmium, resp.

Results and discussion

Effect concentrations

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

Tests done according to standard protocol. Good quality and considered useful for setting the reference value for acute aquatic ecotoxicity.

Executive summary:

Acute toxicity of Waelz oxide was determined on Brachydanio rerio. At 100mg/l loading, no toxicity was observed.