Trisodium 2-[[4-[[4-[(4-amino-6-chloro-1,3,5-triazin-2-yl)amino]-5-sulphonatonaphthyl]azo]-2,5-dimethylphenyl]azo]benzene-1,4-disulphonate

Water solubility

Link to relevant study record(s)

Reference

Endpoint:

water solubility

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2014

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 105 (Water Solubility)

Deviations:

no

Qualifier:

according to guideline

Guideline:

EU Method A.6 (Water Solubility)

Deviations:

no

GLP compliance:

no

Type of method:

flask method

Specific details on test material used for the study:

Test Item: FAT 40075/D
Lot: BOP 01-12

Water solubility:

334 g/L

Temp.:

20 °C

Remarks on result:

other: pH is not known

Preliminary Visual Estimation of the Water Solubility

Appearance of the test mixtures in the preliminary test

Amount of the test item in mg	Total volume in ml	Appearance of mixture
100	0.1	Undissolved
	0.5	Dissolved

 

The preliminary visual experiments showed that the water solubility of the test item is >200 g/l and <1000 g/l . Therefore the water solubility had to bo determined by the “flask method”. Due to the high water solubility it was tested which amount of the test substance could be stirred in a defined volume of water.

 

Appearance of the test mixtures in the stirring test

volume of waterin ml	mass of the added test item in g	total mass of the test item in g	Appearance of mixture
2	1.0	1.0	solution is stirrable
2	+0.5	1.5	solution is stirrable
2	+0.5	2.0	could not be stirred
2.5	---	2.0	solution is stirrable

 

Due to this stirring experiment the main test could be performed with 2.0 g in 5 ml water (400 g/l) This solution was stirred at 20 °C for 22 hours. After this time a dark coloured pasty solution was observed. No precipitate was observed. The stirring test showed that 400 g/l test item can be used for flask method.

 

Flask-Method

Water solubility of the test item – experiments A to D

Experiment	A	B	C	D (blank)
Amount of test item (g)	2.0	2.0	2.0	---
Stirring time at 30 °C (h)	24	48	72	72
Aliquot of the flasks μl/10/0.40/10	10.0	10.0	10.0	10.0
Measured concentration (μg/ml)	13.2	13.6	13.3	---
Water solubility (g/l)	328	341	332	---

 

Average experiments A to C: 334 g/l

Standard deviation: 10.2 g/l

Relative standard deviation: 3.1 %

Conclusions:

The water solubility of the test item at a temperature of 20 °C was determined to be 334 g/l.

Executive summary:

The water solubility of FAT 40075/D was evaluated in a study conducted according to OECD Guideline 105. Flask method was used for this purpose. based on the findings of the study, the water solubility of the test item at a temperature of 20 °C was determined to be 334 g/l.

Description of key information

The water solubility of FAT 40075/D was evaluated in a study conducted according to OECD Guideline 105. Flask method was used for this purpose. Based on the findings of the study, the water solubility of the test item at a temperature of 20 °C was determined to be 334 g/l.

Key value for chemical safety assessment

Water solubility:

334 g/L

at the temperature of:

20 °C