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Toxicological information

Repeated dose toxicity: other routes

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Administrative data

Endpoint:
short-term repeated dose toxicity: other route
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Adopted according to OECD SIDS (public available peer reviewed source) and respective study summary. The original source is not available and has not been reviewed.

Data source

Referenceopen allclose all

Reference Type:
publication
Title:
n-octane and n-nonane induced alterations in xenobiotic metabolising enzyme activities and lipid peroxidation of rat liver.
Author:
Khan, S. et al.
Year:
1980
Bibliographic source:
Toxicology 16: 239-245
Reference Type:
secondary source
Title:
OECD SIDS - Category: C7-C9 Aliphatic hydrocarbon solvents
Author:
IHSC, American Chemistry Council
Year:
2009
Bibliographic source:
SIDS Initial Assessment Report For SIAM 30 Paris, 20-23 April 2010, drafted by members of the IHSC and reviewed by the U.S. Environmental Protection Agency (EPA), October 28, 2009
Report date:
2009

Materials and methods

Principles of method if other than guideline:
Purpose of the study was to evaluate enzyme activity and potential for hepatotoxicity. Animals were treated with octane for 2 or 7 days by intraperitoneal injections.
GLP compliance:
not specified
Limit test:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
Octane
EC Number:
203-892-1
EC Name:
Octane
Cas Number:
111-65-9
Molecular formula:
C8H18
IUPAC Name:
octane
Details on test material:
- Name of test material (as cited in study report): n-octane
- Analytical purity: 99%

Test animals

Species:
rat
Strain:
not specified
Sex:
female

Administration / exposure

Route of administration:
intraperitoneal
Vehicle:
not specified
Analytical verification of doses or concentrations:
yes
Duration of treatment / exposure:
2 or 7 days
Frequency of treatment:
daily
Doses / concentrations
Remarks:
Doses / Concentrations:
1 mL/kg (approx. 703 mg/kg octane)
No. of animals per sex per dose:
6
Control animals:
yes

Results and discussion

Target system / organ toxicity

Critical effects observed:
not specified

Any other information on results incl. tables

Since only a single dose was administered it is not possible to derive a NOAEL.

 

Actual dose received by dose level: 1.0 mL/kg Toxic response/effects by dose level: After 2 and 7 day treatment with 1.0 mL/kg of n-octane by intraperitoneal injection, 50-80% reduction in specific activities of benzo(a)pyrene hydroxylase, benzphetamine - N-demethylase, p-nitroanisole -o-demethylase and glutathione -S-transferase were observed. Cytochrome P450 (31%) and total (31%) and free (49%) sulfhydryl content of the liver also decreased significantly. A 2 to 3-fold increase in liver lipid peroxidation was observed with exposure for 2 or 7 days to n-octane. n-Octane significantly affected the biotransformation mechanisms of liver microsomes.The toxicological implications of these observations require further study.

 

Similar effects were observed for n-nonane.

Applicant's summary and conclusion

Conclusions:
n-Octane administered at only 1.0 mL/kg/day intraperitoneally for 2 or 7 days resulted in decreases in levels of hepatic xenobiotic metabolizing enzymes and increases in liver lipid peroxidation.
Executive summary:

n-Octane administered at only 1.0 mL/kg/day intraperitoneally for 2 or 7 days resulted in decreases in levels of hepatic xenobiotic metabolizing enzymes and increases in liver lipid peroxidation.