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Diss Factsheets

Ecotoxicological information

Toxicity to microorganisms

Currently viewing:

Administrative data

Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Study period:
May to August 1998
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1998
Report date:
1998

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
GLP compliance:
yes

Test material

Constituent 1
Reference substance name:
609-333-1
EC Number:
609-333-1
Cas Number:
37143-54-7
Molecular formula:
C4 H11 N O
IUPAC Name:
609-333-1
Details on test material:
- Batch number: 08099500
- Purity: 99,7 % (BASF report 97L00214)
- Homogeneity: homogeneous

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: A stock solution containing 2 g/L of the test substance was prepared. Corresponding amounts of the stock solution were added to the test vessels in order to achieve the desired test concentrations.

Test organisms

Test organisms (species):
other: Mixture of municipal and synthetic sewage taken from laboratory STP
Details on inoculum:
- Preparation of inoculum for exposure: The inoculum was washed, brought to a concentration of 5 g/L dry substance and aerated for 24 hours. 50 mL were added to a test volume of 250 mL to obtain a concentration of 1 g/L dry substance in the test.

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
30 min

Test conditions

Test temperature:
20 ± 2°C
pH:
8.5 - 10
Nominal and measured concentrations:
Nominal concentrations: 100, 500 and 1000 mg/L
Details on test conditions:
TEST SYSTEM
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 3

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Synthetic medium (8 mL/vessel 100-fold concentrated OECD medium)
Reference substance (positive control):
yes
Remarks:
3,5-dichlorophenol

Results and discussion

Effect concentrationsopen allclose all
Duration:
30 min
Dose descriptor:
other: EC20
Effect conc.:
ca. 700 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Duration:
30 min
Dose descriptor:
EC50
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Duration:
30 min
Dose descriptor:
other: EC80
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Results with reference substance (positive control):
- Results with reference substance valid? yes
- EC50: ca. 17 mg/L

Applicant's summary and conclusion

Validity criteria fulfilled:
yes