Octane

Repeated dose toxicity: other routes

Administrative data

Endpoint:

short-term repeated dose toxicity: other route

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Adopted according to OECD SIDS (public available peer reviewed source) and respective study summary. The original source is not available and has not been reviewed.

Data source

Reference

Reference 1

Reference Type:

publication

Title:

n-octane and n-nonane induced alterations in xenobiotic metabolising enzyme activities and lipid peroxidation of rat liver.

Author:

Khan, S. et al.

Year:

1980

Bibliographic source:

Toxicology 16: 239-245

Reference 2

Reference Type:

secondary source

Title:

OECD SIDS - Category: C7-C9 Aliphatic hydrocarbon solvents

Author:

IHSC, American Chemistry Council

Year:

2009

Bibliographic source:

SIDS Initial Assessment Report For SIAM 30 Paris, 20-23 April 2010, drafted by members of the IHSC and reviewed by the U.S. Environmental Protection Agency (EPA), October 28, 2009

Report date:

2009

Materials and methods

Principles of method if other than guideline:

Purpose of the study was to evaluate enzyme activity and potential for hepatotoxicity. Animals were treated with octane for 2 or 7 days by intraperitoneal injections.

GLP compliance:

not specified

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Octane

EC Number:

203-892-1

EC Name:

Octane

Cas Number:

111-65-9

Molecular formula:

C8H18

IUPAC Name:

octane

Details on test material:

- Name of test material (as cited in study report): n-octane
- Analytical purity: 99%

Test animals

Species:

rat

Strain:

not specified

Sex:

female

Administration / exposure

Route of administration:

intraperitoneal

Vehicle:

not specified

Analytical verification of doses or concentrations:

yes

Duration of treatment / exposure:

2 or 7 days

Frequency of treatment:

daily

Doses / concentrations

Remarks:

Doses / Concentrations:
1 mL/kg (approx. 703 mg/kg octane)

No. of animals per sex per dose:

6

Control animals:

yes

Results and discussion

Target system / organ toxicity

Critical effects observed:

not specified

Any other information on results incl. tables

Since only a single dose was administered it is not possible to derive a NOAEL.

 

Actual dose received by dose level: 1.0 mL/kg Toxic response/effects by dose level: After 2 and 7 day treatment with 1.0 mL/kg of n-octane by intraperitoneal injection, 50-80% reduction in specific activities of benzo(a)pyrene hydroxylase, benzphetamine - N-demethylase, p-nitroanisole -o-demethylase and glutathione -S-transferase were observed. Cytochrome P450 (31%) and total (31%) and free (49%) sulfhydryl content of the liver also decreased significantly. A 2 to 3-fold increase in liver lipid peroxidation was observed with exposure for 2 or 7 days to n-octane. n-Octane significantly affected the biotransformation mechanisms of liver microsomes.The toxicological implications of these observations require further study.

 

Similar effects were observed for n-nonane.

Applicant's summary and conclusion

Conclusions:

n-Octane administered at only 1.0 mL/kg/day intraperitoneally for 2 or 7 days resulted in decreases in levels of hepatic xenobiotic metabolizing enzymes and increases in liver lipid peroxidation.

Executive summary:

n-Octane administered at only 1.0 mL/kg/day intraperitoneally for 2 or 7 days resulted in decreases in levels of hepatic xenobiotic metabolizing enzymes and increases in liver lipid peroxidation.