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Diss Factsheets
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EC number: 944-170-2 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to microorganisms, other
- Remarks:
- toxicity control from biodegradation in water screening test is used to derive effect concentration
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2004-12-02 to 2004-12-31
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Reason / purpose for cross-reference:
- reference to same study
- Qualifier:
- according to guideline
- Guideline:
- other: OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test) (adopted July 17, 1992)
- Deviations:
- no
- Principles of method if other than guideline:
- From biodegradation in water: screening test, toxicity control is used to derive effect concentration
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- no
- Vehicle:
- no
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Municipal sewage treatment plant, D-31137 Hildesheim
- Pretreatment: The activated sludge was maintained in an aerobic condition by aeration for four hours and then homogenized with a mixer. The sludge was filtered and the filtrate (30 mL) was subsequently used to initiate inoculation.
- Concentration of sludge: 1E07-1E08 CFU/L in inoculum; 1E05-1E06 CFU/L in test vessels - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 28 d
- Test temperature:
- 20 - 24°C
- pH:
- 7.56 - 7.59 (test item), 7.51 - 7.59 (control), 7.86 (functional control), 7.83 (toxicity control)
- Nominal and measured concentrations:
- 45 mg/L (11.8 mg C/L), nominal
- Details on test conditions:
- TEST CONDITIONS
- Composition of medium: Mineral nutrient solution acc. to OECD 301 B/CO2 Evolution Test
- Additional substrate: no
- Test temperature: 20 - 24°C, continuously stirred
- pH: 7.56 - 7.59 (test item), 7.51 - 7.59 (control), 7.86 (functional control), 7.83 (toxicity control)
- pH adjusted: no
- Continuous darkness: yes
TEST SYSTEM
- Culturing apparatus: 5000 mL, brown glass; fill volume 3000 mL
- Number of culture flasks/concentration: dupilcate (test item)
- Method used to create aerobic conditions: The vessels were connected to the system for the production of CO2 free air and aerated for 24 h.
- Details of trap for CO2 and volatile organics if used: The CO2 adsorption vessels were connected to the air outlets of the incubation vessels via a series of 3 gas wash bottles.
SAMPLING
- Sampling frequency: three times a week during the first ten days and thereafter twice weekly + on day 29
CONTROL AND BLANK SYSTEM
- Inoculum blank: Nutrient solution and inoculum, duplicates
- functional control: Sodium acetate, 35 mg/L (10.2 mg C/L), single replicate
- Toxicity control: Sodium acetate, 35 mg/L (10.2 mg C/L) + test item 45 mg/L (11.8 mg C/L), single replicate - Reference substance (positive control):
- no
- Key result
- Duration:
- 14 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 45 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Remarks on result:
- other: comparison of procedural and toxicity control
- Details on results:
- The biodegradation of the item mixture in the toxicity control was found to be 63 % after 14 days of incubation and came to a maximum of 85 % after 28 days. Thus, according to the test guidelines, the test item had no inhibitory effect on activated sludge microorganisms at the tested concentration of 45 mg/L, as the biodegradation in the toxicity control was higher than 35% within 14 days of incubation.
- Validity criteria fulfilled:
- yes
- Conclusions:
- The toxicity of C8-10 Alkylamidopropyl betaine to microorganisms was investigated during a ready biodegradation study. The test item had no inhibitory effect on activated sludge microorganisms at the tested concentration of 45 mg/L.
- Executive summary:
The toxicity of C8-10 Alkylamidopropyl betaine (35% a.i.) to microorganisms was investigated during a ready biodegradation study (CO2 Evolution Test) according to OECD guideline 301 B (1992) over a period of 28 days and using an inoculum obtained from activated sludge from a predominantly domestic sewage treatment plant.
Inoculum blank, procedural/functional control with the reference substance Sodium acetate and a toxicity control with 45 mg/L test item and 35 mg/L reference item Sodium acetate were performed.
The reference item degraded normally (68% within 14 days). By the end of the test, the reference item was degraded by 82%.
The biodegradation of the item mixture in the toxicity control was found to be 63% after 14 days of incubation. Thus, according to the test guidelines, the test item had no inhibitory effect on activated sludge microorganisms at the tested concentration of 45 mg/L, as the biodegradation in the toxicity control was higher than 35% within 14 days of incubation.
Reference
Description of key information
14 d NOEC > 45 mg a.i./L, OECD TG 301 B, GLP, RL1
Key value for chemical safety assessment
- EC10 or NOEC for microorganisms:
- 45 mg/L
Additional information
The toxicity of C8-10 Alkylamidopropyl betaine (35% a.i.) to microorganisms was investigated during a ready biodegradation study (CO2 Evolution Test) according to OECD guideline 301 B (1992) over a period of 28 days and using an inoculum obtained from activated sludge from a predominantly domestic sewage treatment plant.
Inoculum blank, procedural/functional control with the reference substance Sodium acetate and a toxicity control with 45 mg/L test item and 35 mg/L reference item Sodium acetate were performed.
The reference item degraded normally (68% within 14 days). By the end of the test, the reference item was degraded by 82%.
The biodegradation of the item mixture in the toxicity control was found to be 63% after 14 days of incubation. Thus, according to the test guidelines, the test item had no inhibitory effect on activated sludge microorganisms at the tested concentration of 45 mg/L, as the biodegradation in the toxicity control was higher than 35% within 14 days of incubation.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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