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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
other information
Study period:
1993-08-11-1993-11-26
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1993
Report date:
1993

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to guideline
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Qualifier:
according to guideline
Guideline:
other: OECD-Guideline for testing chemicals, 203 fish, Acute toxicity Test
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Reference substance name:
Benzoic acid,5-fluoro-2-nitro-, methyl ester
IUPAC Name:
Benzoic acid,5-fluoro-2-nitro-, methyl ester
Constituent 2
Chemical structure
Reference substance name:
5-Fluoro-2-nitrobenzoic acid methyl ester
EC Number:
609-664-1
Cas Number:
393-85-1
Molecular formula:
C8H6FNO4
IUPAC Name:
5-Fluoro-2-nitrobenzoic acid methyl ester
Test material form:
other: amber liquid
Details on test material:
Purity: 96+/-0.2 %

Sampling and analysis

Details on sampling:
In order to determine the substance concentration, water samples were taken from the middle of the test chamber by pipetting approx. 20 ml into a screw neck glass bottle at the study start, and after 48h and 96 h.

Test solutions

Details on test solutions:
All tested concentrations were present as clear solutions. All batches were homogeneous. No particular matter was observed.

Test organisms

Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
Feeding: twice ad libitum, The animals were kept at least 14 days before the study start of the study in the water for dilution

Study design

Test type:
static
Limit test:
no
Total exposure duration:
96 h

Test conditions

Hardness:
The total hardness of the dilution was determined weekly and was in the range of 2.1-2.3 mmol Ca2+ and Mg2+ /l during the study.
Test temperature:
22+/-1 °C
pH:
Concentration groups: 8.0-8.3
Control groups: 7.9-8.1
Details on test conditions:
The study was conducted in a static system. The test chambers were calibrated to 10 liters, were made of glass (length 30 cm, width 22 cm, height 24 cm ) and stood in a water bath made of Hostalit Z R with a plexiglas viewing panel. The chambers were illuminated from above from 6.00 a.m. to 6.00 p.m.After the test concentrations had been prepared and water parameter recorded 7 fish were assigned to each test and control chamber.

Results and discussion

Effect concentrationsopen allclose all
Duration:
24 h
Dose descriptor:
LC50
Effect conc.:
22 - 50 mg/L
Nominal / measured:
nominal
Duration:
48 h
Dose descriptor:
LC50
Effect conc.:
22 - 50 mg/L
Nominal / measured:
nominal
Duration:
72 h
Dose descriptor:
LC50
Effect conc.:
10 - 22 mg/L
Nominal / measured:
nominal
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
10 - 22 mg/L
Nominal / measured:
nominal
Details on results:
The fish showed partly changes in appearance and behaviour, swimming behaviour and respiration rate. Lethality occurred with a delay in the 10 mg/l and 22 mg/l group.

Applicant's summary and conclusion

Conclusions:
The LC50 after 24 hours was determined to be 22-50 mg/l.