Sodium 1-amino-9,10-dihydro-4-[5-[(2-hydroxyethyl)sulphamoyl]-3,4-xylidino]-9,10-dioxoanthracene-2-sulphonate

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1981

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Version / remarks:

Comparable to internationally accepted guidelines.

Deviations:

no

Principles of method if other than guideline:

Method A-11

GLP compliance:

no

Specific details on test material used for the study:

FAT 20033/G
Purity: approx. 82.2 %

Analytical monitoring:

no

Details on sampling:

None

Vehicle:

no

Details on test solutions:

none

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

10 fishes were used per concentration.

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Remarks on exposure duration:

none

Post exposure observation period:

none

Test temperature:

25 °C

pH:

8.2 - 8.3

Dissolved oxygen:

6.7 - 7.1

Nominal and measured concentrations:

30, 100 and 300 mg/L

Reference substance (positive control):

no

Duration:

96 h

Dose descriptor:

other: TL0

Effect conc.:

30 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

other: TL50

Effect conc.:

113 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

other: TL100

Effect conc.:

300 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

After 96h exposure, 10/10, 7/10 and 0/10 fish survived at 30, 100 and 300 mg/L, respectively.

Validity criteria fulfilled:

not specified

Conclusions:

The 96h TL50 of FAT 20033/G in fish acute toxicity test was determined to be 113 mg/L.

Executive summary:

The acute fish toxicity test was performed using a methodology equivalent or similar to OECD Guideline 203. Groups of fish were exposed to FAT 20033/G for 96 hours at following concentrations: 30, 100 and 300 mg/L.

After 96h exposure, 10/10, 7/10 and 0/10 fishes survived at 30, 100 and 300 mg/L, respectively. Based on the findings of the study, the 96h TL50 of FAT 20033/G in this fish acute toxicity test was determined to be 113 mg/L.

Description of key information

The 96h LC50 of Acid Blue 277 was determined to be 113 mg/L.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Dose descriptor:

LC50

Effect concentration:

113 mg/L

Additional information

A study was conducted to determine the acute toxicity of the test substance (of ca.82.2 %) to zebrafish (Danio rerio). Groups of 10 fishes were exposed at 30, 100 or 300 mg/L for a duration of 96 h. The number of surviving fish was recorded at 4, 24, 48, 72 and 96 h. All fish died within 4 h at the high dose whereas 3 fish died at the intermediate dose and none at the low dose. Under the test conditions, the 96 h LC0 was 30 mg/L (i.e. ca. 25 mg a.i./L), the 96 h LC50 was 113 mg/L (i.e. ca. 93 mg a.i./L) and the 96 h LC100 was 300 mg/L (i.e. ca. 247 mg a.i./L).