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Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
other information
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2004
Report date:
2004

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: EC Directive 92/69/EC Method C.3, Official Journal of the European Communities No. L383A/179
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
5-Fluoro-2-nitrobenzoic acid methyl ester
EC Number:
609-664-1
Cas Number:
393-85-1
Molecular formula:
C8H6FNO4
IUPAC Name:
5-Fluoro-2-nitrobenzoic acid methyl ester
Test material form:
other: yellowish liquid
Details on test material:
Purity: 96.6 %

Test organisms

Test organisms (species):
Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)

Study design

Test type:
static
Limit test:
no
Total exposure duration:
72 h

Test conditions

Test temperature:
23+/- 2 °C
Nominal and measured concentrations:
Nominal concentration: 3.13- 6.25 -12.5-25-50-100 mg/L
Details on test conditions:
Light regime: 24 h/day, Temperature: 23+/- 2 °C, replicates: 3 per test condition and 6 control replicates, test volume: 100 ml

Results and discussion

Reported statistics and error estimates:
The BONFERRONI t-test was carried out for the determination of statistically significant differences compared to control replicates. When running a BONFERRONI t-test a Normaility test and Homogeneity test were done first. P-value for both were 0.05. The alpha value for the BONFERRONI t-test was 0.05.

Applicant's summary and conclusion

Conclusions:
In this study 5-Fluoro-2-nitrobenzoic acid methyl ester was found to inhibit the growth of the freshwater green alga Desmodesmus Subspicatus at nominal concentrations > 12 .5 mg/L (biomass and growth rate). The EC50 values with 95 % confidence intervals for inhibition of biomass growth (Eb50) and specific growth rate (Er50) after 72 h were 12.6 (12.2 -13.1) and 14.5 (14.1 -14.9) mg/l.