1-Propanaminium, 3-amino-N-(carboxymethyl)-N,N-dimethyl-, N-C8-10 (even numbered) acyl derivs., hydroxides, inner salts

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

12.05.1992-04.06.1992

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Remarks:

without analytical monitoring

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Version / remarks:

part I, 24h EC50 Acute Immobilization Test (1984)

GLP compliance:

yes

Analytical monitoring:

no

Vehicle:

no

Test organisms (species):

Daphnia magna

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

ca. 1.9 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

mobility

Remarks on result:

other: 95% CL: 1.37 - 2.69 mg a.i./L

Reported statistics and error estimates:

The EC50 value was calculated using the Litchfield Wilcoxon method.

RESULTS: EXPOSED
- Nominal/measured concentrations: nominal
- Effect data (Immobilization):
concentration         number of       number of animals
(mg/l)                animals         immobilized after 48h
 0.5                   20                 0
 1                     40                 2
 2                     40                 3
 4                     20                 6
 8                     20                 9
16                     20                20

Based on the number of immobilized animals after 48h, the EC50 value was  
calculated using the method of Litchfield and Wilcoxon.

48 h EC50:   6.4  mg product/L 
          with 95% confidence limit of 4.57 - 8.96 mg product/L

Assuming a content of 30% active substance:
48 h EC50:   ca. 1.9  mg active substance/L 
          with confidence limit of 1.37 - 2.69 mg a.i./L

Validity criteria fulfilled:

yes

Conclusions:

The acute toxicity of Coco AAPB to Daphnia magna was investigated in a study conducted according to OECD guideline 202. The 48 h EC50 was determined to be ca. 1.9 mg a.i./L nominal.

Executive summary:

The acute toxicity of Coco AAPB to Daphnia magna was investigated in a study conducted according to OECD TG 202 / I under static conditions. Daphnia magna was exposed to 6 nominal concentrations ranging from 0.5 to 16 mg product/L. A 48 h LC50 of ca. 1.9 mg a.i/L nominal was determined based on an active matter content of 30%.

This study is regarded as reliable with restrictions.

Results Synopsis
Test Organism/Age (e.g. 1^stinstar): Daphnia magna/<24 h
Test Type: Static
48 h EC50:  ca. 1.9 mg a.i./L; 95% C.I.:  1.37-2.69 mg a.i./L (active matter assumed: 30%; values refer to nominal concentrations)
Endpoint(s) Effected: Immobilization

Description of key information

48 h EC50 = 1.9 mg a. i./L (nominal), OECD TG 202 / part I (1984) static, Daphnia magna, GLP, RL2; read-across: C8-18 and C18 unsatd. AAPB

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

1.9 mg/L

Additional information

No experimental data are available for the target substance C8-10 Alkylamidopropyl betaine. However, short-term toxicity studies with aquatic invertebrates are available for the source substances Formamidopropylbetaine, C8-18 AAPB and C8-18 and C18 unsatd. AAPB. A justification for read-across is given in the Endpoint summary “Aquatic Toxicity”.

The acute toxicity of C8-18 and C18 unsatd. AAPB to Daphnia magna was investigated in a study conducted according to OECD TG 202 / part I (1984) under static conditions. Daphnia magna was exposed to 6 nominal concentrations ranging from 0.5 to 16 mg product/L. A 48 h LC50 of ca. 1.9 mg a.i/L (nominal) was determined based on an active matter content of 30%.

The 48 hr-acute toxicity of C8-18 AAPB (30% active matter) to Daphnia magna was investigated in a study conducted according to OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test) and EU Method C.2 (Acute Toxicity for Daphnia) under static conditions. Daphnids were exposed to control and test chemical at nominal concentrations of 6.25; 12.5; 25; 50; 100 mg product/L for 48 hr. The test material concentrations were analysed via UV absorption at 485 nm. The C8-18 AAPB concentrations were >80% (exception: 72.2% at the start in the 6.25 mg product/L group). The 48 h EC50 was determined to be 6.5 mg a.i./L based on nominal concentrations.

The acute toxicity of Formamidopropylbetaine to Daphnia magna was investigated in a study conducted according to OECD Guideline 202. The substance did not induce acute immobilisation of Daphnia magna at 100 mg/L after 48 hours of exposure. The 48h-EC50 was > 100 mg/L based on analytically confirmed nominal exposure concentrations.

Conclusion

For freshwater invertebrates (Daphnia magna) the lowest 48 h EC50 was determined to be 1.9 mg a. i./L (nominal).